Keywords
hemoglobin standard
How to Cite
Abstract
Hepatitis B and HIV negative human blood samples were selected in order to evaluate the conditions of stable lysates in the monitoring of hemoglobin quantification. For this purpose, we employed a freezing-defrosting system which generated an inexpensive hemoglobin standard, stable for 6 months. This methodology could be sucessfully used in the quality control of all laboratories involved with hemoglobinic quantification.
References
1. BAUER, J.D. – Clinical laboratory methods. 9th. ed. St. Louis, C.V. Mosby, 1982. p. 46-7.
2. DACIE, J. V. & LEWIS, S.M. - Practical haematology, 5th. ed. London, Churchill Livingstone, 1975. p. 31-7, 60.
3. LEHNINGER, A.L. - Princípios de bioquímica. 5ª. ed. São Paulo, Sarvier, 1989. p. 136.
4. LEWIS, S.M. - El plan internacional de Ia OMS de evaluacion externa de la calidad en hematologia. Bol. Of. Sanit, Panam. 106 (5): 407-19, 1989.
5. LEWIS, S.M. - The principles and methods of quality assurance in haematology, Geneva, WHO, 1984. (WHO/LAB/84.3).
6. LEWIS, S.M. - Quality assurance in haematology: a training course manual practical work-book. Geneva, WHO, 1984. (WHO/LAB/84.6).
7. LEWIS, S.M. & BURGUESS, B.J. – Quality control in haematology: report of interlaboratory trials in Britain. Brit. Med. J. 4: 253-6, 1969.
8. SABRAFEN, J.S. - Hematologia clínica. 3º. ed. Barcelona, 1982. p. 5, 27-9, 189-90.
9. VAN ASSENDELFT, O.W.; HOLTZ, A.H. & LEWIS, S.M. - Recommended method for the determination of the haemoglobin concentration of blood. Geneva, WHO, 1984. (WHO/LAB/84.1O).
10. VAN KAMPEN, E.J. & ZIJLISTRA, W.G. - Standardization of hemoglobinometry II. The hemiglobincyanide method. Clin. chin. Acta. 6: 538-44, 1961.
11. ZIJLSTRA, W.G. & VANKAMPEN, E.J.- Standardization of hemoglonometry I. The extinction coefficient of hemiglobincyanide. Clin. chim. Acta. 5: 719-26, 1960.
This work is licensed under a Creative Commons Attribution 4.0 International License.
Copyright (c) 1991 Revista do Instituto Adolfo Lutz