Keywords
virus-like particle
R-type virus-like particle
detection
structure
cell
BHK-21
culture
electron microscopy
R-type virus-like particle
detection
structure
cell
BHK-21
culture
electron microscopy
How to Cite
1.
Ueda M, Tanaka H, Joazeiro DR, Weigl PP. Viral particles in BHK-21 cell culture: ultrastructure, occurrence and meaning. Rev Inst Adolfo Lutz [Internet]. 1982 Dec. 30 [cited 2025 Jan. 10];42(1-2):71-5. Available from: https://periodicos.saude.sp.gov.br/RIAL/article/view/36783
Abstract
Electron microscopy of cul tures of BHK-21 cells disclosed vírus-Iike particles resembling the "R" particles observed by other authors. Particles seemingly originated th rough budding of the endoplasmatic reticulum membrane. Fully grown particles were usually found in the lumen of the endoplasmic reticulum 0r in the perinuclear space.
References
1. BERGMANN, D.G. & WOLFF, D.A. Production of R-type virus-like particles in hamster cells. lntervirology, 16 :61-70, 1981.
2. BERNHARD, W. & TOURNIER, P. - Infection virale inapparente de cellules de hamsters décelée par Ia mieroscopie électtronique. Ann. Inst. Paeteur, Paris, 107: 447-51, 1964.
3. CROMACK, A.S. - An electron microscopc study of virus-like particles in chiek embryo and L cells cultures. J. gen. virol., 2: 195-8, 1968.
4. DE HARVEN, E. - ldentification of tissue culture contaminants by electron microscopy. In : FOGH, J., cd. - Contamination in iieeue culture. N ew York, Academic Press, 1973. p. 205-31.
5. MURPHY, F.A.; HALONEN, P.E. & HARRISON, A.R. - Electron microscopy of development of Rubella virus in BHK-21 cells. J. Viro I, 2: 1223-7, 1968.
6. MUSSGAY, M.; RECZKO, E. & AHL, R. Demonstration of virus-like particles in a bovine cell line. J. gen. Viro I., 4 :445-7, 1969.
7. REYNOLDS, E.S. - The use of lead citrate at high p H as an electron-opaque stain in electron microscopy. J. Cell. Biol., 17: 208-12, 1963.
8. ROSSlGNOL, J.M.; KRESS, M. & RECONDO, A.M. - Reverse transcriptase activity associated with R-type virus-like particles of SV 40 - transformed hamster cells (TSV. clone 2). Intervirology, 5: 273-92, 1975.
9. SHIPMAN, C.J.; VANDER WEIDE, G.C. & ILMA, B. - Prevalence of type R vírus-Iike particles in clories of BHK-21 cells. Virology, 38 :707-10, 1969.
10. WElGL, D.R. & KlSlELIUS, J.J. - A resina Polylite na microscopia eletrônica. Cienc. Cult., São Paulo, 24 (6, supl.) : 212, 1972.
2. BERNHARD, W. & TOURNIER, P. - Infection virale inapparente de cellules de hamsters décelée par Ia mieroscopie électtronique. Ann. Inst. Paeteur, Paris, 107: 447-51, 1964.
3. CROMACK, A.S. - An electron microscopc study of virus-like particles in chiek embryo and L cells cultures. J. gen. virol., 2: 195-8, 1968.
4. DE HARVEN, E. - ldentification of tissue culture contaminants by electron microscopy. In : FOGH, J., cd. - Contamination in iieeue culture. N ew York, Academic Press, 1973. p. 205-31.
5. MURPHY, F.A.; HALONEN, P.E. & HARRISON, A.R. - Electron microscopy of development of Rubella virus in BHK-21 cells. J. Viro I, 2: 1223-7, 1968.
6. MUSSGAY, M.; RECZKO, E. & AHL, R. Demonstration of virus-like particles in a bovine cell line. J. gen. Viro I., 4 :445-7, 1969.
7. REYNOLDS, E.S. - The use of lead citrate at high p H as an electron-opaque stain in electron microscopy. J. Cell. Biol., 17: 208-12, 1963.
8. ROSSlGNOL, J.M.; KRESS, M. & RECONDO, A.M. - Reverse transcriptase activity associated with R-type virus-like particles of SV 40 - transformed hamster cells (TSV. clone 2). Intervirology, 5: 273-92, 1975.
9. SHIPMAN, C.J.; VANDER WEIDE, G.C. & ILMA, B. - Prevalence of type R vírus-Iike particles in clories of BHK-21 cells. Virology, 38 :707-10, 1969.
10. WElGL, D.R. & KlSlELIUS, J.J. - A resina Polylite na microscopia eletrônica. Cienc. Cult., São Paulo, 24 (6, supl.) : 212, 1972.
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Copyright (c) 1982 Instituto Adolfo Lutz Journal
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