Keywords
Multiplicitles of infections
Standardization measles antigen
Standardization measles antigen
How to Cite
1.
Isabel de Oliveira M, Rehder de Andrade Vaz de Lima L, Patriota de Andrade G, Fernando de Macedo Brígido L, Zucatelli Mendonça R. The role of M.O.I. in standardization of measles antigen preparation. Rev Inst Adolfo Lutz [Internet]. 1995 Jun. 30 [cited 2024 Jul. 22];55(1):25-9. Available from: https://periodicos.saude.sp.gov.br/RIAL/article/view/36559
Abstract
Measles is a prevalent and potencially sever disease in developing countries. The two methods widely used for diagnosis are ELISA and lHA. We studied different multiplicities of infections (m.o.i) to determine the ideal moi for the preparation of antigens for these assays. We conclude that the use of low (m.o.i.ü.O 1) viral titers are most useful for both the ELISA and lHA methods. The different preparation of antigens for these assays do not seems to influence their usage.
References
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12. VOLLER, A. & BIDWELL, D.E. - Enzymeimmunoassay for antibodies in measles, cytomegalovirus infection and after rubelIa vaccination.Br. 1.Exp. Panthol., 57:243-7,1976.
13. UDEM, S.A. -Measles virus: Conditions for the propagation and purification of infectious vírus in high yield. J. Virological Methods. 8: 123-136, 1984.
2 CHEN, R.T.; MARKOWITZ, L.E.; ALBRECHT, P.; JOHN, A.S. MOFENSON, L.M.; PREBLUD, S.R. & ORENSTEIN, - W.A.
Measles antigen: Reevaluation of protective titers. J. Infect. Dis. 162:1036-1042,1990.
3. CREMER, N.E.; COSSEN, C.K.; SHELL, G.; DIGGS, J.; GALLO, D. & SCHMIDT, N.J. Enzyme Immunoassay versus plaque neutralization and other methods for determination of immune status to measles and varicellazoster viruses and versus complement fixation for serodiagnosis of infections with those viruses. J. Clin. Microbiol, 6: 869-874, 1985.
4. DAVIS Bernard, - 2ª edição, Tradução de R. A. A. Moura. S.P. Harper e Row. Natureza do vírus.V.4, p. 1258-1261. 1980.
5. HANKINS, R.W & BLACK, EL - Western blot ana1yses of measles virus antibody in normal persons and in patients with multiple sc1erosis subacute. Clin Microbiol. 9: 324 - 329, 1986.
6. LOWRY, O.H.; ROSEMBROUGH, H.J.; FARR, L& RANDALL, R. - Protein meaurement with the Folin plenol reagent. J. Biol. Chem. 193: 263-75, 1951.
7. NORRBY, E. - Hemagglutination by measles virus IV. A simple procedure for production of righ potency antigen for hemaglutination-inhibition (RI) tests. Prac. Soc. Exp. Biol. Med. 11:814-818, 1992.
8. NORRBY, E. - Hemagglutination by measles virus III. Identification of two different hernagglutinins. Virology. 19:147 - 157, 1963.
9. PANNUTTI, C.S.; MORAES, 1.C.; SOUZA, Y.A.Y.E, CAMARGO, M.C.C.; HIDALGO, M.T.R. & Others. - Measles antibody prevalence after mass immunization in S.Paulo, Brasil, Bull Who. 69 (5): 557-560, 1991.
10. ROSEN, L. - Hemagglutination and hemagglutioninibition with measles vírus. Virology 13: 139-41, 1961.
11. ROTA, 1.S.; HUMMEL, K.B.; ROTA, P. & BELLINI, W. J. - Genetic variability of the gycoprotein genes of current wild-type meas1es isolates. Virology. 188: 135 - 142, 1992.
12. VOLLER, A. & BIDWELL, D.E. - Enzymeimmunoassay for antibodies in measles, cytomegalovirus infection and after rubelIa vaccination.Br. 1.Exp. Panthol., 57:243-7,1976.
13. UDEM, S.A. -Measles virus: Conditions for the propagation and purification of infectious vírus in high yield. J. Virological Methods. 8: 123-136, 1984.
This work is licensed under a Creative Commons Attribution 4.0 International License.
Copyright (c) 1995 Instituto Adolfo Lutz Journal
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