Resumen
Os vírus dengue são patógenos que vêm afetando milhões de pessoas durante os dois últimos séculos. No presente trabalho, comparou-se a técnica tradicional de MAC-ELISA e o teste de Immunoblotting, utilizando-se proteínas recombinantes, para pesquisa de anticorpos da classe M, contra os vírus Dengue sorotipos 1 e 2. Foram testadas 100 amostras de soro de pacientes, com suspeita clínica de dengue, por MAC-ELISA e Immunoblotting. Estes soros quando submetidos ao MAC-ELISA resultaram 56 positivos para dengue e 44 negativos. Estas mesmas amostras avaliadas por Immunoblotting revelaram 56 soros negativos, 36 soros positivos para DEN-l, 6 para DEN-2 e 2 para DEN-l e 2. Proteínas recombinantes (DEN-l e DEN-2) utilizadas como antígeno em Immunoblotting possibilitam um diagnóstico diferencial dos sorotipos de dengue circulantes em determinada área, enquanto o teste de MAC-ELISA apesar de sensível e rápido não tipifica o sorotipo de dengue, dado importante durante os períodos de epidemias.
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