Keywords
D, L alanine in purple granadilla, determination
passion-fruit
How to Cite
Abstract
Free alanine, a stable natural component of passion-fruit was separated from the aminoacids which were previously extracted from the passion-fruit juice. Circular filter paper chromatography and spectrophotometric determination of the violet deriva te of D, L alanine obtained with nuhydrin were determined. A mean content of 27.0 mg per 10.0 ml of juice was obtained in 25 samples of passion-fruit juice prepared in the laboratory. Values ranging from 4.5 to 30.0 mg per 1'00 ml were found in 32 sarnples of industrial juice. The sensitivity of the method allows detection of free alanine in passion-fruit juices diluted 100 times.
References
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