Keywords
Microscopy
food
light filth
dried soups
insect fragments
food
light filth
dried soups
insect fragments
How to Cite
1.
Helena Martini M, Paschoal Batistuti J. Light filth in dried foods - Microscopic method evaluation. Rev Inst Adolfo Lutz [Internet]. 1998 Dec. 30 [cited 2025 Apr. 2];57(2):35-44. Available from: https://periodicos.saude.sp.gov.br/RIAL/article/view/36635
Abstract
The officia1 methodology proposed for light filth by AOAC 980.27, from barley.oatmeal, and mixed dry infant cereals, was tested for dried soups. Soup samples were artificialy contamined with insects fragments: heads, torax-abdomen, elytral squares, mites and rodents hairs fragments. The two solvents effects were analysed, 40% isopropanol and 60% ethanol and mixtures of them I: 1,3: 1, to deffating and flotation of dried soups samples. Average recoveries of insect fragrnents, mites and rodent hairs were 92,6%, 43% and 53%, respectively for the proposed method (40% isopropanol and 60% ethanol, 3:1). These results were not significantly different (p<0,05) than the official method 980.27 that were found an of average recoveries of 93,3% for insect fragments, 39% for mites, and 61% for rodent hairs. The modifiedAOAC 980.27 method (40% isopropanol and 60% ethanol, 3: 1) was more efficient for recovery of insect fragments.The light filth evaluation in commercial dried soups with the proposed method was carried out in 62 samples (of two differents brands, A and B), divided into two lots each one with 31 samples. The dried soups were produced in the State of São Paulo and bought in Araraquara (SP) supermarkets. All of the samples were analysed in duplicates. The high incidence of light filth (nº/50g) was 81,8% (1 to 36 insects fragments) for insects fragments in brand A and 82,5% (1 to 49 insects fragments) in brand B. There was a low incidence of mites and rodent hairs fragments. These results also suggest that is need a review about an acceptable leveI for light filth in Brazilian foods legislation.
References
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20. FREEMAN, C.e. Technique for preparing spike elements for study samples of extraneous materials, J. Assoe. Off. AnaI. Chem., v.65, p.277, 1982.
21. GECAN, J.S. & ATKINSON, J. Microanalytical quality of macaroni and noodles. J. of Food Prot., vA8, p. 400-2, 1985.
22. GECAN, J.S. &ATKINSON, J. Microanalytical quality of wheat flour. J. of Food Prot., v. 46, p.582-4, 1983.
23. GECAN, J. S. & CICHOWICZ, S. M. Extraneous materials: isolation. ln: ASSOCIATION OF OFFICIAL ANALYTICAL CHEMISTS. Official methods of analysis. 14. ed. Arlington, VA., 1984. p.902.
24. GLAZE, L.E. Extraction of light fi1th from oriental fish products containin spice: collaborative study, J. Assoe.Off. AnaI. Chem., v.76, pA4- 6, 1993.
25. GLAZE, L.E. Extraction of light fi1th paste and sauce (Bagoong) not containig spice: collaborative study. J. Assoe.Off. AnaI. Chem., v.75, p. 263- 5, 1992.
26. GORHAM, J. R. Filth in foods implications for health. In: .Principles of food analysis for filth, deeomposition and foreign matter. 2. ed. Washington, DC: FDA,1981. p.27-31. (FDA Technical Bulletin, 1).
27. MAIS alimentos - Gazeta mercantil, São Paulo, 07 fev 1996a, caderno Opinião p. A- 3.
28. NICHOLSON, J.F., HARRrS, K.L.& YAKOWITZ, M.G. Principles of isolation and detection of food and drug contaminants. ln: HARRIS, K.L.& REYNOLDS, H.L.(Ed.) Microseopie analytieal methods in food and drug eontrol. Washington, DC: FDA, 1960. p. 29- 52 (FDA Technical Bulletin, 1).
