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Abstract
Extraction and cleanup systems were evaluated for the determination of trichothecenes in corn by gas chromatography with flame ionization detector. The extraction and cleanup method described by Furlong & Soares (1995), combined with the Romer (1986) cleanup column exhibited the best results. The extraction used methanol: 4% potassium chloride (9:1), followed by a clarification with 30% ammonium sulfate, and partition with dichloromethane. The extract was then passed through an alumina:carbon column (2.3:1.9) and eluted with acetonitrile:water (84:16). Trifluoroacetic anhydride in the presence of pyridine was used for derivatization before injection in a gas chromatograph with an ionization detector. The average recoveries were 72% for deoxynivalenol (DON), 88% for diacetoxyscirpenol (DAS) and 87% for toxin T2 (T2). The detection limits were 30 ng/g for DON, 50 ng/g for DAS and 40 ng/g for T2. The average coefficients of variation for spiked samples at the 500 ng/g level were 9.8, 6.3 e 6.6% for DON, DAS, and T2, respectively.
References
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Copyright (c) 2001 Adriana de Q. Oliveira, Lucia M. Valente Soares