Chemical composition and antibacterial activity of essential oils from Cymbopogon winterianus (citronella), Eucalyptus paniculata (eucalyptus) and Lavandula angustifolia (lavender)
PDF (Português (Brasil))

Keywords

volatile oils
antimicrobials
natural products
medicinal plants

How to Cite

1.
Silveira SM da, Cunha Júnior A, Scheuermann GN, Secchi FL, Verruck S, Krohn M, Vieira CRW. Chemical composition and antibacterial activity of essential oils from Cymbopogon winterianus (citronella), Eucalyptus paniculata (eucalyptus) and Lavandula angustifolia (lavender). Rev Inst Adolfo Lutz [Internet]. 2012 Mar. 1 [cited 2024 Jul. 22];71(3):471-80. Available from: https://periodicos.saude.sp.gov.br/RIAL/article/view/32453

Abstract

The chemical composition of essential oils derived from three medicinal plant species growing in the South of Brazil: Cymbopogon winterianus (citronella), Eucalyptus paniculata (eucalyptus), and Lavandula angustifolia (lavender) was determined, and their antimicrobial activities were evaluated against 11 bacterial species, including those related to foodborne and human pathogens. The essential oils were obtained by steam distillation, and its chemical composition was analyzed by GC and GC/MS. Antimicrobial activity was screened by disc-diffusion assay. MIC and MBC of the sensitive strains were determined by microdilution methodology. The main components of lavender and citronella essential oils were the oxygenated monoterpenes, and the monoterpene hydrocarbons in eucalyptus oil. Citronella oil showed the lowest MIC and MBC values on the majority of the tested bacteria, and the MIC and MBC values against Yersinia enterocolitica were of 0.075 and 0.31 mg/mL, respectively. The lavender oil was active against Escherichia coli and Salmonella Typhimurium, and the eucalyptus inhibited the growth of Pseudomonas aeruginosa. This study showed that the evaluated essential oils might be potentially used as natural antimicrobial agents.
https://doi.org/10.53393/rial.2012.v71.32453
PDF (Português (Brasil))

References

1. Hamburger M, Hostettmann K. Bioactivity in plants: the link between phytochemistry and medicine. Phytochem. 1991;30(12):3864-74.

2. Silva Junior AA. Essentia herba – Plantas bioativas. Florianópolis (SC): Epagri; 2003.

3. Militello M, Settani L, Aleo A, Mammina C, Moschetti G, Giammanco GM, et al. Chemical composition and antibacterial potential of Artemisia arborescens L. essential oil. Curr Microbiol. 2011;62:1274-81.

4. Rahman A, Kang SC. Inhibition of foodborn pathogens and spoiling bacteria by essential oil and extracts of Erigeron ramosus(Walt.) BSPJ Food Saf. 2009;29:176-89.

5. Viuda-Martos M, El-Nasser A, El Gendy GS, Sendra E, Fernández-López J, El Razik KAA, et al. Chemical composition and antioxidant and anti-Listeria activities of essential oils obtained from some Egyptian plants. J Agric Food Chem. 2010;58:9063-70.

6. Conner DE. Naturally occurring compounds. In: Davidson P, Branen AL (Eds.). Antimicrobials in foods. Nova York: Marcel Dekker; 1993. p. 441-68.

7. Oussalah M, Caillet S, Saucier L, Lacroix M. Inhibitory effects of selected plant essential oils on the growth of four pathogenic bacteria: E. coli O157:H7, Salmonella Typhimurium, Staphylococcus aureus and Listeria monocytogenes. Food Control. 2007;18(5):414-20.

8. Shasany AK, Lal RK, Patra NK, Darokar MP, Garg A, Kumar S, et al. Phenotypic and RAPD diversity among Cymbopogon winterianus Jowitt accessions in relation to Cymbopogon nardusRendle. Genet Resour Crop Evol. 2000;47:553-9.

9. Nerio LS, Olivero-Verbel J, Stashenko E. Repellent activity of essential oils: a review. Biores Technol. 2010;101:372-8.

10. Mendonça FAC, da Silva KFS, dos Santos KK, Ribeiro Júnior KAL, Sant’Ana AEG. Activities of some Brazilian plants against larvae of the mosquito Aedes aegypti. Fitoterapia. 2005;76:629-36.

11. Duarte MCT, Leme EE, Delarmelina C, Soares AA, Figueira GM, Sartoratto A. Activity of essential oils from Brazilian medicinal plants on Escherichia coli. J Ethnopharmacol. 2007;111:197-201.

12. Oliveira MMM, Brugnera DF, Cardoso MG, Guimarães LGL, Piccoli RH. Rendimento, composição química e atividade antilisterial de oleos essenciais de espécies de Cymbopogon. Rev Bras Plantas Med. 2011;13(1):8-16.

13. Cavanagh HMA, Wilkinson JM. Biological activities of lavender essential oil. Phytother Res. 2002;16:301-8.

14. Roller S, Ernest N, Buckle J. The antimicrobial activity of high necrodane and other lavender oils on methicilin-sensitive and -resistant Staphylococcus aureus (MSSA and MRSA). J Altern Complement Med. 2009;15(3):275-9.

15. Daferera DJ, Ziogas BN, Polissiou MG. GC-MS analysis of essential oils from some greek aromatic plants and their fungitoxicity on Penicillium digitatum. J Agric Food Chem. 2000;48:2576-81.

