Abstract
Cyanobacteria are responsible for the production of cyanotoxins, and once accumulated, it might cause serious harm to the human and animals health. Microcystins are the most common type of cyanotoxins and they cause liver tumor. The Mundau reservoir, located in the municipality of Garanhuns-PE, was the selected site for presenting historical blooms of microcystins-producing cyanobacteria. This study analyzed the occurrence of microcystins in raw water samples from the Mundau reservoir by using Enzyme Linked Immunosorbent Assay (ELISA) and High Performance Liquid Chromatography (HPLC) methodologies. During the consecutive fifteen months, water samples were collected in duplicate at the intake point of this water source, and they were analyzed by both ELISA and HPLC techniques. The presence of microcystins was detected in 100 % of analyzed samples, confirming the relevance of performing the microcystins monitoring in public water supplies. And as well as the Mundau river, various water sources of Pernambuco show cyanobacterial blooms, which might be toxic. The techniques used in this study were implemented in the Central Laboratory of Public Health of Pernambuco (LACEN-PE), and they showed to be a relevant tools as a complementary methodology to the cyanobacteria counting assay for providing preventive subsidies to the health surveillance.References
1. Libânio M. Fundamentos de qualidade e tratamento de água. Campinas, São Paulo: Átomo; 2008.
2. Sanches SM, Vieira EM, Prado EL, Beneti F, Takayanagui AMM. Estudo da presença da toxina microcistina - LR em água utilizada em clínica de hemodiálise e validação de um método analítico. Ecl Quim.2007;32(4):43-8.
3. Cerione EM, Cavagioni MG, Breir TB, Barrella W, Almeida VP. Levantamento de espécies de algas planctônicas e análise da água do lago do Zoológico Quinzinho de Barros, Sorocaba (SP). REB.2008;1(2):18-27.
4. Codd GA, Azevedo SMFO, Bagchi SN, Burch MD, Carmichael WW, Harding WR, Utiklen HC. Cyanonet - A global network for cyanobacterial bloom and toxin risk management. Initial situation assessment and recommendations. IHP-VI Technical Document in Hidrology,n. 76. Unesco, Paris, Working Series SC 2005/WS/55, 2005.
5. Oberholster PJ, Myburghb JG, Govender D, Bengis R, Botha AM et al. Identification of toxigenic Microcystis strains after incidents of wild animal mortalities in the Kruger National Park, South Africa. Ecotoxicol Environm Saf.2009;72:1177-82.
6. Fernandes VO, Cavati B, Oliveira LB, Souza BDA. Ecologia de cianobactérias: fatores promotores e consequências das florações. Oecol Bras.2009;13(2):247-58.
7. Carneiro TG, Leite F. Cianobactérias e suas toxinas. Rev Analyt.2008; 32:36-41.
8. Abramsson-Zetterberg LU, Sundh R, Mattsson R. Cyanobacterial extracts and microcystin-LR are inactive in the micronucleus assay in vivo and in vitro. Mutat Res.2010;699(1-2):5-10.
9. Nybom SNK, Dziga D, Heikkilä J, Kull T, Salminen SJ, Meriluoto JAO. Characterization of mycrocistin-LR removal process in the presence of probiotic bacteria. Toxicon.2012;59(1):171-81.
10. Carmichael WW. The toxins of cyanobacteria. Sci Am.1994;270:78-86.
11. Falconer IR. Tumor promotion and liver injury caused by oral consumption of cyanobacteria. Environm Toxicol.1991;6:177-84.
12. Meng G, Sun Y, Fu W, Guo Z, Xu L. Microcystin-LR induces cytoskeleton system reorganization through hyperphosphorylation of tau and HSP27 via PP2A inhibition and subsequent activation of the p38 MAPK signaling pathway in neuroendocrine (PC12) cells. Toxicology.2011;290:218-29.
13. Brasil. Ministério da Saúde. Portaria n° 2914, de 12 de dezembro de 2011. Dispõe sobre os procedimentos de controle e de vigilância da qualidade da água para consumo humano e seu padrão de potabilidade.Diário Oficial [da] República Federativa do Brasil. Brasília, DF, 14 dez. 2011. Seção 1. p. 39.
14. Chorus I, Bartram J. Toxic Cyanobacteria in Water: A Guide to their Public Health Consequences, Monitoring and Management. World Health Organization. London: E & FN Spon;1999. 416pp.
15. Brasil. Ministério da Saúde. Portaria n° 518, de 25 de março de 2004. Estabelece os procedimentos e responsabilidades relativos ao controle e vigilância da qualidade da água para consumo humano e seu padrão de potabilidade, e dá outras providências. Diário Oficial [da] República Federativa do Brasil. Brasília, DF, 26 mar. 2004.
16. Sant’Anna CL, Azevedo MTP, Werner VR, Dogo, CR, Rios FRC. Review of toxic species of Cyanobacteria in Brazil.Algol Stud.2008;126:215-65.