29. NAKASHIMA, M. J. Extraction of light fi1th from dried bean curd: collaborative study, J. Assoe. Off. AnaI. Chem., v.75, p.266-8, 1992.
30. OLSEN, A.R. Mites and other filth in dried shrimp imported into the United States from the Orient. J. ofFood Prot., vA5, p.1204-7, 1982.
31. REAL muda hábitos de consumo. Gazeta Mercantil, São Paulo, 07 fev. 1996b, caderno Agribusiness, p B-16.
32. SHOSTAK, M.T. lmproved method for determination of 1ight fi1th in chocolate products. J. Assoe. Off. AnaI. Chem., v.61, p.996- 8, 1978.
33. VAZQUEZ, A.W. Recognition of insect fragments. ln: GORHAM, J.R. Training manual for analytical entomology in the food industry. Washington, DC: FDA, 1978. p.- 52,61-75. (FDA Technical Bulletin 2).
34. ZAMBONI, C. Q. Curso de Bromatologia para Técnicos do Ministério da Saúde. Aulas teóricas de microscopia alimentar. São Paulo: Instituto Adolfo Lutz,1977. 105 p. (mimeog.).
35. ZAMBONI, C. Q. & ATUI, M.B. Comparação entre métodos para pesquisa de sujidades e verificação das condições de higiene das massas alimentícias por microscopia. Rev. Inst. Adolfo Lutz, v. 49, p.11- 7, 1989.
36. ZAMBONI, C. Q., ALVES, H.I. &ATUI, M.B. Métodos para detecção de sujidades leves em sopas desidratadas. Rev. Inst. Adolfo Lutz, v.50, p.30l- 5, 1990.
37. ZAMBONI, C. Q., ALVES, H.I. , RODRIGUES, R. M. M. S., SPITERI, N., ATUI, M. B. & SANTOS, M. C. Fraudes e sujidades em condimentos comercializados na cidade de São Paulo. Rev. Inst. Adolfo Lutz, v.51, p.19- 22, 1991.
38. ZAMBONI, C. Q., RODRIGUES, R. M. M. S., SPITERI, N., ALVES, H.I. , BATISTIC, M. A., ATUI, M.B. & SANTOS, M. C. Fraudes e condições de higiene de doces em pasta. Rev. Inst. Adolfo Lutz, v.49, p.125-9, 1989.
39. ZAMBONI, C. Q., ALVES, H.I., RODRIGUES, R. M. M. S., SPITERI, N., ATUI, M. B. & BATISTIC, M. A. Sujidades e fraudes em chocolates. Rev. Inst. Adolfo Lutz, v.48, p.37- 41, 1988.
2. BARBIERI, M. K. Matérias estranhas: contaminação, técnicas de isolamento e detecção em farinha de trigo. Colet. ITAL, (Campinas), v. 22 , p.13- 22, 1992.
3. BARBIERI, M. K. Microscopia em alimentos: identificação histológica, isolamento e detecção de material estranho em alimentos. Campinas: ITAL, 1990. 109p. (Manual Técnico).
4. BOESE, J. & BANDLER, R. M. Extraneous materials: isolation. In: ASSOCIATION OF OFFICIAL ANALYTICAL CHEMISTS. Official methods of analysis. 15.ed. Arlington, VA., 1990. v.l, p.369406.
5. BOESE, J., CICHOWICZ & S. M. Extraneous materials: isolation. In: ASSOCIATION OF OFFICIAL ANALYTICAL CHEMISTS. Official methods of analysis. 16.ed. Arlington, VA., 1995. v.1, p.1- 47.
6. BRASIL. Portaria nº 74, 4 ago. 1994. Estabelece o limite máximo de 75 fragmentos de insetos em 50g de farinha de trigo e 225 fragmentos em 225g para massas alimentícias, biscoitos, produtos de panificação e confeitaria. Diário Oficial, Brasília, p.11809.
