Keywords
Dengue
MAC-ELISA
Irnmunoblotting
reeombinants proteins
MAC-ELISA
Irnmunoblotting
reeombinants proteins
How to Cite
1.
Luisa Barbosa M, Maria M. Souza-Felippe J, A. Fonseca B, N. Manson P, I. B. Ferreira I, Antonio Jerez J. Recombinant proteins for discriminating Dengue virus serotypes 1 and 2. Rev Inst Adolfo Lutz [Internet]. 1999 Dec. 30 [cited 2026 Jul. 26];58(2):121-7. Available from: https://periodicos.saude.sp.gov.br/RIAL/article/view/36704
Abstract
Dengue viruses (DEN-1 to 4) are human pathogens affeeting millions of people in the last two eenturies. Results of a eomparative study between the traditional MAC-ELISA method and the Immunoblotting teehniques using reeombinant proteins of DEN-l and DEN-2 to deteet virus speeifie class M Immunoglobulins, are reported in this paper. One hundred sera samples were studied. Fifty six samples were positive to dengue by MAC-ELISA and 44 were positive by Immunoblotting teehnique: 36 to DEN-1, 6 to DEN-2 and 2 to DEN-1 and 2 sorotypes. So, the serotype identifieation of the dengue virus eireulantig in a determined area is possible by using these reeombinant proteins in Immunoblotting teehniques. These data are important for epidemiologieal surveillanee mainly during the oeeurrenee of an epidemie
References
1. ALCARAZ, c., RODRIGUEZ, E; OVIEDO,J.M.; EIRAS, A; De DIEGO, M.; ALONSO, C. & ESCRIBANO, J.M .. Highly specific confirmatory Western blot test for African Swine Fever virus detection using the recombinant protein p54. J. Virol. Me thods , 53: 111-119, 1995.
2. AUSUBEL, EM.; BRENT, R; KINGSTON, RE.; MOORE, D.D.; SEIDMAN,J.G. & STRURHL, RE. Current protocols in molecular hiology. New York, John Wiley & Sons, 1989.
3. BURNETTE, W.N.. "Western blotting": eletropho-retic transfer of proteins from sodium dodecyl sulfate-polyacrylarnide gels to unrnodified nitrocellulose and radiographic detection with antibody and radiated protein A Anal. Biochem., 112: 195-203, 1981.
4. CHAMBERS, T.J., HAHN, C.S., GALLER, R & RICE, C.M., Flavivirus genome organization expression, and replication. Annu. Rev. Microhiol., 44: 649- 688, 1990.
5. CHURDBOONCHART, v, BHAMARAPRAVITI, N.; PEAMPRAMPRECHA, S. & SIRINAVIN, S.. Antibodies Against Dengue viral proteins in primary and Secondary Dengue Hemorrhagic Fever. Am. J. Trop. Med. Hyg., 44: 481-493,1991.
6. CHURDBOONCHART, v, BHAMARAPRAVITI, N.; PEAMPRAMPRECHA, S. PINTHONG, C.; SIRINAVIN, S. & CHAIYOTHA, A. Antibodies to dengue viral polypeptides. r. Sensitivity and specificity of the viral-antigen-stripsl enzyme immunoassay. South. Asian J. Trop. Med. Publie Health, 18: 362-372, 1987.
7. CLARKE, D.H. & CASALS, L Tecniques for hemagglutination and hemagglutination- inhibition with arthropod-borne viruses. Am. J. Trop. Med. Hyg., 7: 551, 1958.
8. FONSECA B.A.L., KHOSHNOOD K.,SHOPE RE. & MANSON P.W.. Flavivirus Type- Specific Antigens Produced from Fusions of a Portion of the E Protein Gene with the Escherichia coli TrpE Gene. Am. J. Trop. Med. Hyg., 44: 500-508, 1991.
9. GUBLER, D.L DenguelDengue Hemorrhagic fever in the Americas: Prospects for the year 2000. pg-19-27. ln: Dengue: A Wordwide Problem, a Common Strategy. Ed. by S.B. Halstead & Gomez- Dantes, 1992.
