Avaliação das reações de Hemaglutinação Indireta (HI) e Aglutinação Modificada (MAT) na detecção de anticorpos IgG anti-T. gondii em exsudatos cárneos bovinos

Maria Aparecida Moraes  Marciano; Heitor Franco de  Andrade Junior; Luciana Regina  Meirelles

doi:10.53393/rial.2019.v78.35890

Vol. 78 (2019), BRIEF COMMUNICATION

Vol. 78 (2019)

Evaluation of indirect Hemagglutination (HI) and Modified Agglutination (MAT) reactions in the detection of anti-T. gondii IgG antibodies in bovine meat exudates

BRIEF COMMUNICATION

https://doi.org/10.53393/rial.2019.v78.35890

Published 2019-03-29

Maria Aparecida Moraes Marciano⁺⁻
Heitor Franco de Andrade Junior⁺⁻
Luciana Regina Meirelles⁺⁻

Maria Aparecida Moraes Marciano

Núcleo de Morfologia e Microscopia, Centro de Alimentos,Instituto Adolfo Lutz, São Paulo, SP, Brasil

Heitor Franco de Andrade Junior

Laboratório de Protozoologia do Instituto de Medicina Tropical de São Paulo, Universidade de São Paulo

Luciana Regina Meirelles

Laboratório de Protozoologia do Instituto de Medicina Tropical de São Paulo, Universidade de São Paulo

PDF (Português (Brasil))

Keywords

toxoplasmosis
meat exudate
food security
agglutination tests

How to Cite

1.

Marciano MAM, Andrade Junior HF de, Meirelles LR. Evaluation of indirect Hemagglutination (HI) and Modified Agglutination (MAT) reactions in the detection of anti-T. gondii IgG antibodies in bovine meat exudates. Rev Inst Adolfo Lutz [Internet]. 2019 Mar. 29 [cited 2024 Jul. 22];78(1):1-6. Available from: https://periodicos.saude.sp.gov.br/RIAL/article/view/35890

Abstract

Toxoplasmosis is a parasitic zoonosis with a wide worldwide distribution caused by
Toxoplasma gondii, which is considered one of the most successful protozoa on the planet,
since it can infect a third of the world population. Among the forms of transmission,
consumption of undercooked meat has been considered as a risk factor for the acquisition of
this zoonosis. An alternative approach to toxoplasmosis control by beef ingestion could be the
serological diagnosis in cattle, since seropositives animals harbor tissue cysts. However, the
use of serum for this evaluation is not always feasible due to the difficulty of blood collection
during slaughter and its absence in commercial beef cuts. Meat exudate is an alternative for
the detection of anti-T. gondii antibodies in commercial beef cuts, which was the propose of
this study to evaluate the performance of Indirect Hemagglutination (HI) and Agglutination
Modified (MAT) tests compared to ELISA using meat exudates. This study showed that the
agglutination tests accuracy was not viable due to low sensitivity and specificity indexes when
compared to ELISA. These data demonstrate the importance of choosing accurate tests such as
ELISA for application in quality control and safety of commercial beef cuts.

https://doi.org/10.53393/rial.2019.v78.35890

PDF (Português (Brasil))

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