Palabras clave
Staphylococcus saprophyticus
estafilococos coagulase negativa
infecção do trato urinário
resistência à novobiocina
estafilococos coagulase negativa
infecção do trato urinário
resistência à novobiocina
Cómo citar
1.
Tondella MLC, Sacchi CT, Buschinelli SSO, Casagrande ST, Brandileone MC de C, Milagres LG. Utilização do meio de cultura Novobiocina-Nitrato-Sacarose (NNS) no diagnóstico presuntivo de Staphylococcus saprophyticus. Rev Inst Adolfo Lutz [Internet]. 29 de diciembre de 1989 [citado 25 de noviembre de 2024];49(2):137-43. Disponible en: https://periodicos.saude.sp.gov.br/RIAL/article/view/36943
Resumen
O meio de cultura NNS, constituído de púrpura de bromocresol, nitrato de potássio, sacarose e novobiocina, foi utilizado na diferenciação de Staphylococcus saprophyticus isolados de urina. Na formulação do meio NNS, a resistência à novobiocina, característica normalmente utilizada no diagnóstico presuntivo de Staphylococcus saprophyticus, foi associada a sua capacidade de utilizar a sacarose e à ausência da enzima nitrato-redutase, visando a diferenciação deste microorganismo dos demais estafilococos coagulase negativa, inclusive os igualmente resistentes à novobiocina (Staphylococcus cohnii, Staphylococcus xylosus e Staphylococcus sciuri). Os Staphylococcus cohnii não utilizam a sacarose (88% das cepas), enquanto os Staphylococcus xylosus e Staphylococcus sciuri reduzem nitrato a nitrito em 100 e 80% das cepas, respectivamente. De 74 cepas de estafilococos coagulase negativa isoladas de urina, 49 (66,2%) cepas de Staphylococcus saprophyticus foram diferenciadas pelo meio NNS, tendo sido obtidos resultados de valor predicativo de resultado positivo de 100%, sensibilidade de 95,9%, especificidade de 100% e eficiência de 97,2%. As cepas foram posteriormente caracterizadas ao nível de espécie, segundo esquema proposto por KLOOS e SCHLEIFER, quando se obteve 66,2% de Staphylococcus saprophyticus, 13,5% de Staphylococcus simulans, 8,1% de Staphylococcus epidermidis, 5,4% de Staphylococcus haemolyticus, 5,4% de Staphylococcus hominis e 1,4% de Staphylococcus SP...
Citas
1. ANDERSON, J.D.; CLARKE, A.M.; ANDERSON, M.E.; ISAAC RENTON, 1.L., & McLOUGHLIN, M.G. - Urinary tract infection due to Staphyl ococcus saprophyticus biotype 3. Cano medo Assoc. J.; 124: 415-8, 1981.
2. BAILLEY, R.R. - Significance of coagulase negative St aph yl o c o cc us in urine. J. infect, Dls., 127: 179-82, 1973.
3. BAILEY, W.R. & SCOTI, E.G. - Diagnostic microbiology, St. Louis, C.V. Mosky Co.; 1966.
4. BAUER, A.W.; KIRBY, W.M.M.; SHERRIS, I.C. & TURCK, M. - Antibiotic suceptibi1ity resting by a standardized single disk method. Amer. J. clin.
Pathol., 45: 493-6, 1966.
5. CARPENTER, C.M.; SUHRLAND, L.G. & MORRISON, M. The oxalate salt of paminodimethylamine, an improved reagent for the oxidase test. Science , 1105: 649, 1947.
6. CRISTENSEN, G.D.; PARISI, I.T.; BISNO, A.L.; SltvlPSON, W.A. & BEACHEY, E,H. - Characterization of clinically significant strains of coagulase-negative staphylocoocci. J. clin, Microbiol., 18: 258-69, 1983.
7. COW AN, S.T. & STEEL, K.J. - Manual for the identification of medica! bacteria. Cambridge University Press, 1975. p. 167.
8. DIFCO LABORATORIES - Difco manual: medios de cultura des hi drat ados y reactions para microbiologia. 10' ed. Detroit, Mich., Difco, 1984. p. 263.
9. FLEURETTE, J. & BRUN, Y. - Rôlc en pathologie humaine de Staphylococcus epidermidls, etude. de 120 souches iisolées des 53 malades. Med. Mal. infect., 2:299-308, 1972.
10. GALEN, R.S. & GAMBINO, S.R. - Beyond normality: the predictive value and efficiency of medical diagnos es, New York, John Wiley, 1975. p. 115-6.