10. HALL, R.A.; KAY,H.B.; BURGESS, G.W.; CLANCY, P.& FINNING, 1.D.. Epitope analysis of the envelope and non-structural glycoproteins of Murray Valley encephalitis vírus. J. Gen. Virol., 71 :2923-2930, 1990.
11. HALSTEAD, S.B .. Pathogenesis of Dengue: Challenges to Molecular Biology- Seienee, 239: 476- 481, 1988
12. HASEGAWA, W.; YOSHIDA, M.; SHIOSAKA, T.; FUJITA, S. & KOBAYASHI, Y.. Mutations in the envelope protein of Japanese encephalitis virus affect entry into cultured cells and virulence in mice. Virology, 191: 158-165, 1992.
13. HEINZ, F.X .. Epitope mapping of flavivirus glycoproteins. Adv. Virus Res., 31: 103-168, 1986.
14. HENCHAL, E.A.; McCOWN, J.M.; BURKE, D.S.; SEGUIN, M.e. & BRANDT, W.E .. Epitopic analysis of antigenic determinants on the surface of dengue-2 virions using monoclonal antibodies. Am. J. Trop. Med. Hyg., 34: 162-167, 1985.
15. KUNO, G.; GÓMEZ, L & GUBLER, D.L Detecting artificial anti-dengue IgM Immune complexes using an enzyme-linked immunosorbent assay. Am Trop. Med. Hyg., 36: 153-159, 1987.
16. LINDO, J.F.; CONWAY,D.J.; ATKINS, N.S.; BIANCO,A.E.; ROBINSON, RD. & BUNDY, D.A.P .. Prospective evaluation of enzyme-linked immunosorvent assay and immunoblot methods for the diagnosis of endemic Trongyloides stercoralis infection. Am J. Trop. Med. Hyg., 51: 175-179, 1994.
17. MANDL, C.W.; HEINZ, F.X.; STOCKL, E. & KUNZ, c.. Genome sequence of tick-borne encephalitis virus ( Western subtype) and comparative analysis of nonstructural proteins with other Flaviviruses. Virology, 173: 291-301, 1989.
18. MASON, P.W.; DALRYMPLE, J.M.; GENTRY, M.K.; McCOWN, IM.; HOKE, c.n., BURKE, D.S; FOURNIER, M.J. & MASON, T.L.. Molecular Characterization of the Japanese Encephalitis Virus Structural Glycoprotein. J. Gen. Virol., 70: 2037- 2049, 1989.
19. MASON, P.W.; ZUGEL, M.U.; SEMPRONI, A.R; FOURNIER, M.J. & MASON, T.L.. The antigenic structure of dengue type 1 virus envelope and NS 1 proteins expressed in Escherichia coli. J. Gen. Virol., 71: 2107-2114, 1990.
20. PAN AMERICAN HEALTH ORGANIZATION (PAHO). Dengue and dengue hemorrhagic fever in the Americas: Guidelines for prevention and control. Washington, o.c. PAHO, 1994.
21. RICE, e.M.; STRAUSS, E.G. & STRAUSS, J.H. Structure of the flavivirus genome. ln: S. Schlesinger & M.J. Schlesinger. Togaviridae and Flaviviridae, New York: Plenum, 1986, 279326.
22. ROEHRIG, J.T.; JOHNSON, A.J.; HUNT, A.R; BOLIN, RA. & CHU, M.C.. Antibodies to dengue 2 virus E-glycoprotein synthetic peptides identify antigenic conformation. Virology, 177: 668-675, 1990.
23. ROEHRIG, J.T.; MATHEWS, J.H. & TRENT, D.W .. ldentification of epitopes on the E glycoprotein of St. Louis encephalitis virus using monoclonal antibodies. Virology, 128: 118, 1983.
24. SAMBROOK, I; FRITSCH, E.F. & MANIA TIS , T. Moleeular cloning: a laboratory manual. 2nd. ed., New York, Cold Spring Harbor Laboratory Press, 1989.
25. SELB, B. & WEBER, B.. A study 01'human reovirus IgG and IgA antibodies by ELISA and Western blot. J. ViroI. Methods, 47: 15-26, 1994.
26. SMITH, G.W. & WHIGHT, P.L Synthesis 01'proteins and glycoproteins in dengue type 2 virusinfected Vero and Aedes albopictus cells. 1. Gen. Virol., 66: 559-571, 1985.