7. BRASIL. Resolução Normativa nº 12/78. Aprova Normas Técnicas Especiais do Estado de São Paulo, relativa a alimentos e bebidas. Diário Oficial, Brasília 24 de jul. 1978, Seção r, pt. r, p.11525.
8. BRICKEY JR., P. M. Concepts of food protection food sanitation and FD&C Act. ln: GORHAM, J.R. PrincipIes of food analysis for filth, deeomposition and foreign matter. 2. ed. Washington, DC: FDA, 1981, p.3-10.
9. BRICKEY JR., P. M. Identification of insects fragments. In: GORHAM, J.R. Training manual for analytical entomology in food industry. Washington, DC, FDA, 1978, p. 53- 60 (FDA Technical Bulletin, 2).
10. BRICKEY JR, P.M., GECAN, J.S., THRASHER J.J.& EISENBERG, W. V. Notes on microanalytical techniques in the analysis of foods for extraneous materials. J. Assoe.Off. AnaI. Chem., v.51, p.8726, 1968.
11. BRrCKEY JR, P.M., GECAN, J.S., THRASHER J.J.& VAZQUEZ, A.W.Notes on microanalytical techniques in the analysis of foods for extraneous materials. J. Assoe.Off. AnaI. Chem, Washington, v.55, p.51- 6, 1972.
12. CANO, e. B., ZAMBONr, e. Q., ALVES, H. 1., SPITERI, N., ATUr, M. B., SANTOS, M. c., JORGE, L. I. F., PEREIRA, U. & RODRIGUES, R. M.M. Mel: fraudes e condições sanitárias. Rev. Inst. Adolfo Lutz, v.52, p.1- 4, 1992.
13. CORRErA, M. &ATUI, M. B. Metodologia de análise microscópica para avaliação das condições higiênicas em misturas para o preparo de sopas e risotos. Rev. Inst. Adolfo Lutz, v.55, p.73- 7, 1995.
14. DECANrO. M. G. Infestação de produtos alimentícios nas fontes de produção e durante o armazenamento. Um método para a pesquisa microscópica de sujidades e impurezas. Rev. Inst. Adolfo Lutz, v.3, p.131- 7, 1971.
15. DE CESARE. Crescimento em ritmo de fast- food. Superhiper, São Paulo, p. 156, 158, out. 1995.
16. DENT, R.G. Extraction of light fi1th from barley, oat-meal, and mixed dry infant cereals: collaborative study. J. Assoe. Off. AnaI. Chem., v.63, p.187- 8, 1980.
17. DENT, R.G. & GLAZE,L. Extraction of light fi1th from unground marjoram: collaboretive study. J. Assoe.Off. AnaI. Chern., v.68, p.899-901, 1985.
18. EISENBERG, W. V. Source of food contaminants. In: GORHAM, J.R. PrincipIes of food analysis for filth, deeomposition and foreign matter. 2. ed. Washington, DC: FDA,1981. p.l1- 26.(FDA Technical Bulletin, 1).
19. FREEMAN, e.C. Brine saturation technique for extraction of ligth fi1th from rubbed, ground, and whole sage: collaborative study, J.Assoe.Off. AnaI. Chem., v.68, p.894- 8, 1985.
20. FREEMAN, C.e. Technique for preparing spike elements for study samples of extraneous materials, J. Assoe. Off. AnaI. Chem., v.65, p.277, 1982.
21. GECAN, J.S. & ATKINSON, J. Microanalytical quality of macaroni and noodles. J. of Food Prot., vA8, p. 400-2, 1985.
22. GECAN, J.S. &ATKINSON, J. Microanalytical quality of wheat flour. J. of Food Prot., v. 46, p.582-4, 1983.
23. GECAN, J. S. & CICHOWICZ, S. M. Extraneous materials: isolation. ln: ASSOCIATION OF OFFICIAL ANALYTICAL CHEMISTS. Official methods of analysis. 14. ed. Arlington, VA., 1984. p.902.
