Inoculação de Mycobacterium leprae na bolsa júgal do hamster
DOI:
https://doi.org/10.47878/hi.1999.v24.35403Palavras-chave:
Hamster, Bolsa Jugal, Granuloma, Mycobacterium lepraeResumo
A utilização da bolsa jugal do hamster, foi avaliada pela inoculação de 6,0x108 M. leprae/ml no tecido subepitelial de 60 animais, empregando como grupo controle, 12 hamsters inoculados no coxim plantar. Os animais foram sacrificados em 20 e 48 horas e aos 7,14,21 e 28 dias pi. A evolução da lesão de inoculação foi analisada pelo exame histológico em cortes corados pela hema-toxílina-eosina e Faraco-Fite. A avaliação da viabilidade bacilar na bolsa fugal do hamster foi realizada 7, 14, 21, e 28 dias pi pelo teste de recuperação bacilar em camundongos. Os resultados nos permitiram concluir que a resposta inflamatória ao M. leprae na bolsa jugal evoluiu para formação de granulomas macro-fágicos semelhantes aos da hanseníase virchoviana humana porém, o teste de recuperação bacilar sugeriu que não houve multiplicação dos bacilos. As lesões do coxim plantar evoluíram para granulomas epitelióides semelhantes aos da hanseníase dimorfa.
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Referências
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5. BASKIN, G.B., BOBBY,J.G., MARTIN,L.N., WOLF, R.H., MURPHEY-CORB, M., WALSH, G.P., BINFORD, C.H., MEYERS, W.M., MALATY, R.
Experimental leprosy in rhesus monkey: necropsy findings. Int. J. Leprosy, v.55, p. 109-15, 1987.
6. BERGSTRESSER, P.R., FLETCHER, C.R., STREILEIN, J.W. Surface densities of Largerhans cells in relation to rodent epidermal sites with special immunologic properties. J. Invest. Dermatol., v.74, p.77-80, 1980.
7. BINFORD, C.H., WALSH, G.P., THEOCHUNG, T.L. Transmission of Mycobacterium leprae in immunosuppressed mice. Use of bone
marrow shielding in preventing death from irradiation. Int. J. Leprosy, v.40, p.99-100, 1972.
8. CHEHL, S., JOB, C.K., HASTINGS, R.C. Transmission of leprosy in nude mice. Am. J. Trop. Med.Hyg., v.34, p.1161, 1985
9. COLSTON, M.J., HILSON, G.R.F. Growth of Mycobacterium leprae and M. marinum in congenitally athymic (nude) mice. Nature,
v.262, p.399-401, 1976.
10. CONVIT, J., LAPENTA, P., ILUKEVICH, A., IMAEDA, T. Experimental inoculation of human leprosy in laboratory animals. I. Clinical, bacteriologic, and histopathologic study. Int. J. Leposy, v.30, p.239-53, 1962.
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Higiene e Medicina Tropical, 1994. p.50-6.
12. FARACO, J. Bacillos de Hansen e cortes de parafina; methodo complementar para pesquisa de bacillos de Hansen em cortes de
material incluído em parafina. Rev. bras. Leprol., v.6, p.177-80, 1938.
13. FIELDSTEEL, A.H., MC INTOSH, A.M. Effect of neonatal thymectomy and antithymocytic serum on s us cep t ib ilit y of ra ts t o
Mycobacterium leprae. Proc. Soc. Exp. Biol. Med., v. 138, p. 8-13, 1971.
14. FIELDSTEEL, A.H., SATO, N., COLSTON, M.J. Relationship between T-cell population in neonatally thymectomized Lewis rats and
susceptibility to infection with Mycobacterium leprae. Int. J. Leprosy, v. 49, p.317-23, 1981.
15. FITE, G.L. Staining of acid-fast bacilli in paraffin sections. Am. J. Pathol., v. 14, p.491-507, 1938.
16. GAUGAS, J.M., REES, R.J.W., WEDDELL, A.G.M. Reversal effect of thymus grafts on lepromatous leprosy in thymectomised- irradiated mice. Int. J. Leprosy, v.39, p. 388-95, 1971.