11. GILL, V.I.; SELEPAK, S.T. & WILLIAMS, E.C. Species identification and antibiotic susceptibilities of coagulase-negative staphylococci isolated from clinical specimes .. J. clin, Microbiol., 18 (6):1314-9, 1983.
12. GILLESPIE, W.A.; SELLIN, M.; GILL" P.; STEPHENS, M.; TUCKWELL, L.A. & HILTON, A.L.- Urinary tract infections in young women with special reference to Staphylococcus saprophyticus. J. clin, Pathol., 31.
13. GOLDSTEIN, J.; SCHULMAN, R.; KELLEY, E.; McKINLEY, G. & FUNG, J. - Effect of different media on determination of novobiocin resistence for differenciation of coagulase-negative staphylococci. J. clin, Microbioi.; 18:592-5 1983.
14. HEBERT, G.A.; COOKSEY, R.C.; CLARK x.c., HILL, B.C.; JARVIS, W.R. & THORNSBERRY, C. - Biotyping coagulase negative staphylococci. J.
clin. Microbiol., 26(10):1950-6, 1988.
15. HEBERT, G.A.; CROWDER, C.G.; HANCOCK, G.A.; JARVIS, W.R. & THORNSBERRY, C. - Characteristics of coagulase-negative that help differentiate these species and other membcrs of lhe family Micr oco ccaceae, J. clin, Microbiol., 26(10):1939-49, 1988.
16. INSTITUT PASTEUR - Milieux et Réactifs de Laboratoire Pasteur, Paris, Inst. Pasteur, 1978. p. 120, 141, 186.
17. KLOOS, W.E.; TORNABENET, G. & SCHLEIFER, K.H. - Isolation and characterization of micrococci from human skin, including two new species: Micrococcus lylae and Micrococcus kristinae.Lnt, J. syst. Bacteriol.; 24(1):79-101, 1974.
18. KLOOS, W.E. & SCHLEIFER, K.H. - Simplified scheme for routine identification of human Staphylococcus species. J. clin. Microbiol., 1(1): 82-8, 1975.
19. KLOOS, W,E.; SCHLEIFER, K.H. & SMITH R.F. Characterization of Staphylococcus sciuri sp. nov and its subspecies. Int. J. syst. Bacteriol..; 26:(1) 22-37, 1976.
20. KLOOS, W.E. & WOLFSHOHL, J.F. - Identification of Staphylococcus specics with the API STAPHIDENT system. J. clin: Microbiol.; 16(3):50916, 1982.
21. LA VERDIERE, M.; PETERSON, P.K.; VERHOEF, J.; WILLIAMS, D.N. & SABA TH L.D. - In vitro activity of cephalosporins against methicillin resistant, coagulase negative staphylococci. J. infect. Dis., 137:245-50, 1978.
22. MAC FADDIN, J.F. - Biochimical test for identificatlon of medical bactéria 2 ND, Baltimore & WILKINS, 1980. p. 51-8.
23. MARRIE, T.J.; KW AN, c., MOBLE, M.A.; WEST, A. & DUFFIELD, L. - Staphylococcus saprophyticus as a cause of urinary tract infections J. clln, Microbiol., 16(3):427-31, 1982.
24. MENDES, C.M.F.; GOES SIQUEIRA, L.F. & FRANCISCO, W. - Rapid automated identification of novobiocin resistent, coagulase-negative staphylococci. J. clin, Microbiot., 22.(2):316-7, 1987.
25. NICOLLE, L.E.; HOBAN, S.A. & HARDING, G.K.M. - Caracterization of coagulase-negative staphylococci from urinary tract specimes. J. clin. Microbiol.17(2):267-71, 1983.
26. PICKETT, D.A. & WELCH, D.F. - Recognition of Staphylococcus saprophyticus in urine cultures by screening colonies for production of phosphatase, J. clin. Microbiol., 21(3):310-3, 1985.
27. SACCHI, C.T.; TONDELLA, M.L.C.; BRANDILEONE, M.e.C.; MELLES, C.E.A. & PAULA, M.D.N. - Corynebacterium diphtheriae isolada de sangue. Rev, Inst. Adolfo Lutz, 45(1/2):73-9, 1985.
