Keywords
ELISA
thin-layer chromatography
imunoassay
aflatoxins
ochratoxin A
zearalenone
corn and corn meal
thin-layer chromatography
imunoassay
aflatoxins
ochratoxin A
zearalenone
corn and corn meal
How to Cite
1.
V. Milanez T, Bittar Atui M, A. Lázzari F. Comparison between immunoassay and thin-layer chromatography for determination of aflatoxins, ochratoxin A and zearalenone in corn and com meal. Rev Inst Adolfo Lutz [Internet]. 1998 Jun. 30 [cited 2024 Jul. 22];57(1):65-71. Available from: https://periodicos.saude.sp.gov.br/RIAL/article/view/36623
Abstract
Twotechniqucs, one ofthem using thin-layer chromatography and the other enzyme-Iinked irrununosorbent assay (ELISA) were compared for the detennination of aflatoxins, ochratoxin A and zearalenone. Seven sampIes of com and twenty samp1csof com meal from an industry in Paraná State were analysed for this purpose.The ELISA method was simpler to use, faster, showing higher concentrations than thc thin-layer chromatography mcthod. False positives requiring confirmation of the mycotoxins were observed with the ELISA method.
References
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12. MILLER, J.D. Fungi and mycotoxins in grain: implications for stored product research, J. Stored Prod. Res., 31 (1): 1-16, 1995.
13. MORGAN, M.R.A.; KANG, A.S. & CHAN, H.W.S. Aflatoxin dctermination in peanut butter by enzymelinked immunosorbent assay. J. Sei. Food Agrie. ,37: 908-914,1986.
14. PESTKA, J.1.; ABOUZIED, M.N. & SUTKINO Immunological assays for mycotoxin detection. Food Technology, 49(2): 120-128, 1995.
15. PONS, W.A. High pressure liquid chromatographic detennination of aflatoxins in com. J. Assoe. Off. Anal. Chem., 62: 586-594,1979.
16. PRZYBYLSKI, W. Fonnation ofaflatoxin derivatives on thin-layer chromatographic plates. J. Assoe Off. Ana!. Chem., 58: 163-164, 1975.
17. SCOTT, P.M. Mycotoxins. J. Assoe. Off. Anal. Chem. Intern., 76: 112, 1993.
18. SCOTT, P.M. Recent developments in methods of analysis for mycotoxins in foodstuffs. Trends in Analytieal Chemistry, 12(9): 373-381,1993.
19. SCOTT, P.M.; LAU, P. & KANHERE, S.R. Gas chromatography with electron capture and mass spectrometric detection of deoxynivalenol in wheat and other grains. J Assoe. Off. Anal. Chem., 64: 1364-1371, 1981.
20. SHANNON, G.M.; SHOTWELL, O.L. & KWOLEK, W.F. Extraction and thin Iayer chromatography of . aflatoxin BJ in mixed feeds. J Assoe. Off. Anal. Chem., 66: 582-586, 1983.
21. SOARES, L.V.S.; RODRIGUEZ-AMAYA, D.B. Survey of aflatoxins, ochratoxin A, zearalenone and sterigmatocystin in some Brazilian foods by using multi-toxin thin-layer chromatographic method. J
Assoe. Off. Anal. Chem., 72(1):22-26,1989.
22. WATSON, S.A. & RAMSTAD, P.E. 1987 - Com: Chemistry and Technology. St. Paul, M.N.: American Association o/Cereal Chemists, Inc., 605 p.
23. WORLD HEALTH ORGANIZATION - Mycotoxins . Geneva, WHO, 1979. 127p. (EnvironrnentalHealth Criteria, 11).
2. BRASIL, Leis, decretos, etc.- Resolução nº 34/76 da Comissão Nacional de Normas e Padrões para Alimentos. Diário Oficial, Brasília, 19 jan. 1977. Seção I, pt.I, p. 710. Fixa padrões de tolerância para as aflatoxinas em alimentos.
3. CHAMBON, P.; DANO, S.D.; CHAMBON, R. & GEAHCHAN, A.Rapid determination of aflatoxin Ml milk and milk products by high pcrformance liquid chromatography. J. Chromatogr., 259(2): 372-374,
1983.
