Abstract
It was described ;n this work a simple method for separatíon and identification of choline chloride and Betaíne hydrochloride, when mixed ín pharmaceutical pre-
paration, using chromatography technique in ascendent papel'. The Rf of both substances have been established, whether when mixed wíth pharmaceutícal products whether separated in a simple water solution. Satisfactory results are got in separation and identification of Betaíne and
Cholíne in above conditions.
References
1. AMERICAN PHARMACEUTICAL ASSOCIATION FOUNDATI'ON - Qualitative and quantitative tests for chlor'at betaine. J. Pharm. Sec., 53 :1385-6, 1964.
2. APPLETON, H. D.; LA DUE JR., B. N.; LEVY, B. B.; STEELE, J. M. ,& BRODIE, B. B. - A chemical method for the deterrnínatíon of free choline in plasma. J. Biol. cnem., 205:803-13, 1953.
3. BREGOFF, H. M.; ROBERTS, E. & DELWICHE, C. C. - Papel' chrornatography or quaternary ammoníum bases and related compounds. J. Biol. Oh.em., 205:565-74, 1953.
4. CHRISTIANSON, D. D.; WALL, J. S.; CAVINS, J. F. & DIMLER, R. .T. - Chromatography of quaternary nitrogen compounds on buffered eatíon-exchange resins. J. Ohromatogr., 10:432-8, 1963.
5). HRDY, O. & LOCHMANOVÁ, S. - Determination of choline and betaine in the presence or each other. Ceskosl, Farm., 9(7) :335-9, 1960, apud Analyt. Abstr., 8: 1164, 1961.
6. JANOT, M. M. ,& LEHIR, A. - (Ihronuitographie 8ur papier. In LEDERER, E. - ChromatographiB en chimiB organic et biolo qiqwe, Paris, Masson, 1959. v_ 1, p.511.
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8. SANDRI, G. - Bromathallic acid as a new reagent for the identification af organic bases. Mikrochem. A.cta, 2:253-8, 1958.
9. SPEED, n. ,& RICHARDSON, M. ~- Chromatographic methods for the isolation and identification of the products of choline oxidation. J. cnrom atoçr., 35:497~505, 1968.
10. WALKER .IR., H. G. <& ERLANDSEN, R. Rapid method for determination or betaíne. Analyt. Ch.em., 23(9) :1309-11, 1951.
11. WALL,. J. S.; CHRISTIANSON, D. D.; DIM~ LER, R. J_ ,& SENTI, F. R. - Spectro-photometric determination of betaines aand other quaternary nitrogen compounds as therr periodides. Analyt. Chem., 32 (7) :870-4, 1960.
2. APPLETON, H. D.; LA DUE JR., B. N.; LEVY, B. B.; STEELE, J. M. ,& BRODIE, B. B. - A chemical method for the deterrnínatíon of free choline in plasma. J. Biol. cnem., 205:803-13, 1953.
3. BREGOFF, H. M.; ROBERTS, E. & DELWICHE, C. C. - Papel' chrornatography or quaternary ammoníum bases and related compounds. J. Biol. Oh.em., 205:565-74, 1953.
4. CHRISTIANSON, D. D.; WALL, J. S.; CAVINS, J. F. & DIMLER, R. .T. - Chromatography of quaternary nitrogen compounds on buffered eatíon-exchange resins. J. Ohromatogr., 10:432-8, 1963.
5). HRDY, O. & LOCHMANOVÁ, S. - Determination of choline and betaine in the presence or each other. Ceskosl, Farm., 9(7) :335-9, 1960, apud Analyt. Abstr., 8: 1164, 1961.
6. JANOT, M. M. ,& LEHIR, A. - (Ihronuitographie 8ur papier. In LEDERER, E. - ChromatographiB en chimiB organic et biolo qiqwe, Paris, Masson, 1959. v_ 1, p.511.
7. NIEMANN, A. - Zu r Trennung von Betain und Chol ín an ionenaustauschern. .T. Chromatogr., 9:117-8, 1962.
8. SANDRI, G. - Bromathallic acid as a new reagent for the identification af organic bases. Mikrochem. A.cta, 2:253-8, 1958.
9. SPEED, n. ,& RICHARDSON, M. ~- Chromatographic methods for the isolation and identification of the products of choline oxidation. J. cnrom atoçr., 35:497~505, 1968.
10. WALKER .IR., H. G. <& ERLANDSEN, R. Rapid method for determination or betaíne. Analyt. Ch.em., 23(9) :1309-11, 1951.
11. WALL,. J. S.; CHRISTIANSON, D. D.; DIM~ LER, R. J_ ,& SENTI, F. R. - Spectro-photometric determination of betaines aand other quaternary nitrogen compounds as therr periodides. Analyt. Chem., 32 (7) :870-4, 1960.
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Copyright (c) 1971 Instituto Adolfo Lutz Journal
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