28. SCHLEIFER, K.H . .& KLOOS, W.E. - Isolation and characterization of staphylococci from human skin. r. Amended description of Staphylococcus epidemills and Staphylococcus saprophyticus and discriptions of three new species: Staphylococcus cohnii, Staphylococcus haemolyt icus and Staphylococcus xylosus.Int. J. syst. Bacteriol., 25:50-
61, 1975.
29. SCHLEIFER, K.H. & KLOOS, W.E. - A simple test system for lhe separation of staphylococci from micrococci. J. clin, Microbiol., 1(3):337-8, 1975.
30. SEWELL, e.M.; CLARRIDGE, J.E.; YOUNG, E.J.; & GUTI-IRIE, R.K. - Clinical significance of coagulase-negative staphylococci. J. clin. Mlcr obiol.,
16:236-9, 1982.
31. STEVENS, D.L.; JONES, e. - Use of trealosemanitol-phosphatase agar to differentiate Staphylococcus ep idermidls and Staphylococcus sapr oph yt icus from other coagulase-negative Staphylococcus. J. clin, Microbiol.20(5):977-80, 1984.
32. Subcommittee on taxonomy of staphylococci and micrococci recomendation. Int. Buli. Bacteriol, Nomencl., 15:109-10, 1965.
33. WILKINSON, B.J.; MAXWELL, S. & SCHAUS, S.M. - Classification and characteristics of coagulase-negative, methicillin-resistant staphylococci. J. clin, Mlcrobiol., 12:161-6, 1980.
2. BAILLEY, R.R. - Significance of coagulase negative St aph yl o c o cc us in urine. J. infect, Dls., 127: 179-82, 1973.
3. BAILEY, W.R. & SCOTI, E.G. - Diagnostic microbiology, St. Louis, C.V. Mosky Co.; 1966.
4. BAUER, A.W.; KIRBY, W.M.M.; SHERRIS, I.C. & TURCK, M. - Antibiotic suceptibi1ity resting by a standardized single disk method. Amer. J. clin.
Pathol., 45: 493-6, 1966.
5. CARPENTER, C.M.; SUHRLAND, L.G. & MORRISON, M. The oxalate salt of paminodimethylamine, an improved reagent for the oxidase test. Science , 1105: 649, 1947.
6. CRISTENSEN, G.D.; PARISI, I.T.; BISNO, A.L.; SltvlPSON, W.A. & BEACHEY, E,H. - Characterization of clinically significant strains of coagulase-negative staphylocoocci. J. clin, Microbiol., 18: 258-69, 1983.
7. COW AN, S.T. & STEEL, K.J. - Manual for the identification of medica! bacteria. Cambridge University Press, 1975. p. 167.
8. DIFCO LABORATORIES - Difco manual: medios de cultura des hi drat ados y reactions para microbiologia. 10' ed. Detroit, Mich., Difco, 1984. p. 263.
9. FLEURETTE, J. & BRUN, Y. - Rôlc en pathologie humaine de Staphylococcus epidermidls, etude. de 120 souches iisolées des 53 malades. Med. Mal. infect., 2:299-308, 1972.
10. GALEN, R.S. & GAMBINO, S.R. - Beyond normality: the predictive value and efficiency of medical diagnos es, New York, John Wiley, 1975. p. 115-6.
11. GILL, V.I.; SELEPAK, S.T. & WILLIAMS, E.C. Species identification and antibiotic susceptibilities of coagulase-negative staphylococci isolated from clinical specimes .. J. clin, Microbiol., 18 (6):1314-9, 1983.
12. GILLESPIE, W.A.; SELLIN, M.; GILL" P.; STEPHENS, M.; TUCKWELL, L.A. & HILTON, A.L.- Urinary tract infections in young women with special reference to Staphylococcus saprophyticus. J. clin, Pathol., 31.
13. GOLDSTEIN, J.; SCHULMAN, R.; KELLEY, E.; McKINLEY, G. & FUNG, J. - Effect of different media on determination of novobiocin resistence for differenciation of coagulase-negative staphylococci. J. clin, Microbioi.; 18:592-5 1983.
14. HEBERT, G.A.; COOKSEY, R.C.; CLARK x.c., HILL, B.C.; JARVIS, W.R. & THORNSBERRY, C. - Biotyping coagulase negative staphylococci. J.
clin. Microbiol., 26(10):1950-6, 1988.
15. HEBERT, G.A.; CROWDER, C.G.; HANCOCK, G.A.; JARVIS, W.R. & THORNSBERRY, C. - Characteristics of coagulase-negative that help differentiate these species and other membcrs of lhe family Micr oco ccaceae, J. clin, Microbiol., 26(10):1939-49, 1988.
