Keywords
bee pollen
HPLC analysis
statistical experimental design
How to Cite
Abstract
The analytical methodology for the determination of four flavonols: myricetin (M), quercetin (Q), kanferol (K), isorhamnetin (I); and two flavones: luteolin (L) and apigenin (A) in samples of dehydrated bee pollen produced in three Brazilian states: Bahia (BA), São Paulo (SP) and Santa Catarina (SC) was optimized. Central Composite Rotational Design was used to investigate the effects of HCl concentration and hydrolysis time on the concentration of each flavonoid. The optimum condition found for extraction/hydrolysis of the flavonoids studied was 1.0M HCl/30 minutes. The best separation of the flavonoids was achieved with a reverse phase Symmetry C18 column with methanol:tetrahydrofuran:water (26:57:17) acidified with 0.3% formic acid as mobile phase in isocratric elution (HPLC). The standard curves presented coefficients of correlation superior to 0.99. The limits of detection were 1.04, 0.88, 0.89, 1.64, 0.82 and 1.19 μg/mL for L, Q, A, I and K, respectively.
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