In vitro effect of Mycobacterium Leprae suspensions on the polymorphonuclear neutrophiles function of hanseníasis patients to candida albicans and Candida pseudotropicalis
DOI:
https://doi.org/10.47878/hi.1984.v9.35568Palavras-chave:
Mycobacterium leprae, Neutrophiles, Hanseniasis, Candida albicans, Candida pseudotropicalisResumo
The in vitro effect of Mycobacterium Ieprae suspensions on the PMN hability to phagocyting and killing Candida albicans and Candida pseudotropicalls was studied in forty-five patients of Hansen's disease and in fifteen healthy controls. Our results show no significative differences between the different studied groups, both for the phagocytosis and for the lysis of yeasts. There was no significant changes in the mean values of these functions after previous or simultaneously incubation with Mycobacterium Ieprae suspensions. Those observations confirmed that there are not alterations in the enzimatic battery of PMN in Hansen's disease patients and that the Mycobacterium Ieprae presence does not exert stimulating effect on this in vitro model.
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Referências
1. BARBIERI, TA. & CORREA, W.M. Human macrophage culture: the leprosy test (LPT). Int. J. Lepr., 35 (3): 377-378,1967.
2. BEIGUELMAN, B. Fate of Mycobacterium leprae in macrophages. Int. J. Lepr., 39 (4): 896, 1971.
3. BEIGUELMAN,B.Genéticanahanseníase. Hansen. Int., 3 (2): 179-193, 1978.
4. BOYDEM, S. The chemotactic effect of mixtures of antibody and antigen on polymorphonuclear leukocytes. J. Exp. Med, 115: 453-066,1962.
5. DRUTZ, DJ. & CLINE, MJ. Polymorphonuclear leukocyte and macrophage function in leprosy. Int. J. Lepr., 38 (3):352-353,1970.
6. ESTEVEZ, M.E. & SEN, L. Capacidad funclonal de los monocitos humanos normales: una simple técnica para su exploración. Sangre, 23: 870-875,1978.
7. ESTEVEZ, M.E.; SEN, L.; VADEZ, R.; BALINÃ, L.M. Defective blood mononuclear phagacite function in patients with leprosy. Int. J. Lepr., 47 (4): 575-579, 1979.
8 MESS, EL. Posible origen de la inmunodeficiencia en la hanseniasis virchowiana. Hansen. Int., 3 (2): 141-150, 1978.
9. FLIESS, E.L. & BACHMANN, A.E. Alteradón de la inmunidad mediada por células en enfermos de lepra. Rev. Asoc. Arg. Microbiol, 9 (1): 28-36, 1977.
10. FLIESS, EL.; COMESANÃ, G.; CARRA-RA, R.C.;ESPAÑA, A. Fagocitosis y lists de Candida albicans y Candida pseudotropicalis por polimorfonucleares neurtrófllos de pacientes hansenianos. Hansen. Int., 6 (2): 109-113, 1981.
11. GIUNTOLI, J.; ESTEVEZ, M.E.; SEN, L.; PEÑALVER, J. Deficiencia funcional de los polimorfonucleares en los enfermos con Talasemia mayor. Medici-na. 38: 835-836, 1978.
12. GOIHMAN-YAHR, M.; CONVIT, J.; RODRIGUEZ, OCHOA, G. N.B.T. test in lepromatous leprosy. Lancet, 2 (7826): 456-457,1973.
13. GOIHMAN-YAHR, M.; RODRIGUEZ OCHOA, G.; ARANZAZU, N.; PINARDI, M.E. ; GOMEZ,M.E.; OCANTO, A.; CONVIT, J. In vitro activation of neutrophils by suspencions of Mycobacterium leprae. Int. Lepr., 47 (4): 570-574, 1979.
14. GOLDE, D.W.; TERRITO, M.; FINLEY, TN.; CLINE, M.J. Defective lung macrophages in pulmonary alveolar proteinosis. Ann. Intern. Med., 85: 304-309, 1976.
15. KLEBANOFF, SJ. & HAMON, C.B. Role of myeloperoxidase mediated an timicrobial systems in intact leukocytes. J. Reticuloendothel. Soc., 12: 170-174,1972.
16. LACE, J.K.; TAN, J.S.; WATANABUNA KORN, C. An appraisal of the nitro- blue tetrazolium reduction test. Am. J. Med., 58: 685-694. 1975.
17. LEHRER, R.I. The fungicidal mechanisms of human monocytes. I. Evidence for myeloperoxidase-linked and myeloperoxidase-independent candidacidal mechanisms. J. Clip. Invest., 55: 338-346,1975.
18 EHRER, R.I. Inhibition by sulfonamides of the candidacidal activity of human neutrophils. J. Clin. Invest., 50: 2498-2505, 1971.
19. METCHNICOFF, E. Immunity in infective diseases. London, Cambridge University Press, 190.5.
20. MIRANDA, R.N. Confirmation in vitro of Mycobacterium leprae phagocytosis by polimorphonucleares. Leprologia, 19 (2): 206-209, 1974.
