Palavras-chave
Isolamento e crescimento
Arbovírus
Linhagem de células
Aedes albopictus, clone C6/36
Arbovírus
Linhagem de células
Aedes albopictus, clone C6/36
Como Citar
1.
Barbosa ML, Rocco lray M, Felippe JMMS, Cruz AS. Crescimento e manutenção de linhagem celular Aedes albopictus clone C6/36 em diferentes meios. Rev Inst Adolfo Lutz [Internet]. 30º de dezembro de 1993 [citado 26º de dezembro de 2024];53(1-2):63-70. Disponível em: https://periodicos.saude.sp.gov.br/RIAL/article/view/36056
Resumo
A sensibilidade da linhagem de células Aedes albopictus, clone C6/36, para isolamento e crescimento de arbovírus, tem sido demonstrada por vários autores. O presente trabalho verificou, qual entre vários meios, nas mesmas condições, seria mais eficiente para cultivo de C6/36 e isolamento viral. O meio L-15 mostrou ser o melhor entre outros (MEM, DMEM e199), com mais rapidez no isolamento viral (DEN-l). Também, nesse meio, as amostras puderam ser observadas até o 14° dia, sem morte celular significativa. Os resultados recomendam o meio L-15 como o mais eficiente e econômico para os propósitos apresentados.
Referências
1. CHAPPELL W.A., CAUSHER C.H., TOOLE R.F., MANESS K.C., SASSO D.R. & HENDERSON RE., (1971). Comparation of three methods used to isolate Dengue virus type 2. Applied Microbiology, 22, 1100-1103.
2. CLARKE, D.H. & CASALS, J., (1958). Techniques for hemmaglutination and hemmaglutination-inhibition with arthropod-borne viruses. American Joumal of Tropical Medicine Hygiene 7, 561-573.
3. GUBLER, D.J., KUNO, G., SATHER, G.E., VELEZ, M. & OUVER, A (1984). Mosquito cell cultures and specific monoclonal antibodies in surveillance for Dengue viruses. American Journal of Tropical Medicine and Hygiene 33(1), 158-165.
4. IGARASHI, A (1978) Isolation of a Singh's Aedes albopictus cell clone sensitive to Dengue and Chikungunga viruses. Journal of General Virology 40,531-544.
5. KUBERSKI, T.T. & ROSEN, L., (1977). A simple technique for the detection of dengue antigen in mosquitoes by immunofluorescence. American Journal of Tropical Medicine and Hygiene 26, 533-537.
6. KUNO, G. (1982). Dengue virus replication in a poliploid mosquito cell culture grown in serum-free medium. Journal of Clinical Microbiology, 16 (5), 851-855.
7. KUNO, G. (1983). Cultivation of mosquito cell lines in serum-free medium and their effects on Dengue virus replication. In Vitro 19(9), 707-713.
8. KUNO, G., HlNK, W.F. & BRIGGS, J.D. (1971). Growth promoting serum proteins for Aedes albopictus cells cultured in vitro. Joumal of lnsect Physiology, 17, 1865-1879.
9. KUNO, G., MOORE, C.G. & SATHER, G.E. (1982). Factors affecting syncitial development in Aedes pseudoscutellaria cells by Dengue viruses. American Journal of Tropical Medicine and Hygiene 30( 4), 870-875.
10. KUNO, G., GUBLER, D.J., VELEZ, M. & OUVER, A (1985). Comparative sensitivity of three mosquito cell lines for isolation of Dengue viruses. Bulletin of the World Health Organization, 63 (2), 279-286.
11. PAUL, S.D., SINGH, K.R.P. & BHAT, U.K.M. (1969). A study on the cytopathic effect of arboviruses on cultures from Aedes albopictus cell line. Indian Journal of Medical Research, 57, 339-348.
12. RACE, M., WILLIAMS, M.C. & AGOSTINI, C.F. (1979). Dengue in the Caribbean: virus isolation in a mosquito (Aedes pseudoscutellaris) cell line. Transactions of the Royal Society of Tropical Medicine and Hygiene, 73, 18-22.
13. RANDOLPH, V. B. & STOLLAR, V. (1990). Low pH-induced cell fusion in flavivirus-infected Aedes albopictus cell cultures. Journal of General Virology, 71, 1845-1850.