16. Cavanagh HMA, Wilkinson JM. Lavender essential oil: a review. Austr Infect Control. 2005;10(1):35-7.

17. Estanislau AA, Barros FAZ, Peña AP, Santos SC, Ferri PH, Paula JR. Composição química e atividade antibacteriana dos óleos essenciais de cinco espécies de Eucalyptus cultivadas em Goiás. Rev Bras Farmacogn. 2001;11(2):95-100.

18. Gilles M, Zhao J, Na M, Agboola S. Chemical composition and antimicrobial properties of essential oils of three Australian Eucalyptus species. Food Chem. 2010;119:731-7.

19. Harborne SB, Baxter H. Phytochemical Dictionary. Londres: Taylor and Francis; 1995.

20. Sartorelli P, Marquioreto AD, Amaral-Baroli A, Lima MEL, Moreno PRH. Chemical composition and antimicrobial activity of the essential oils from two species of Eucalyptus. Phytother Res. 2007;21:231-3.

21. Adams RP. Identification of Essential Oil Components by Gas Chromatographic/Quadrupole Mass Spectrometry. Carol Stream (IL): Allured; 2001.

22. NCCLS. The National Committee for Clinical Laboratory Standards. The Performance Standards for Antimicrobial Disk Susceptibility Tests; Approved Standard. 8ª ed. NCCLS document M2-A8. Wayne (PA): NCCLS; 2003.

23. Carovic-Stanko K, Orlic S, Politeo O, Strikic F, Kolak I, Milos M, et al. Composition and antibacterial activities of essential oils of seven Ocimum taxa. Food Chem.2010;119:196-201.

24. NCCLS. The National Committee for Clinical Laboratory Standards. The Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria that Grow Aerobically; Approved Standard. 6ª ed. NCCLS document M7-A6. Wayne (PA): NCCLS; 2003.

25. Smânia A Jr., Monache FD, Smânia EF, Gil ML, Benchetrit LC, Cruz FS. Antibacterial activity of a substance produced by the fungus Pycnoporus sanguineus (Fr.) Murr. J Ethnopharmacol. 1995;45:177-81.

26. Holetz FB, Pessini GL, Sanches NR, Cortez DAG, Nakamura CV, Dias Filho BP. Screening of some plants used in the Brazilian folk medicine for the treatment of infectious diseases. Mem Inst Oswaldo Cruz. 2002;97(7):1027-31.

27. Ayres MCC, Brandão MS, Vieira-Júnior GM, Menor JCAS, Silva HB, Soares MJS, et al. Atividade antibacteriana de plantas úteis e constituintes químicos da raiz de Copernicia prunifera. Rev Bras Farmacogn. 2008;18(1):90-7.

28. Scherer R, Wagner R, Duarte MCT, Godoy HT. Composição e atividades antioxidante e antimicrobiana dos óleos essenciais de cravo-da-índia, citronela e palmarosa. Rev Bras Plantas Med. 2009;11(4):442-9.

29. Kim J, Marshall MR, Wei C. Antibacterial activity of some essential oil components against five foodborne pathogens. J Agric Food Chem. 1995;43:2839-45.

30. Burt S. Essential oils: their antibacterial properties and potential applications in foods – a review. Int J Food Microbiol. 2004;94:223-53.

31. Russel AD. Mechanisms of bacterial resistance to non-antibiotics: food additives and food and pharmaceutical preservatives. J Appl Microbiol. 1991;71:191-201.

32. Sokovic M, Marin PD, Brkic D, Van Griensven LJLD. Chemical composition and antibacterial activity of essential oils of ten aromatic plants against human pathogenic bacteria. Food. 2008;1(2):220-6.

33. Hajhashemi V, Ghannadi A, Sharif B. Anti-inflammatory and analgesic properties of the leaf extracts and essential oil of Lavandula angustifolia Mill. J Ethnopharmacol. 2003;89:67-71.

34. Van Vuuren SF, Viljoen AM. Antimicrobial activity of limonene enantiomers and 1,8-cineole and in combination. Flavour Fragr J. 2007;22:540-4.

35. Alexopoulos A, Kimbaris AC, Plessas S, Mantzourani I, Theodoridou I, Stavropoulou E. Antibacterial activities of essential oils from eight Greek aromatic plants against clinical isolates of Staphylococcus aureus. Anaerobe. 2011;17:399-402.

36. Azar PA, Torabbeigi M, Sharifan A, Tehrani MS. Chemical composition and antibacterial activity of the essential oil of Lavandula angustifolia isolated by solvent free microwave assisted extraction and hydrodistillation. J Food Biosci Technol. 2011;1:19-24.

37. Peter KV (Ed.). Handbook of herbs and spices. V.2. Cambridge: Woodhead Publishing Limited; 2004.

38. Cox SD, Mann CM, Markham JL, Gustafson JE, Warmington JR. Determining the antimicrobial actions of tea tree oil. Molecules. 2001;6:87-91.

39. Oyedemi SO, Okoh AI, Mabinya LV, Pirochenva G, Afolayan AJ. The proposed mechanism of bactericidal action of eugenol, α-terpineol and γ-terpinene against Listeria monocytogenes, Streptococcus pyogenes,Proteus vulgaris and Escherichia coli. Afr J Biotechnol. 2009;8(7):1280-6.

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Copyright (c) 2012 Instituto Adolfo Lutz Journal

Downloads

Download data is not yet available.