21. STENDAHL,O.;MOLIN,L.;DAHLGREN,C. The inhibition of polymorphonu clear leukocyte cytotoxicity by dap-sone. J. Clin. Invest., 62: 214-220, 1978.
2. BEIGUELMAN, B. Fate of Mycobacterium leprae in macrophages. Int. J. Lepr., 39 (4): 896, 1971.
3. BEIGUELMAN,B.Genéticanahanseníase. Hansen. Int., 3 (2): 179-193, 1978.
4. BOYDEM, S. The chemotactic effect of mixtures of antibody and antigen on polymorphonuclear leukocytes. J. Exp. Med, 115: 453-066,1962.
5. DRUTZ, DJ. & CLINE, MJ. Polymorphonuclear leukocyte and macrophage function in leprosy. Int. J. Lepr., 38 (3):352-353,1970.
6. ESTEVEZ, M.E. & SEN, L. Capacidad funclonal de los monocitos humanos normales: una simple técnica para su exploración. Sangre, 23: 870-875,1978.
7. ESTEVEZ, M.E.; SEN, L.; VADEZ, R.; BALINÃ, L.M. Defective blood mononuclear phagacite function in patients with leprosy. Int. J. Lepr., 47 (4): 575-579, 1979.
8 MESS, EL. Posible origen de la inmunodeficiencia en la hanseniasis virchowiana. Hansen. Int., 3 (2): 141-150, 1978.
9. FLIESS, E.L. & BACHMANN, A.E. Alteradón de la inmunidad mediada por células en enfermos de lepra. Rev. Asoc. Arg. Microbiol, 9 (1): 28-36, 1977.
10. FLIESS, EL.; COMESANÃ, G.; CARRA-RA, R.C.;ESPAÑA, A. Fagocitosis y lists de Candida albicans y Candida pseudotropicalis por polimorfonucleares neurtrófllos de pacientes hansenianos. Hansen. Int., 6 (2): 109-113, 1981.
11. GIUNTOLI, J.; ESTEVEZ, M.E.; SEN, L.; PEÑALVER, J. Deficiencia funcional de los polimorfonucleares en los enfermos con Talasemia mayor. Medici-na. 38: 835-836, 1978.
12. GOIHMAN-YAHR, M.; CONVIT, J.; RODRIGUEZ, OCHOA, G. N.B.T. test in lepromatous leprosy. Lancet, 2 (7826): 456-457,1973.
13. GOIHMAN-YAHR, M.; RODRIGUEZ OCHOA, G.; ARANZAZU, N.; PINARDI, M.E. ; GOMEZ,M.E.; OCANTO, A.; CONVIT, J. In vitro activation of neutrophils by suspencions of Mycobacterium leprae. Int. Lepr., 47 (4): 570-574, 1979.
14. GOLDE, D.W.; TERRITO, M.; FINLEY, TN.; CLINE, M.J. Defective lung macrophages in pulmonary alveolar proteinosis. Ann. Intern. Med., 85: 304-309, 1976.
15. KLEBANOFF, SJ. & HAMON, C.B. Role of myeloperoxidase mediated an timicrobial systems in intact leukocytes. J. Reticuloendothel. Soc., 12: 170-174,1972.
16. LACE, J.K.; TAN, J.S.; WATANABUNA KORN, C. An appraisal of the nitro- blue tetrazolium reduction test. Am. J. Med., 58: 685-694. 1975.
17. LEHRER, R.I. The fungicidal mechanisms of human monocytes. I. Evidence for myeloperoxidase-linked and myeloperoxidase-independent candidacidal mechanisms. J. Clip. Invest., 55: 338-346,1975.
18 EHRER, R.I. Inhibition by sulfonamides of the candidacidal activity of human neutrophils. J. Clin. Invest., 50: 2498-2505, 1971.
19. METCHNICOFF, E. Immunity in infective diseases. London, Cambridge University Press, 190.5.
20. MIRANDA, R.N. Confirmation in vitro of Mycobacterium leprae phagocytosis by polimorphonucleares. Leprologia, 19 (2): 206-209, 1974.
21. STENDAHL,O.;MOLIN,L.;DAHLGREN,C. The inhibition of polymorphonu clear leukocyte cytotoxicity by dap-sone. J. Clin. Invest., 62: 214-220, 1978.
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30-11-1984
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FLIESS EL, FRANCESCHINI G, ORTIZ MC. In vitro effect of Mycobacterium Leprae suspensions on the polymorphonuclear neutrophiles function of hanseníasis patients to candida albicans and Candida pseudotropicalis. Hansen. Int. [Internet]. 30º de novembro de 1984 [citado 4º de maio de 2024];9(1/2):3-9. Disponível em: https://periodicos.saude.sp.gov.br/hansenologia/article/view/35568
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