4. CHU, F.S.; UENO,I. Production of antibody against aflatoxin Bl' Appl. Environ. Microbiol., 33:11251128,1977.
5. lAR C Working Group 011 the Evaluation of the Carcinogenic Risk of Chemical to mano Lyon, 1975. Some naturally oeeurring substanees. Lyon, IARC, 1976. p.51-72 (IARC Monographs , 10).
6. HONGYO, K.I.; ITOH, Y; HIFUMI, E.; TAKEYASU, A. Comparison of monoclonal antibody-based enzyme-linked immunosorbent assay with thin-layer chromatography and liquid chromatography for aflatoxin Bl determination in naturally contaminated com and mixed feed. J. AOAC Internat., 75(2): 307312,1992.
7. HORWITZ, W.; ALBERT, R.; NESHEIM, S. Reliability of mycotoxin assays - an update. J. AOAC Internat., 76(3): 461-491,1993.
8. LANSDEN,J.A.Detennination of cyclopiazonic acid in peanuts and com by thin layer chromatography. J. Assoe. Off. Anal. Chem., 69: 964-966, 1986.
9. LANSDEN,J.A. Liquid chrornatographic analysis system inpeanuts. for a cyclopiazonic acid. J. Assoe. Off. Anal. Chem., 67: 728-731. 1984.
10. LAZZARI, F.A. 1993. Umidade.fungos e mieotoxinas na qualidade de sementes, grãos e rações. Curitiba, Ed. do Autor, 140p.
11. MERCOSUL\ GMC \ Resolução n 2 56\94 - Regulamento Técnico sobre limites máximos de aflatoxinas.
12. MILLER, J.D. Fungi and mycotoxins in grain: implications for stored product research, J. Stored Prod. Res., 31 (1): 1-16, 1995.
13. MORGAN, M.R.A.; KANG, A.S. & CHAN, H.W.S. Aflatoxin dctermination in peanut butter by enzymelinked immunosorbent assay. J. Sei. Food Agrie. ,37: 908-914,1986.
14. PESTKA, J.1.; ABOUZIED, M.N. & SUTKINO Immunological assays for mycotoxin detection. Food Technology, 49(2): 120-128, 1995.
15. PONS, W.A. High pressure liquid chromatographic detennination of aflatoxins in com. J. Assoe. Off. Anal. Chem., 62: 586-594,1979.
16. PRZYBYLSKI, W. Fonnation ofaflatoxin derivatives on thin-layer chromatographic plates. J. Assoe Off. Ana!. Chem., 58: 163-164, 1975.
17. SCOTT, P.M. Mycotoxins. J. Assoe. Off. Anal. Chem. Intern., 76: 112, 1993.
18. SCOTT, P.M. Recent developments in methods of analysis for mycotoxins in foodstuffs. Trends in Analytieal Chemistry, 12(9): 373-381,1993.
19. SCOTT, P.M.; LAU, P. & KANHERE, S.R. Gas chromatography with electron capture and mass spectrometric detection of deoxynivalenol in wheat and other grains. J Assoe. Off. Anal. Chem., 64: 1364-1371, 1981.
20. SHANNON, G.M.; SHOTWELL, O.L. & KWOLEK, W.F. Extraction and thin Iayer chromatography of . aflatoxin BJ in mixed feeds. J Assoe. Off. Anal. Chem., 66: 582-586, 1983.
21. SOARES, L.V.S.; RODRIGUEZ-AMAYA, D.B. Survey of aflatoxins, ochratoxin A, zearalenone and sterigmatocystin in some Brazilian foods by using multi-toxin thin-layer chromatographic method. J
Assoe. Off. Anal. Chem., 72(1):22-26,1989.
22. WATSON, S.A. & RAMSTAD, P.E. 1987 - Com: Chemistry and Technology. St. Paul, M.N.: American Association o/Cereal Chemists, Inc., 605 p.
23. WORLD HEALTH ORGANIZATION - Mycotoxins . Geneva, WHO, 1979. 127p. (EnvironrnentalHealth Criteria, 11).
This work is licensed under a Creative Commons Attribution 4.0 International License.
Copyright (c) 1998 Instituto Adolfo Lutz Journal
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