24. GLAZE, L.E. Extraction of light fi1th from oriental fish products containin spice: collaborative study, J. Assoe.Off. AnaI. Chem., v.76, pA4- 6, 1993.
25. GLAZE, L.E. Extraction of light fi1th paste and sauce (Bagoong) not containig spice: collaborative study. J. Assoe.Off. AnaI. Chem., v.75, p. 263- 5, 1992.
26. GORHAM, J. R. Filth in foods implications for health. In: .Principles of food analysis for filth, deeomposition and foreign matter. 2. ed. Washington, DC: FDA,1981. p.27-31. (FDA Technical Bulletin, 1).
27. MAIS alimentos - Gazeta mercantil, São Paulo, 07 fev 1996a, caderno Opinião p. A- 3.
28. NICHOLSON, J.F., HARRrS, K.L.& YAKOWITZ, M.G. Principles of isolation and detection of food and drug contaminants. ln: HARRIS, K.L.& REYNOLDS, H.L.(Ed.) Microseopie analytieal methods in food and drug eontrol. Washington, DC: FDA, 1960. p. 29- 52 (FDA Technical Bulletin, 1).
29. NAKASHIMA, M. J. Extraction of light fi1th from dried bean curd: collaborative study, J. Assoe. Off. AnaI. Chem., v.75, p.266-8, 1992.
30. OLSEN, A.R. Mites and other filth in dried shrimp imported into the United States from the Orient. J. ofFood Prot., vA5, p.1204-7, 1982.
31. REAL muda hábitos de consumo. Gazeta Mercantil, São Paulo, 07 fev. 1996b, caderno Agribusiness, p B-16.
32. SHOSTAK, M.T. lmproved method for determination of 1ight fi1th in chocolate products. J. Assoe. Off. AnaI. Chem., v.61, p.996- 8, 1978.
33. VAZQUEZ, A.W. Recognition of insect fragments. ln: GORHAM, J.R. Training manual for analytical entomology in the food industry. Washington, DC: FDA, 1978. p.- 52,61-75. (FDA Technical Bulletin 2).
34. ZAMBONI, C. Q. Curso de Bromatologia para Técnicos do Ministério da Saúde. Aulas teóricas de microscopia alimentar. São Paulo: Instituto Adolfo Lutz,1977. 105 p. (mimeog.).
35. ZAMBONI, C. Q. & ATUI, M.B. Comparação entre métodos para pesquisa de sujidades e verificação das condições de higiene das massas alimentícias por microscopia. Rev. Inst. Adolfo Lutz, v. 49, p.11- 7, 1989.
36. ZAMBONI, C. Q., ALVES, H.I. &ATUI, M.B. Métodos para detecção de sujidades leves em sopas desidratadas. Rev. Inst. Adolfo Lutz, v.50, p.30l- 5, 1990.
37. ZAMBONI, C. Q., ALVES, H.I. , RODRIGUES, R. M. M. S., SPITERI, N., ATUI, M. B. & SANTOS, M. C. Fraudes e sujidades em condimentos comercializados na cidade de São Paulo. Rev. Inst. Adolfo Lutz, v.51, p.19- 22, 1991.
38. ZAMBONI, C. Q., RODRIGUES, R. M. M. S., SPITERI, N., ALVES, H.I. , BATISTIC, M. A., ATUI, M.B. & SANTOS, M. C. Fraudes e condições de higiene de doces em pasta. Rev. Inst. Adolfo Lutz, v.49, p.125-9, 1989.
39. ZAMBONI, C. Q., ALVES, H.I., RODRIGUES, R. M. M. S., SPITERI, N., ATUI, M. B. & BATISTIC, M. A. Sujidades e fraudes em chocolates. Rev. Inst. Adolfo Lutz, v.48, p.37- 41, 1988.
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Copyright (c) 1998 Instituto Adolfo Lutz Journal
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