17. GORMUS, B.J., XU, K., BASKIN, G.B., MARTIN, L.N., BOHM, R.P., BLANCHARD, J.L., MACK, PA, RATTERREE, M.S., McCLURE, H.M.,
MEYERS, W.M., WALSH, G.P. Experimental leprosy in monkeys. I. sooty mangabey monkey: transmission, susceptibility, clinical and pathological findings. Leprosy Review. v.66, p.96-104, 1995.
18. HANDLER, A.H., SHEPRO, D. Cheek pouch technology: Uses and applications. In: H O F F M A N , R . A , R O B I N S O N , F . P ,
MAGALHÃES, H. The golden hamsters. Its biology and uses in medical research. Iowa City: Iowa State University Press, 1968. p.104-201.
19. HANSEN, G.H.A. Üdersogelser angaend spedalskhendens arsager. Norsk. Laegevil., v.4, p.1-88, 1873.
20. HARDY, M.H., VRABLIC, O.E., COVANT, H.A., KANDARKAR, S.V. The development of the Syrian hamster cheek pouch. Anat. Rec.,
v.214, p.273-82,1986
21. KIRCHHEIMER, W.F., STORRS, E.E. Attemps to stablish the armadillo (Dasypus novemcinctus) as a model for the study of leprosy. I. report of lepromatous leprosy in experimentally infect armadillo. Int. J. Leprosy, v. 39, p.693-702, 1971.
22. REES, R.J.W. Enhanced susceptibility of thymectomized and irradiated to infection with Mycobacterium leprae. Nature, v.211, p.657-8, 1966
23. REES, R.J.W., WEDDELL, A.G.M., PALMER, E., PEARSON, J.M.H. Human leprosy in normal mice. Br. Med. J., v.3, p.216-7, 1969.
24. RIDLEY, D.S., HILSON, G.R.F. A logarithmic index of bacilli in biopsies. I. Method . Int. J. Leprosy, v.35, p.184-6, 1967.
25. ROXO, E., SINHORINI, I.L., MARIANO, M. Evolução das lesões induzidas por micobactérias BCG e por H37 RV em hamster (Mesocricetus auratus). Hans. Int., v.19, p.19-25, 1994.
26. SAMPAIO, M.M., DIAS, L.B. Experimental infection of Jorge Lobo's disease in the cheek pouch of the golden hamster. Rev. Inst. Med. Trop. São Paulo, v.12, p.115-20, 1970.
27. SHEPARD, C.C. The experimental disease that follows the injection of human leprosy bacilli into the foot pads of mice. J. Exp. Med., v. 112, p. 445-54, 1960.
28. SHEPARD, C.C., VAN-LANDINGHAM, R.M., WALKER, L.L., YES, Z. Comparison of the immunogenecity vaccines prepared from
viable M .bovis, heat-killed M. leprae, and a mixture of the two for normal and M. lepraetolerant mice. Iníect Immun., v. 40, p.1096,
1983.
29. SINHORINI, I.L., MERUSSE, J.L.B., MARIANO, M. Role of lymphatic drainage on the development of Calmette-Guerin Bacillus -
induced granulomas in the hamster. int. Arch. Allergy Immunol., v.103, p.166-74, 1994.
30. SU, D.W.P., YANG, H.Y., SKINSNES, O.K. The e f f e c t o f n e o n a t a l t h y m e c t o m y o n Mycobacterium leprae infection in mice. Int. J. Leprosy, v.41, p.81-92, 1973.
31. WHITE, F.H., GOHARI, K. The ultrastrutural m or p ho log y o f h a m s t e r c he e k p o uc h epithelium. Arch. Oral Biol., v.26, p. 563-69, 1981.
32. WOLF, R.H., GORMUS, B.J., MARTIN, L.N. Experimental leprosy in the three species of monkeys. Science. v. 227, p. 529-31, 1985.
33. WORLD HEALTH ORGANIZATION Laboratory Techniques for Leprosy. Geneva: WHO\CDS\LEP\ 86.4: 1987. p.134.
34. YOGI, Y., NAKAMURA, K., IONOUYE, T. Susceptibility o f severe C ombined immunodeficient ( S C I D ) mice to Mycobacterium leprae: multiplication of M. leprae inoculated into both hind feet at an early stage. Int. J. Leprosy, v. 59, p. 722-3, 1991.
