Keywords
T-2 toxin
trichothecene determination
TLC
How to Cite
Abstract
The trichothecenes are a group of sesquiterpenoid mycotoxins produced by several general and species of fungi such as Fusarium, Stachybotris, Trichothecium, Trichoderma and Myrothecium. These trichothecenes are relatively stable during food processing and can be removed with difficulty from contamined grains under moderate conditions. Among then deoxynivalenol (DON) is the most
common contaminant of cereal grains, whilst the T-2 toxin, wich is frequently detected in foods, has been the most widely, because is 10 times more toxic then DON. In the present work it was adapted a methodology for simultaneous determination of DON and T-2 toxin by thin layer chromatography (TLC). The matrix
chosen was barley malt. Extraction and purification systems, developing solvents and spray reagents
used to visualize the trichothecenes have been compared. The method was employed to rice and wheat meals. The limits of detection of the method were 40 ng/spot for T-2 toxin and 50 ng/spot; with the average recoveries of 77,9 and 80,5% respectively. A rice sample was found contamined with 266 µg. Kg-1 and two samples of wheat meal with 128 e 323 µg. Kg-1 DON.
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