Keywords
sorbitol, identification and separation
thín-layer chromatography, identification and separation of sorbitol
thín-layer chromatography, identification and separation of sorbitol
How to Cite
1.
Lara WH, Yabiku HY. Thin-layer chromatography method for identification of sorbitol. Rev Inst Adolfo Lutz [Internet]. 1974 Dec. 30 [cited 2024 Dec. 26];34(1-2):79-81. Available from: https://periodicos.saude.sp.gov.br/RIAL/article/view/37060
Abstract
A thín-Iayer chromatography method for identification and separation of sorbítol from its contaminants (mannitol, maltose, glucose, xylose, fructose, sucrose) is described. The solvent used ís a mixture of water, propanol and ethyl acetate and the spray reagent is a solution of potassium permanganate. Method detects up to 0.5 ug/ III of sorbitol and clearly separates up to 5% of mannitol from sorbitol.
References
1. BLACKLEY, R.L. - The metabolism and antiketogenic effects of sorbitol. Sorbitol dehydrogenase. Biochem. J., 49: 257-71, 1951.
2. BRASIL. Leis, decretos etc. - Decreto-lei n. O 55.871 de 26 de março de 1965. Modifica o Decreto n.? 50.040 de 24 de janeiro de 1961, referente a normas reguladoras do emprego de aditivos para
alimentos, alterado pelo Decreto n.o 691 de 13 de março de 1962. Diário Oficial da União, Brasília,DF, 9 abro 1955.
3. COLES JR., Z.A. & UPTON, R.P. - Thinlayer chromatographic identification test for sorbitol and sorbitol solution. J. Ass. offic. analyt. Chem., 55: 1004-5, 1972.
4. DE SIMONE, V. & VICEDONIMI, M. - The separatíon of polyols by thin-layer chromatography. J. Chromat., 37: 538-41, 1968.
5. EGAN, H. & COLLINS, H.J. - Survey of analytícaí methods available for the estímatron of some food addrtíves in food. Pure appl. Chem., 26: 113-4, 1971.
6. HAUSE, J.A.; HUBICKI, J.A. & HAZER, G. G. - Determination of sorbitol as its hexacetate by gas liquid chromatography using an ionization detectar. Analyt. Chem., 34: 1567-70, 1962.
7. GENEST, C. & CHAPMAN, D.G. - Extractton and identification of sugar alcohols and other carbohydrates in dietetic foods. J. Ass. offic. agric. Anal., 45: 422-4, 1963.
8. PHARMACOPEIA of the United States of Amertca , 18th rev. [Easton, Pa., Mack Printing, c1970. p. 678-680.
9. TICKHOMIRA, G.P. & KALITY VAÚSKA, V. r. Thin-Iayer chromatographic analysis of sorbitol-manitol mixtures in the product obtained by hydrogenation of glucose for the manufature of sorbítolo Ukr. Khim. zn.. 38 (11): 1166-8, 1972 apud Analyt. Abstr . 25 (3): 1921, 1973.
2. BRASIL. Leis, decretos etc. - Decreto-lei n. O 55.871 de 26 de março de 1965. Modifica o Decreto n.? 50.040 de 24 de janeiro de 1961, referente a normas reguladoras do emprego de aditivos para
alimentos, alterado pelo Decreto n.o 691 de 13 de março de 1962. Diário Oficial da União, Brasília,DF, 9 abro 1955.
3. COLES JR., Z.A. & UPTON, R.P. - Thinlayer chromatographic identification test for sorbitol and sorbitol solution. J. Ass. offic. analyt. Chem., 55: 1004-5, 1972.
4. DE SIMONE, V. & VICEDONIMI, M. - The separatíon of polyols by thin-layer chromatography. J. Chromat., 37: 538-41, 1968.
5. EGAN, H. & COLLINS, H.J. - Survey of analytícaí methods available for the estímatron of some food addrtíves in food. Pure appl. Chem., 26: 113-4, 1971.
6. HAUSE, J.A.; HUBICKI, J.A. & HAZER, G. G. - Determination of sorbitol as its hexacetate by gas liquid chromatography using an ionization detectar. Analyt. Chem., 34: 1567-70, 1962.
7. GENEST, C. & CHAPMAN, D.G. - Extractton and identification of sugar alcohols and other carbohydrates in dietetic foods. J. Ass. offic. agric. Anal., 45: 422-4, 1963.
8. PHARMACOPEIA of the United States of Amertca , 18th rev. [Easton, Pa., Mack Printing, c1970. p. 678-680.
9. TICKHOMIRA, G.P. & KALITY VAÚSKA, V. r. Thin-Iayer chromatographic analysis of sorbitol-manitol mixtures in the product obtained by hydrogenation of glucose for the manufature of sorbítolo Ukr. Khim. zn.. 38 (11): 1166-8, 1972 apud Analyt. Abstr . 25 (3): 1921, 1973.
This work is licensed under a Creative Commons Attribution 4.0 International License.
Copyright (c) 1974 Instituto Adolfo Lutz Journal
Downloads
Download data is not yet available.