Yellow Fever diagnosis in formalin fixed and paraffin embedded non-human primates’ tissues: the importance of the histopathological examination
Keywords:
Arboviroses, Diagnóstico, Flavivirus, Histologia, ZoonosesAbstract
Epizootics of Yellow Fever in non-human primates (NHP) are indicative of viral
circulation of the Yellow Fever virus (YFV) and may predict the occurrence of human
cases. Therefore, adequate diagnosis of the disease in the different genera of NHP
occurring in Brazil is important for YF control strategies, such as vaccination. The
Pathology Center of the Adolfo Lutz Institute (CPA-IAL), a macro-regional reference
laboratory, participates in the Epizootic Surveillance Program through histopathological
and immunohistochemical exams for YF. This study presents the caseload received and
analyzed at the CPA-IAL during 2017, with especial emphasis on YF immunopositivity
in the liver. Samples from 2,171 NHPs were evaluated in 2017. From these, 626 (28.83%)
were positive by immunohistochemistry; 580 were well preserved and 132 were
autolyzed. Of those well preserved, 577 (99.48%) exhibited typical YF histopathological
changes in the liver, including massive necrosis/apoptosis with Councilman-Rocha Lima
bodies, steatosis and mild inflammatory infiltrate. Histopathology and IHQ agreement
was very good (kappa = 0,98). Anatomopathological tools are pivotal for the diagnosis
and monitoring of YF in NHP and may prove of great value even in carcasses with
advanced autolysis. The YF-associated histopathological findings in liver of NHP are
conclusive and should precede immunohistochemical screening method.
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Copyright (c) 2018 Natália C. C. de A. Fernandes, Juliana M. Guerra, Rodrigo A. Ressio, Cinthya Cirqueira Borges, Silvia D’Andretta Iglezias, Cristina T. Kanamura, Josué Díaz Delgado, Patricia Sousa Gonçalves, Filipe Onishi Nagamori, Marcelo Sansone, Isis Paixão de Jesus Rizkallah, Mariane Ingara de Moraes Costa, Simone Guimarães de Jesus, Silvana Mello, Júlia de Carvalho, Rosângela Araujo, Magda Almeida Montalvão, Rosemeire Oliveira Lima Rodrigues, Leonardo José Tadeu Araújo, Sônia Maria Pereira de Oliveira, José Luiz Catão Dias
This work is licensed under a Creative Commons Attribution 4.0 International License.