2. ARRUDA, M.S.P., FLEURY, R.N., NOGUEIRA, M.E.S. Inoculation of Mycobacterium leprae into the cheek pouch. Leprosy Review, v.66,
p.181-2, 1995a.
3. ARRUDA, M.S.P., MOI, A.L.M., CLARO, S.G., NOGUEIRA, M.E.S. Drenagem linfática no tecido conjuntivo da bolsa jugal do hamster.
Salusvita, v.14, p109-13, 19956.
4. BARKER, E., BILLIGHAM, R.E. Immunologicaly privileged sites. Adv. Immunol., v.25, p.1-54, 1977,
5. BASKIN, G.B., BOBBY,J.G., MARTIN,L.N., WOLF, R.H., MURPHEY-CORB, M., WALSH, G.P., BINFORD, C.H., MEYERS, W.M., MALATY, R.
Experimental leprosy in rhesus monkey: necropsy findings. Int. J. Leprosy, v.55, p. 109-15, 1987.
6. BERGSTRESSER, P.R., FLETCHER, C.R., STREILEIN, J.W. Surface densities of Largerhans cells in relation to rodent epidermal sites with special immunologic properties. J. Invest. Dermatol., v.74, p.77-80, 1980.
7. BINFORD, C.H., WALSH, G.P., THEOCHUNG, T.L. Transmission of Mycobacterium leprae in immunosuppressed mice. Use of bone
marrow shielding in preventing death from irradiation. Int. J. Leprosy, v.40, p.99-100, 1972.
8. CHEHL, S., JOB, C.K., HASTINGS, R.C. Transmission of leprosy in nude mice. Am. J. Trop. Med.Hyg., v.34, p.1161, 1985
9. COLSTON, M.J., HILSON, G.R.F. Growth of Mycobacterium leprae and M. marinum in congenitally athymic (nude) mice. Nature,
v.262, p.399-401, 1976.
10. CONVIT, J., LAPENTA, P., ILUKEVICH, A., IMAEDA, T. Experimental inoculation of human leprosy in laboratory animals. I. Clinical, bacteriologic, and histopathologic study. Int. J. Leposy, v.30, p.239-53, 1962.
11. DAVID, H., BRUM, L., PRIETO, E. Manual de micobacteriologia em saúde pública: Princípios e Métodos. Lisboa: Instituto de
Higiene e Medicina Tropical, 1994. p.50-6.
12. FARACO, J. Bacillos de Hansen e cortes de parafina; methodo complementar para pesquisa de bacillos de Hansen em cortes de
material incluído em parafina. Rev. bras. Leprol., v.6, p.177-80, 1938.
13. FIELDSTEEL, A.H., MC INTOSH, A.M. Effect of neonatal thymectomy and antithymocytic serum on s us cep t ib ilit y of ra ts t o
Mycobacterium leprae. Proc. Soc. Exp. Biol. Med., v. 138, p. 8-13, 1971.
14. FIELDSTEEL, A.H., SATO, N., COLSTON, M.J. Relationship between T-cell population in neonatally thymectomized Lewis rats and
susceptibility to infection with Mycobacterium leprae. Int. J. Leprosy, v. 49, p.317-23, 1981.
15. FITE, G.L. Staining of acid-fast bacilli in paraffin sections. Am. J. Pathol., v. 14, p.491-507, 1938.
16. GAUGAS, J.M., REES, R.J.W., WEDDELL, A.G.M. Reversal effect of thymus grafts on lepromatous leprosy in thymectomised- irradiated mice. Int. J. Leprosy, v.39, p. 388-95, 1971.
17. GORMUS, B.J., XU, K., BASKIN, G.B., MARTIN, L.N., BOHM, R.P., BLANCHARD, J.L., MACK, PA, RATTERREE, M.S., McCLURE, H.M.,
MEYERS, W.M., WALSH, G.P. Experimental leprosy in monkeys. I. sooty mangabey monkey: transmission, susceptibility, clinical and pathological findings. Leprosy Review. v.66, p.96-104, 1995.