27. TOWBIN, H.; STAEHELIN, T. & GORDON, L Eletrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proe. Natl. Aead. Sei. USA, 76: 4350-4354, 1979.
28. VOLLER, A. &BIDWELL, D.. Enzime-linked immunosorbent assay. ln Rose ,N.R.I Fridman, H & Fahey, IH. eds. Manual of Clinical Laboratory Immunology. Washington, De American Society for Microbiology. 99-109, 1986
29. WESTAWAY, E.O. Flavivirus rep1ication strategy. Adv. Vírus Res., 33: 45-90 1987.
30. WESTAWAY, E.O. Rep1ication of Flavivirus ln: The Togaviruses: Biology, Structure, Replication. New York: Academic Press, 1980,531- 581.
2. AUSUBEL, EM.; BRENT, R; KINGSTON, RE.; MOORE, D.D.; SEIDMAN,J.G. & STRURHL, RE. Current protocols in molecular hiology. New York, John Wiley & Sons, 1989.
3. BURNETTE, W.N.. "Western blotting": eletropho-retic transfer of proteins from sodium dodecyl sulfate-polyacrylarnide gels to unrnodified nitrocellulose and radiographic detection with antibody and radiated protein A Anal. Biochem., 112: 195-203, 1981.
4. CHAMBERS, T.J., HAHN, C.S., GALLER, R & RICE, C.M., Flavivirus genome organization expression, and replication. Annu. Rev. Microhiol., 44: 649- 688, 1990.
5. CHURDBOONCHART, v, BHAMARAPRAVITI, N.; PEAMPRAMPRECHA, S. & SIRINAVIN, S.. Antibodies Against Dengue viral proteins in primary and Secondary Dengue Hemorrhagic Fever. Am. J. Trop. Med. Hyg., 44: 481-493,1991.
6. CHURDBOONCHART, v, BHAMARAPRAVITI, N.; PEAMPRAMPRECHA, S. PINTHONG, C.; SIRINAVIN, S. & CHAIYOTHA, A. Antibodies to dengue viral polypeptides. r. Sensitivity and specificity of the viral-antigen-stripsl enzyme immunoassay. South. Asian J. Trop. Med. Publie Health, 18: 362-372, 1987.
7. CLARKE, D.H. & CASALS, L Tecniques for hemagglutination and hemagglutination- inhibition with arthropod-borne viruses. Am. J. Trop. Med. Hyg., 7: 551, 1958.
8. FONSECA B.A.L., KHOSHNOOD K.,SHOPE RE. & MANSON P.W.. Flavivirus Type- Specific Antigens Produced from Fusions of a Portion of the E Protein Gene with the Escherichia coli TrpE Gene. Am. J. Trop. Med. Hyg., 44: 500-508, 1991.
9. GUBLER, D.L DenguelDengue Hemorrhagic fever in the Americas: Prospects for the year 2000. pg-19-27. ln: Dengue: A Wordwide Problem, a Common Strategy. Ed. by S.B. Halstead & Gomez- Dantes, 1992.
10. HALL, R.A.; KAY,H.B.; BURGESS, G.W.; CLANCY, P.& FINNING, 1.D.. Epitope analysis of the envelope and non-structural glycoproteins of Murray Valley encephalitis vírus. J. Gen. Virol., 71 :2923-2930, 1990.
11. HALSTEAD, S.B .. Pathogenesis of Dengue: Challenges to Molecular Biology- Seienee, 239: 476- 481, 1988
12. HASEGAWA, W.; YOSHIDA, M.; SHIOSAKA, T.; FUJITA, S. & KOBAYASHI, Y.. Mutations in the envelope protein of Japanese encephalitis virus affect entry into cultured cells and virulence in mice. Virology, 191: 158-165, 1992.
13. HEINZ, F.X .. Epitope mapping of flavivirus glycoproteins. Adv. Virus Res., 31: 103-168, 1986.
14. HENCHAL, E.A.; McCOWN, J.M.; BURKE, D.S.; SEGUIN, M.e. & BRANDT, W.E .. Epitopic analysis of antigenic determinants on the surface of dengue-2 virions using monoclonal antibodies. Am. J. Trop. Med. Hyg., 34: 162-167, 1985.
