Palabras clave
Isolation and growth
Arbovirus
Cell line
Aedes albopictus, clone C6/36
Arbovirus
Cell line
Aedes albopictus, clone C6/36
Cómo citar
1.
Barbosa ML, Rocco lray M, Felippe JMMS, Cruz AS. Growth and Maintenance of Aedes albopictus Cell line, clone C6/36, in Diferrent Media. Rev Inst Adolfo Lutz [Internet]. 30 de diciembre de 1993 [citado 26 de diciembre de 2024];53(1-2):63-70. Disponible en: https://periodicos.saude.sp.gov.br/RIAL/article/view/36056
Resumen
The sensitivity of Aedes albopicts cell line, clone C6/36, to arbovirus isolation and growth has been being shown by several authors. The present work has verified which, among several media under the same conditions, would be the most efficient one for C6/36 cultivation and viral isolation. The L-15 medium has proved to be the best among others (MEM, DMEM and 199) with the quickest viral isolation (DEN-1). Also, in this medium, the samples could be observed until the 14th day, without significative cellular death. The results reccommend L-15 medium as the most efficíent and economic one for the purposes.
Citas
1. CHAPPELL W.A., CAUSHER C.H., TOOLE R.F., MANESS K.C., SASSO D.R. & HENDERSON RE., (1971). Comparation of three methods used to isolate Dengue virus type 2. Applied Microbiology, 22, 1100-1103.
2. CLARKE, D.H. & CASALS, J., (1958). Techniques for hemmaglutination and hemmaglutination-inhibition with arthropod-borne viruses. American Joumal of Tropical Medicine Hygiene 7, 561-573.
3. GUBLER, D.J., KUNO, G., SATHER, G.E., VELEZ, M. & OUVER, A (1984). Mosquito cell cultures and specific monoclonal antibodies in surveillance for Dengue viruses. American Journal of Tropical Medicine and Hygiene 33(1), 158-165.
4. IGARASHI, A (1978) Isolation of a Singh's Aedes albopictus cell clone sensitive to Dengue and Chikungunga viruses. Journal of General Virology 40,531-544.
5. KUBERSKI, T.T. & ROSEN, L., (1977). A simple technique for the detection of dengue antigen in mosquitoes by immunofluorescence. American Journal of Tropical Medicine and Hygiene 26, 533-537.
6. KUNO, G. (1982). Dengue virus replication in a poliploid mosquito cell culture grown in serum-free medium. Journal of Clinical Microbiology, 16 (5), 851-855.
7. KUNO, G. (1983). Cultivation of mosquito cell lines in serum-free medium and their effects on Dengue virus replication. In Vitro 19(9), 707-713.
8. KUNO, G., HlNK, W.F. & BRIGGS, J.D. (1971). Growth promoting serum proteins for Aedes albopictus cells cultured in vitro. Joumal of lnsect Physiology, 17, 1865-1879.
9. KUNO, G., MOORE, C.G. & SATHER, G.E. (1982). Factors affecting syncitial development in Aedes pseudoscutellaria cells by Dengue viruses. American Journal of Tropical Medicine and Hygiene 30( 4), 870-875.
10. KUNO, G., GUBLER, D.J., VELEZ, M. & OUVER, A (1985). Comparative sensitivity of three mosquito cell lines for isolation of Dengue viruses. Bulletin of the World Health Organization, 63 (2), 279-286.
11. PAUL, S.D., SINGH, K.R.P. & BHAT, U.K.M. (1969). A study on the cytopathic effect of arboviruses on cultures from Aedes albopictus cell line. Indian Journal of Medical Research, 57, 339-348.
12. RACE, M., WILLIAMS, M.C. & AGOSTINI, C.F. (1979). Dengue in the Caribbean: virus isolation in a mosquito (Aedes pseudoscutellaris) cell line. Transactions of the Royal Society of Tropical Medicine and Hygiene, 73, 18-22.
13. RANDOLPH, V. B. & STOLLAR, V. (1990). Low pH-induced cell fusion in flavivirus-infected Aedes albopictus cell cultures. Journal of General Virology, 71, 1845-1850.
14. RECHCIGL JR., M. (1977). CRC Handbook series in Nutrition and Food, section G: diets, food supplements, volume IV, culture media for cells, organs and embryos.