16. INSTITUT PASTEUR - Milieux et Réactifs de Laboratoire Pasteur, Paris, Inst. Pasteur, 1978. p. 120, 141, 186.
17. KLOOS, W.E.; TORNABENET, G. & SCHLEIFER, K.H. - Isolation and characterization of micrococci from human skin, including two new species: Micrococcus lylae and Micrococcus kristinae.Lnt, J. syst. Bacteriol.; 24(1):79-101, 1974.
18. KLOOS, W.E. & SCHLEIFER, K.H. - Simplified scheme for routine identification of human Staphylococcus species. J. clin. Microbiol., 1(1): 82-8, 1975.
19. KLOOS, W,E.; SCHLEIFER, K.H. & SMITH R.F. Characterization of Staphylococcus sciuri sp. nov and its subspecies. Int. J. syst. Bacteriol..; 26:(1) 22-37, 1976.
20. KLOOS, W.E. & WOLFSHOHL, J.F. - Identification of Staphylococcus specics with the API STAPHIDENT system. J. clin: Microbiol.; 16(3):50916, 1982.
21. LA VERDIERE, M.; PETERSON, P.K.; VERHOEF, J.; WILLIAMS, D.N. & SABA TH L.D. - In vitro activity of cephalosporins against methicillin resistant, coagulase negative staphylococci. J. infect. Dis., 137:245-50, 1978.
22. MAC FADDIN, J.F. - Biochimical test for identificatlon of medical bactéria 2 ND, Baltimore & WILKINS, 1980. p. 51-8.
23. MARRIE, T.J.; KW AN, c., MOBLE, M.A.; WEST, A. & DUFFIELD, L. - Staphylococcus saprophyticus as a cause of urinary tract infections J. clln, Microbiol., 16(3):427-31, 1982.
24. MENDES, C.M.F.; GOES SIQUEIRA, L.F. & FRANCISCO, W. - Rapid automated identification of novobiocin resistent, coagulase-negative staphylococci. J. clin, Microbiot., 22.(2):316-7, 1987.
25. NICOLLE, L.E.; HOBAN, S.A. & HARDING, G.K.M. - Caracterization of coagulase-negative staphylococci from urinary tract specimes. J. clin. Microbiol.17(2):267-71, 1983.
26. PICKETT, D.A. & WELCH, D.F. - Recognition of Staphylococcus saprophyticus in urine cultures by screening colonies for production of phosphatase, J. clin. Microbiol., 21(3):310-3, 1985.
27. SACCHI, C.T.; TONDELLA, M.L.C.; BRANDILEONE, M.e.C.; MELLES, C.E.A. & PAULA, M.D.N. - Corynebacterium diphtheriae isolada de sangue. Rev, Inst. Adolfo Lutz, 45(1/2):73-9, 1985.
28. SCHLEIFER, K.H . .& KLOOS, W.E. - Isolation and characterization of staphylococci from human skin. r. Amended description of Staphylococcus epidemills and Staphylococcus saprophyticus and discriptions of three new species: Staphylococcus cohnii, Staphylococcus haemolyt icus and Staphylococcus xylosus.Int. J. syst. Bacteriol., 25:50-
61, 1975.
29. SCHLEIFER, K.H. & KLOOS, W.E. - A simple test system for lhe separation of staphylococci from micrococci. J. clin, Microbiol., 1(3):337-8, 1975.
30. SEWELL, e.M.; CLARRIDGE, J.E.; YOUNG, E.J.; & GUTI-IRIE, R.K. - Clinical significance of coagulase-negative staphylococci. J. clin. Mlcr obiol.,
16:236-9, 1982.
31. STEVENS, D.L.; JONES, e. - Use of trealosemanitol-phosphatase agar to differentiate Staphylococcus ep idermidls and Staphylococcus sapr oph yt icus from other coagulase-negative Staphylococcus. J. clin, Microbiol.20(5):977-80, 1984.
32. Subcommittee on taxonomy of staphylococci and micrococci recomendation. Int. Buli. Bacteriol, Nomencl., 15:109-10, 1965.
33. WILKINSON, B.J.; MAXWELL, S. & SCHAUS, S.M. - Classification and characteristics of coagulase-negative, methicillin-resistant staphylococci. J. clin, Mlcrobiol., 12:161-6, 1980.
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Derechos de autor 1989 Revista del Instituto Adolfo Lutz
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