15. KUNO, G.; GÓMEZ, L & GUBLER, D.L Detecting artificial anti-dengue IgM Immune complexes using an enzyme-linked immunosorbent assay. Am Trop. Med. Hyg., 36: 153-159, 1987.
16. LINDO, J.F.; CONWAY,D.J.; ATKINS, N.S.; BIANCO,A.E.; ROBINSON, RD. & BUNDY, D.A.P .. Prospective evaluation of enzyme-linked immunosorvent assay and immunoblot methods for the diagnosis of endemic Trongyloides stercoralis infection. Am J. Trop. Med. Hyg., 51: 175-179, 1994.
17. MANDL, C.W.; HEINZ, F.X.; STOCKL, E. & KUNZ, c.. Genome sequence of tick-borne encephalitis virus ( Western subtype) and comparative analysis of nonstructural proteins with other Flaviviruses. Virology, 173: 291-301, 1989.
18. MASON, P.W.; DALRYMPLE, J.M.; GENTRY, M.K.; McCOWN, IM.; HOKE, c.n., BURKE, D.S; FOURNIER, M.J. & MASON, T.L.. Molecular Characterization of the Japanese Encephalitis Virus Structural Glycoprotein. J. Gen. Virol., 70: 2037- 2049, 1989.
19. MASON, P.W.; ZUGEL, M.U.; SEMPRONI, A.R; FOURNIER, M.J. & MASON, T.L.. The antigenic structure of dengue type 1 virus envelope and NS 1 proteins expressed in Escherichia coli. J. Gen. Virol., 71: 2107-2114, 1990.
20. PAN AMERICAN HEALTH ORGANIZATION (PAHO). Dengue and dengue hemorrhagic fever in the Americas: Guidelines for prevention and control. Washington, o.c. PAHO, 1994.
21. RICE, e.M.; STRAUSS, E.G. & STRAUSS, J.H. Structure of the flavivirus genome. ln: S. Schlesinger & M.J. Schlesinger. Togaviridae and Flaviviridae, New York: Plenum, 1986, 279326.
22. ROEHRIG, J.T.; JOHNSON, A.J.; HUNT, A.R; BOLIN, RA. & CHU, M.C.. Antibodies to dengue 2 virus E-glycoprotein synthetic peptides identify antigenic conformation. Virology, 177: 668-675, 1990.
23. ROEHRIG, J.T.; MATHEWS, J.H. & TRENT, D.W .. ldentification of epitopes on the E glycoprotein of St. Louis encephalitis virus using monoclonal antibodies. Virology, 128: 118, 1983.
24. SAMBROOK, I; FRITSCH, E.F. & MANIA TIS , T. Moleeular cloning: a laboratory manual. 2nd. ed., New York, Cold Spring Harbor Laboratory Press, 1989.
25. SELB, B. & WEBER, B.. A study 01'human reovirus IgG and IgA antibodies by ELISA and Western blot. J. ViroI. Methods, 47: 15-26, 1994.
26. SMITH, G.W. & WHIGHT, P.L Synthesis 01'proteins and glycoproteins in dengue type 2 virusinfected Vero and Aedes albopictus cells. 1. Gen. Virol., 66: 559-571, 1985.
27. TOWBIN, H.; STAEHELIN, T. & GORDON, L Eletrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proe. Natl. Aead. Sei. USA, 76: 4350-4354, 1979.
28. VOLLER, A. &BIDWELL, D.. Enzime-linked immunosorbent assay. ln Rose ,N.R.I Fridman, H & Fahey, IH. eds. Manual of Clinical Laboratory Immunology. Washington, De American Society for Microbiology. 99-109, 1986
29. WESTAWAY, E.O. Flavivirus rep1ication strategy. Adv. Vírus Res., 33: 45-90 1987.
30. WESTAWAY, E.O. Rep1ication of Flavivirus ln: The Togaviruses: Biology, Structure, Replication. New York: Academic Press, 1980,531- 581.
This work is licensed under a Creative Commons Attribution 4.0 International License.
Copyright (c) 1999 Instituto Adolfo Lutz Journal
Downloads
Download data is not yet available.