17. Molica RJR, Onodera H, Garcia C, Rivas M, Adrendo D, Nascimento S, et al. Toxins in the freshwater cyanobacterium Cylindrospermopsis raciborskii (Cyanophyceae) isolated from Tabocas reservoir in Caruaru, Brazil, including demonstration of a new saxitoxin analogue. Phycologia.2002; 41:606-11.
18. Azevedo SMFO. Toxic cyanobacteria and the Caruaru tragedy. IV Simpósio da Sociedade Brasileira de Toxicologia; 1996; p.84.
19. Jochimsen EM, Carmichael WW, An JS, Cardo DW, Cookson ST, Holmes CE, et al. Liver failure and death after exposure to microcystin toxins at a hemodialysis center in Brazil. N Engl J Med.1998;13(338):873-78.
20. Chellappa NT, Chellappa SL, Chellappa S. Harmful Phytoplankton Blooms and Fish Mortality in a eutrophicated reservoir of Northeast Brazil. Braz Arch Biol Technol.2008;51(4):833-41.
Agência Pernambucana de Águas e Clima. Rio Mundaú [Internet]. Recife [acesso em 20 dez.2012]. Disponível em: http://www.apac.pe.gov.br/pagina.php?page_id=5&subpage_id=19
22. Secretaria de Recursos Hídricos de Pernambuco (SRH). Plano Estadual de Recursos Hídricos do Estado de Pernambuco. Documento Síntese, Recife, 2000. 267pp.
23. Utermöhl H. Zur vervollkommer der quantitativen phytoplankton methodik. Mitt Int Ver. Theor Angew Limnol.1958;5:567-96.
24. Harada K, Kondo F, Lawton L. Laboratory Analysis of Cyanotoxins. In: Chorus I, Bartram J. Toxic Cyanobacteria in Water: A Guide to their Public Health Consequences, Monitoring and Management. World Health Organization. London: E & FN Spon;1999. p.362-400.
25. Fastner J, Flieger I, Neumann U. Optimised extraction of microcystins from field samples - a comparison of different solvents and procedures. Water Res.1998;32:3177-81.
26. Lawton LA, Edwards C, Codd GA. Extraction and high-performance liquid chromatographic method for the determination of microcystins in raw and treated waters. Analyst.1994;119(7):1525-30.
27. Heo WM, Kim B. The effect of artificial destratification on phytoplankton in a reservoir. Hydrobiologia.2004;524:229-39.
28. Brasil. Resolução nº 357, de 17 de março de 2005. Dispõe sobre a classificação dos corpos de água e diretrizes ambientais para o seu enquadramento bem como estabelece as condições e padrões de lançamento de efluentes, e dá outras providências. Diário Oficial [da] República Federativa do Brasil. Brasília, DF, 18 mar. 2005. Seção 1. p. 53.
29. Mozaz SR, Alda MJL, Barcelo D. Advantages and limitations of on-line solid phase extraction coupled to liquid chromatography-mass spectrometry technologies versus biosensors for monitoring of emerging contaminants in water. J Chromatogr A.2007;1152:97-115.
30. Utkilen H, Gjolme N. Iron-Stimulated Toxin Production in Microcystis aeruginosa. Appl Environ Microbiol.1995;61:797-800.
31. Agência Nacional de Vigilância Sanitária; ANVISA. Resolução n. 899, de 29 de maio de 2003. Guia para Validação de Métodos Analíticos e Bioanalíticos. Diário Oficial [da] República Federativa do Brasil. Brasília, DF, 2 jul. 2003.
32. Instituto Nacional de Metrologia, Normalização e Qualidade Industrial. DOQ-CGCRE-008: Orientações sobre validação de métodos de ensaios químicos. Rio de Janeiro (RJ): INMETRO; 2011. Revisão 4. 20 p.
33. Msagati TAM, Siame BA, Shushu DD. Evaluation of methods for the isolation, detection and quantification of cyanobacterial hepatotoxins. Aquat Toxicol.2006;78:382-97.
34. Wang D, Weston DP, Lydy MJ. Method development for the analysis of organophosphate and pyrethroid insecticides at low parts per trillion levels in water. Talanta.2009;78:345-51.
35. Mekebri A, Blondina GJ, Crane DB. Method validation of microcystins in water and tissue by enhanced liquid chromatography tandem mass spectrometry. J Chromatogr A.2009;1216:3147-55.
36. Rivasseau C, Martins S, Hennion MC. Determination of some physicochemical parameters of microcystins (cyanobacterial toxins) and trace level analysis in environmental samples using liquid chromatography. J Chromatogr A.1998;799:155-69.
37. Bogialli S, Bruno M, Curini R, Di Corcia A, Fanali C, Laganà A. Monitoring algal toxins in lake water by liquid chromatography tandem mass spectrometry. Environ Sci Technol.2006;40:2917-23.
This work is licensed under a Creative Commons Attribution 4.0 International License.
Copyright (c) 2015 Instituto Adolfo Lutz Journal