14. RECHCIGL JR., M. (1977). CRC Handbook series in Nutrition and Food, section G: diets, food supplements, volume IV, culture media for cells, organs and embryos.
15. TESH, R.R (1979). A method for the isolation and identification of dengue viruses, using mosquito cell cultures. Journal of Tropical Medicine and Hygiene 28 (6), 1053 1059.
16. WATERMAN, S.H. & MONATH, T.P. (1982). Fluorescent antibody techniques applied to the identification of dengue virus in infected tissues. Acta Virologica, 26, 367-381.
17. WHITE, L.A., (1987). Susceptibility of Aedes albopictus C6/36 to viral infection. Journal of Clinical Microbiology, 25(7), 1221-1224.
2. CLARKE, D.H. & CASALS, J., (1958). Techniques for hemmaglutination and hemmaglutination-inhibition with arthropod-borne viruses. American Joumal of Tropical Medicine Hygiene 7, 561-573.
3. GUBLER, D.J., KUNO, G., SATHER, G.E., VELEZ, M. & OUVER, A (1984). Mosquito cell cultures and specific monoclonal antibodies in surveillance for Dengue viruses. American Journal of Tropical Medicine and Hygiene 33(1), 158-165.
4. IGARASHI, A (1978) Isolation of a Singh's Aedes albopictus cell clone sensitive to Dengue and Chikungunga viruses. Journal of General Virology 40,531-544.
5. KUBERSKI, T.T. & ROSEN, L., (1977). A simple technique for the detection of dengue antigen in mosquitoes by immunofluorescence. American Journal of Tropical Medicine and Hygiene 26, 533-537.
6. KUNO, G. (1982). Dengue virus replication in a poliploid mosquito cell culture grown in serum-free medium. Journal of Clinical Microbiology, 16 (5), 851-855.
7. KUNO, G. (1983). Cultivation of mosquito cell lines in serum-free medium and their effects on Dengue virus replication. In Vitro 19(9), 707-713.
8. KUNO, G., HlNK, W.F. & BRIGGS, J.D. (1971). Growth promoting serum proteins for Aedes albopictus cells cultured in vitro. Joumal of lnsect Physiology, 17, 1865-1879.
9. KUNO, G., MOORE, C.G. & SATHER, G.E. (1982). Factors affecting syncitial development in Aedes pseudoscutellaria cells by Dengue viruses. American Journal of Tropical Medicine and Hygiene 30( 4), 870-875.
10. KUNO, G., GUBLER, D.J., VELEZ, M. & OUVER, A (1985). Comparative sensitivity of three mosquito cell lines for isolation of Dengue viruses. Bulletin of the World Health Organization, 63 (2), 279-286.
11. PAUL, S.D., SINGH, K.R.P. & BHAT, U.K.M. (1969). A study on the cytopathic effect of arboviruses on cultures from Aedes albopictus cell line. Indian Journal of Medical Research, 57, 339-348.
12. RACE, M., WILLIAMS, M.C. & AGOSTINI, C.F. (1979). Dengue in the Caribbean: virus isolation in a mosquito (Aedes pseudoscutellaris) cell line. Transactions of the Royal Society of Tropical Medicine and Hygiene, 73, 18-22.
13. RANDOLPH, V. B. & STOLLAR, V. (1990). Low pH-induced cell fusion in flavivirus-infected Aedes albopictus cell cultures. Journal of General Virology, 71, 1845-1850.
14. RECHCIGL JR., M. (1977). CRC Handbook series in Nutrition and Food, section G: diets, food supplements, volume IV, culture media for cells, organs and embryos.
15. TESH, R.R (1979). A method for the isolation and identification of dengue viruses, using mosquito cell cultures. Journal of Tropical Medicine and Hygiene 28 (6), 1053 1059.
16. WATERMAN, S.H. & MONATH, T.P. (1982). Fluorescent antibody techniques applied to the identification of dengue virus in infected tissues. Acta Virologica, 26, 367-381.
17. WHITE, L.A., (1987). Susceptibility of Aedes albopictus C6/36 to viral infection. Journal of Clinical Microbiology, 25(7), 1221-1224.
Este trabalho está licenciado sob uma licença Creative Commons Attribution 4.0 International License.
Copyright (c) 1993 Maria Luisa, Barbosa, lray Maria Rocco, Julia M. M. Souza Felippe, Aurea Silveira Cruz
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