15. TESH, R.R (1979). A method for the isolation and identification of dengue viruses, using mosquito cell cultures. Journal of Tropical Medicine and Hygiene 28 (6), 1053 1059.
16. WATERMAN, S.H. & MONATH, T.P. (1982). Fluorescent antibody techniques applied to the identification of dengue virus in infected tissues. Acta Virologica, 26, 367-381.
17. WHITE, L.A., (1987). Susceptibility of Aedes albopictus C6/36 to viral infection. Journal of Clinical Microbiology, 25(7), 1221-1224.
2. CLARKE, D.H. & CASALS, J., (1958). Techniques for hemmaglutination and hemmaglutination-inhibition with arthropod-borne viruses. American Joumal of Tropical Medicine Hygiene 7, 561-573.
3. GUBLER, D.J., KUNO, G., SATHER, G.E., VELEZ, M. & OUVER, A (1984). Mosquito cell cultures and specific monoclonal antibodies in surveillance for Dengue viruses. American Journal of Tropical Medicine and Hygiene 33(1), 158-165.
4. IGARASHI, A (1978) Isolation of a Singh's Aedes albopictus cell clone sensitive to Dengue and Chikungunga viruses. Journal of General Virology 40,531-544.
5. KUBERSKI, T.T. & ROSEN, L., (1977). A simple technique for the detection of dengue antigen in mosquitoes by immunofluorescence. American Journal of Tropical Medicine and Hygiene 26, 533-537.
6. KUNO, G. (1982). Dengue virus replication in a poliploid mosquito cell culture grown in serum-free medium. Journal of Clinical Microbiology, 16 (5), 851-855.
7. KUNO, G. (1983). Cultivation of mosquito cell lines in serum-free medium and their effects on Dengue virus replication. In Vitro 19(9), 707-713.
8. KUNO, G., HlNK, W.F. & BRIGGS, J.D. (1971). Growth promoting serum proteins for Aedes albopictus cells cultured in vitro. Joumal of lnsect Physiology, 17, 1865-1879.
9. KUNO, G., MOORE, C.G. & SATHER, G.E. (1982). Factors affecting syncitial development in Aedes pseudoscutellaria cells by Dengue viruses. American Journal of Tropical Medicine and Hygiene 30( 4), 870-875.
10. KUNO, G., GUBLER, D.J., VELEZ, M. & OUVER, A (1985). Comparative sensitivity of three mosquito cell lines for isolation of Dengue viruses. Bulletin of the World Health Organization, 63 (2), 279-286.
11. PAUL, S.D., SINGH, K.R.P. & BHAT, U.K.M. (1969). A study on the cytopathic effect of arboviruses on cultures from Aedes albopictus cell line. Indian Journal of Medical Research, 57, 339-348.
12. RACE, M., WILLIAMS, M.C. & AGOSTINI, C.F. (1979). Dengue in the Caribbean: virus isolation in a mosquito (Aedes pseudoscutellaris) cell line. Transactions of the Royal Society of Tropical Medicine and Hygiene, 73, 18-22.
13. RANDOLPH, V. B. & STOLLAR, V. (1990). Low pH-induced cell fusion in flavivirus-infected Aedes albopictus cell cultures. Journal of General Virology, 71, 1845-1850.
14. RECHCIGL JR., M. (1977). CRC Handbook series in Nutrition and Food, section G: diets, food supplements, volume IV, culture media for cells, organs and embryos.
15. TESH, R.R (1979). A method for the isolation and identification of dengue viruses, using mosquito cell cultures. Journal of Tropical Medicine and Hygiene 28 (6), 1053 1059.
16. WATERMAN, S.H. & MONATH, T.P. (1982). Fluorescent antibody techniques applied to the identification of dengue virus in infected tissues. Acta Virologica, 26, 367-381.
17. WHITE, L.A., (1987). Susceptibility of Aedes albopictus C6/36 to viral infection. Journal of Clinical Microbiology, 25(7), 1221-1224.
Esta obra está bajo una licencia internacional Creative Commons Atribución 4.0.
Derechos de autor 1993 Revista del Instituto Adolfo Lutz
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