Keywords
Candida
environmental contamination
Hospital
essencial oil from Cymbopogon citrates
How to Cite
Abstract
This study aimed at evaluating the antifungal activity of glucoprotamine solution (GLU) and the
essential oil from Cymbopogon citratus (EO). Candida tropicalis, Claviceps lusitaniae, Meyerozyma
(Pichia) guilliermondii and Candida parapsilosis were isolated from the samples, which were collected
from the surfaces of a public hospital located in Pelotas, RS. The antifungal activity of GLU was
detected at the concentrations from 0.25% to 0.0005%, while the EO, by Clevenger, was used at
concentrations from 17.500 μg mL to 0.034 μg/mL. Chemical analysis was determined by GC/MS.
The anti-Candida activity was performed by using Broth Microdilution technique to determine
the Minimal Fungicidal Concentration (MFC) and the Minimal Inhibitory Concentration (MIC).
The inhibition by GLU occurred at all of the tested concentrations, whereas the EO showed MIC
ranging from 2.846 to 5.450 μ/mL and the CFM from 2.853 to 5.330 μg/mL. Alfa–citral (37.10%),
beta–citral (32.77%) and beta-mirceno (18.75%) were the majority compounds in the EO. The data
obtained in the present study highlight the effectiveness of the solution based on GLU and EO for
eliminating the environmental isolates of Candida spp.
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