Confirmatory tests for trichothecenes
Vol. 56 No. 2 (1996), ORIGINAL ARTICLE
Vol. 56 No. 2 (1996)

Confirmatory tests for trichothecenes

ORIGINAL ARTICLE
https://doi.org/10.53393/rial.1996.56.36597
Published 1996-12-30
Maria Angélica Marochi
Bolsista da CAPES
Lucia M. Valente Soares
Faculdade de Engenharia de Alimentos-Campinas-SP
Regina P. Z. Furlani
Faculdade de Engenharia de Alimentos-Campinas-SP
pdf (Português (Brasil))

Keywords

Mycotoxins
trichothecenes
thin layer chromatography
confírmatory tests for trichothecenes

How to Cite

1.
Angélica Marochi M, M. Valente Soares L, P. Z. Furlani R. Confirmatory tests for trichothecenes. Rev Inst Adolfo Lutz [Internet]. 1996 Dec. 30 [cited 2024 May 20];56(2):17-20. Available from: https://periodicos.saude.sp.gov.br/RIAL/article/view/36597
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Abstract

Tcsts are described for confirmation of the identity oftrichothecenes (deoxynivalenol, diacetoxycispemol, toxinT-2, roridin A, and vermcarinA) on thin layer chromatrography using(a) anacetylation reaction cmployed previous to a chromatographic development and(b) 5 spray reagents (sulfuricacid,acid, 
ceric sulfate, 2,4 dinitrophenylhydrazine, p-anisaldehyde, chrornotropic acid) employedafter a chromatographic development. Nivalenoldid notrespondto the spray reagents being utilized and thus could not be included in the present proposal. The system was tested on eight samples of corn previously innoculated and incubated with Fusarium trichothecene producing strains. Diacetoxyscirpenol and T-2 toxin were obtained and confirmedby the proposedtests. Theresultswhen comparedwiththeonesobtainedwithmassspectrometrydata,demonstrate the usefulness and ease of applicationof the systemfor confirmation ofthe identityof the abovementioned trichothecenesonthin layerchromatography.

https://doi.org/10.53393/rial.1996.56.36597
pdf (Português (Brasil))

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Copyright (c) 1996 Instituto Adolfo Lutz Journal

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