Resultados da razão sinal da amostra sobre o cutoff da reação no ensaio Abbott Architect rHTLV-I/II permite prever a detecção de DNA proviral por PCR em tempo real
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Palavras-chave

Infecções por HTLV-1
Infecções por HTLV-2
Imunoensaio Quimioluminescente de Micropartículas (CMIA)
Reação em Cadeia da Polimerase em Tempo Real
Doadores de Sangue

Como Citar

1.
Cruz LJ do N, Maneschy C de A, Barile KA do S, Palmeira MK, Amaral CE de M. Resultados da razão sinal da amostra sobre o cutoff da reação no ensaio Abbott Architect rHTLV-I/II permite prever a detecção de DNA proviral por PCR em tempo real. Rev Inst Adolfo Lutz [Internet]. 24º de agosto de 2022 [citado 29º de março de 2024];81:1-11,e37345. Disponível em: https://periodicos.saude.sp.gov.br/RIAL/article/view/37345

Resumo

O estudo tem como objetivo correlacionar às distribuições das razões sample-to-cutoff (S/CO) de resultados reagentes para anticorpos HTLV-1/2 com a detecção de DNA proviral em uma população de candidatos à doação de sangue. Realizou-se uma busca retrospectiva de dados de 632 amostras reagentes para HTLV-1/2 submetidas à testagem confirmatória entre janeiro de 2015 a dezembro de 2019. A triagem sorológica foi realizada pelo imunoensaio quimioluminescente de micropartículas Architect rHTLV-I/II, enquanto o teste confirmatório foi realizado pelo método de PCR em tempo real in-house. 496 de 632 amostras (78%) apresentaram DNA proviral indetectável e 136 (22%) apresentaram DNA proviral detectável. A infecção por HTLV não foi confirmada em nenhum indivíduo com valor de S/CO <4 e as taxas de detecção de DNA proviral escalonaram gradualmente à medida que as razões S/CO aumentaram. A sensibilidade e valor preditivo positivo encontrados para o Architect rHTLV-I/II foram 100% e 22%, respectivamente. Utilizando análise de curva ROC, o valor de razão S/CO ideal para predizer a presença de DNA proviral foi de 18,11. Razões S/CO elevadas foram mais associadas à detecção de DNA proviral. Em suma, o valor de S/CO <4 sugere a exclusão de infecção por HTLV e o risco de transmissão pelo sangue.

https://doi.org/10.53393/rial.2022.v81.37345
pdf (English)

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Copyright (c) 2022 Lucas José do Nascimento Cruz, Carolina de Alcântara Maneschy, Katarine Antonia do Santos Barile, Maurício Koury Palmeira, Carlos Eduardo de Melo Amaral

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