Palavras-chave
estafilococos coagulase negativa
infecção do trato urinário
resistência à novobiocina
Como Citar
Resumo
O meio de cultura NNS, constituído de púrpura de bromocresol, nitrato de potássio, sacarose e novobiocina, foi utilizado na diferenciação de Staphylococcus saprophyticus isolados de urina. Na formulação do meio NNS, a resistência à novobiocina, característica normalmente utilizada no diagnóstico presuntivo de Staphylococcus saprophyticus, foi associada a sua capacidade de utilizar a sacarose e à ausência da enzima nitrato-redutase, visando a diferenciação deste microorganismo dos demais estafilococos coagulase negativa, inclusive os igualmente resistentes à novobiocina (Staphylococcus cohnii, Staphylococcus xylosus e Staphylococcus sciuri). Os Staphylococcus cohnii não utilizam a sacarose (88% das cepas), enquanto os Staphylococcus xylosus e Staphylococcus sciuri reduzem nitrato a nitrito em 100 e 80% das cepas, respectivamente. De 74 cepas de estafilococos coagulase negativa isoladas de urina, 49 (66,2%) cepas de Staphylococcus saprophyticus foram diferenciadas pelo meio NNS, tendo sido obtidos resultados de valor predicativo de resultado positivo de 100%, sensibilidade de 95,9%, especificidade de 100% e eficiência de 97,2%. As cepas foram posteriormente caracterizadas ao nível de espécie, segundo esquema proposto por KLOOS e SCHLEIFER, quando se obteve 66,2% de Staphylococcus saprophyticus, 13,5% de Staphylococcus simulans, 8,1% de Staphylococcus epidermidis, 5,4% de Staphylococcus haemolyticus, 5,4% de Staphylococcus hominis e 1,4% de Staphylococcus SP...
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Este trabalho está licenciado sob uma licença Creative Commons Attribution 4.0 International License.
Copyright (c) 1989 Maria Lúcia Cecconi Tondella, Cláudio Tavares Sacchi, Sônia Shizue Okita Buschinelli, Silvana Tadeu Casagrande, Maria Cristina de Cunto Brandileone, Lucimar Gonçalves Milagres