18. HANDLER, A.H., SHEPRO, D. Cheek pouch technology: Uses and applications. In: H O F F M A N , R . A , R O B I N S O N , F . P ,
MAGALHÃES, H. The golden hamsters. Its biology and uses in medical research. Iowa City: Iowa State University Press, 1968. p.104-201.
19. HANSEN, G.H.A. Üdersogelser angaend spedalskhendens arsager. Norsk. Laegevil., v.4, p.1-88, 1873.
20. HARDY, M.H., VRABLIC, O.E., COVANT, H.A., KANDARKAR, S.V. The development of the Syrian hamster cheek pouch. Anat. Rec.,
v.214, p.273-82,1986
21. KIRCHHEIMER, W.F., STORRS, E.E. Attemps to stablish the armadillo (Dasypus novemcinctus) as a model for the study of leprosy. I. report of lepromatous leprosy in experimentally infect armadillo. Int. J. Leprosy, v. 39, p.693-702, 1971.
22. REES, R.J.W. Enhanced susceptibility of thymectomized and irradiated to infection with Mycobacterium leprae. Nature, v.211, p.657-8, 1966
23. REES, R.J.W., WEDDELL, A.G.M., PALMER, E., PEARSON, J.M.H. Human leprosy in normal mice. Br. Med. J., v.3, p.216-7, 1969.
24. RIDLEY, D.S., HILSON, G.R.F. A logarithmic index of bacilli in biopsies. I. Method . Int. J. Leprosy, v.35, p.184-6, 1967.
25. ROXO, E., SINHORINI, I.L., MARIANO, M. Evolução das lesões induzidas por micobactérias BCG e por H37 RV em hamster (Mesocricetus auratus). Hans. Int., v.19, p.19-25, 1994.
26. SAMPAIO, M.M., DIAS, L.B. Experimental infection of Jorge Lobo's disease in the cheek pouch of the golden hamster. Rev. Inst. Med. Trop. São Paulo, v.12, p.115-20, 1970.
27. SHEPARD, C.C. The experimental disease that follows the injection of human leprosy bacilli into the foot pads of mice. J. Exp. Med., v. 112, p. 445-54, 1960.
28. SHEPARD, C.C., VAN-LANDINGHAM, R.M., WALKER, L.L., YES, Z. Comparison of the immunogenecity vaccines prepared from
viable M .bovis, heat-killed M. leprae, and a mixture of the two for normal and M. lepraetolerant mice. Iníect Immun., v. 40, p.1096,
1983.
29. SINHORINI, I.L., MERUSSE, J.L.B., MARIANO, M. Role of lymphatic drainage on the development of Calmette-Guerin Bacillus -
induced granulomas in the hamster. int. Arch. Allergy Immunol., v.103, p.166-74, 1994.
30. SU, D.W.P., YANG, H.Y., SKINSNES, O.K. The e f f e c t o f n e o n a t a l t h y m e c t o m y o n Mycobacterium leprae infection in mice. Int. J. Leprosy, v.41, p.81-92, 1973.
31. WHITE, F.H., GOHARI, K. The ultrastrutural m or p ho log y o f h a m s t e r c he e k p o uc h epithelium. Arch. Oral Biol., v.26, p. 563-69, 1981.
32. WOLF, R.H., GORMUS, B.J., MARTIN, L.N. Experimental leprosy in the three species of monkeys. Science. v. 227, p. 529-31, 1985.
33. WORLD HEALTH ORGANIZATION Laboratory Techniques for Leprosy. Geneva: WHO\CDS\LEP\ 86.4: 1987. p.134.
34. YOGI, Y., NAKAMURA, K., IONOUYE, T. Susceptibility o f severe C ombined immunodeficient ( S C I D ) mice to Mycobacterium leprae: multiplication of M. leprae inoculated into both hind feet at an early stage. Int. J. Leprosy, v. 59, p. 722-3, 1991.
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30-06-1999
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Nogueira MES, Coelho K labuki R, Fleury RN, Arrudas MSP de. Inoculação de Mycobacterium leprae na bolsa júgal do hamster. Hansen. Int. [Internet]. 30º de junho de 1999 [citado 25º de abril de 2024];24(1):5-14. Disponível em: https://periodicos.saude.sp.gov.br/hansenologia/article/view/35403
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