Keywords
Sickling
Gel-centrifugation
Hemoglobin S
How to Cite
Abstract
The hemoglobin S has been considered a public heath disease due to its high frequency and morbidity. The development of screening methods is important to assist the clinic diagnosis and to help elaborate therapy measures. The peripheral blood of 836 samples were analyzed by the solubility test, the sickling test and the gel-centrifugation test. Twenty-eight samples were positive for hemoglobin S in the three tests analysed, disclosing the same sensibility and specificity for ali of them. However, the solubility test and the gel-centrifugation method were faster than the sickling test, besides requiring short technician training period.
References
2. CHAMBERS, K.; PHILLIPS, A .& CHAMPMAN, C.S. - Use of a low pressure liquid chromatography system for haemoglobinopathy screening. Clin Lab Haematology, 15(2): 119-28, 1993.
3. DACIE, J.V. & LEWIS, S.M. - Practical Haematology 8° ed. Churchill Livingstone, Edinburgh, 1995, p.609.
4. DALAND, G.A. & CASTLE, W.B. - A simple method for demonstration sickling of red blood cells, the use of reducing agents. Ll.ab.Clin.Med., 33:1882,1948.
5. EMMEL, V'E. - A study of the erytrocytes in a cases of severe anemia with elongated sickle shaped red blood corpuscles. Arch. Intern. Med., 20:586,1917.
6. FAIRBANKS, V,E & PETTIT, R.M. - Sickledex test in unstable hemoglobin disorders. I.A. M. A., 220:128, 1972
7. GUSHIKEN, E.Y.; KITAMURA, C.; MIYAMARU- YOKOMIZO,R.; ZAMFIROV,v'M.C.;
ARRUDA, l.C.; MUNHOZ, M.A .G.; CANGERANA-PEREIRA,EA .& MARTINS, H.P. Frequência de hemoglobinopatia S detectada no Instituto Adolfo Lutz-Central e sua importância em saúde pública. Rev. Bras. Anal. Clin., 27(4):l30l32, 1995.
8. HUISMAN, TH The structure and function of normal and abnormal haemoglobins. Clin Haematol., 6(1): 1-30, 1993.
9. ITANO, H.A. Solubilities of naturally occurring mixtures of human hemoglobins. Arch. Biochem. Biophiys,47: 148, 1953.
10. KNAUS, J.S. & HAHN, D.A. - Homozygous alfa thalassemia causing a false negative solubility test in sickle cell trait. Clin. Chem. 27: 1146, 1981.
11. LOREY, E; CUNNINGHAM, G.; SHAFER, E; LUBIN, B.& VICHINSKY, E.- Universal screening for hemoglobinopathies using high-performance liquid chromatography: clinical results of 2.2 milion screens. Eur. I. Hum. Genet., 2(4):262-71, 1994.
12. MONTENI, S.; FRISCHKNECHT, H.& THORMANN, W. Application of dynamic capillary isoelectric focusing to the analysis of human hemoglobin variants. Electrophoresis, 15(1):22-30, 1994.
13. NAOUM, P.C. - Hemoglobinopatias e Talassemias. São Paulo: Editora Sarvier, 1997. p. 171.
14. NAOUM, P.C. - Eletroforese -Técnicas e Diagnósticos. São Paulo: Livraria Santos Editora, 1990. p. 173. capa.
15. VAN DER DIJS, EP; VAN DEN BERG, G.A .; SCHERMER, J.G.; MUSKIET, ED.; LANDMAN, H. & MUSKIET, EA. - Screening cord blood for hemoglobinopathies and thalassemia by HPLC. Clin.Cherm., 38(9):1864-9, 1992.
16. VERRASTRO, T.; LORENZI, T.E & WENDEL NETO, S. - Hematologia e Hemoterapia Fundamentos de morfologia, fisiologia, patologia e clínica. In: VERRASTRO, Therezinha. Principais tipos clínicos de anemia. São Paulo: Atheneu, 1996. 303 p. p. 68-69.
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Copyright (c) 1999 Instituto Adolfo Lutz Journal
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