Keywords
aflatoxins M1 and M2 in cow milk, determination
cow milk, determination of aflatoxin M1 and M2
high-performance liquid chromatography
cow milk, determination of aflatoxin M1 and M2
high-performance liquid chromatography
How to Cite
1.
Sabino M, Purchio A, Zorzetto M Ângela P. Separation and determination of aflatoxins M1 and M2 in samples of cow milk by higk-performance liquid chromatography. Rev Inst Adolfo Lutz [Internet]. 1984 Dec. 28 [cited 2024 Dec. 22];44(2):87-99. Available from: https://periodicos.saude.sp.gov.br/RIAL/article/view/36832
Abstract
Aflatoxins MIo M•• and B, were completely sepa ra ted by high-performance li quid chromatography in reversed-phase C-18 columns by uai ng acetonitrile-water (35% + 65%) as eluting solvent and a flow rate of 2.0 ml/minute. Compounds were detected by ultraviolet absorbance at 350 nm. Peak height and retention time were reproducible. M1 was determined at levels of 0.2 ppb or higher and M2 at 0.1 ppb or higher. Recovery of 87.5% was found for M, and of 102% for M2.
References
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off. anal. Chem" 50 :363-6, 1967.
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17. SEIBER, J.N. & HS1EH, D.P.H. - Application. of high-speed liquid chromato-
graphy to the analysis of aflatoxins. J. Assoc. off. anal. Chem., 56:827-30, 1973.
18, SCHULLER, P.L.; VERHULSDONK, C.A.H. & PAULSCH, W.E. - Analysis of aflatoxin M, in liquid and powdered milk, Pure appl. Chem., 35:291-6, 1973.
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20. STUBBLEF1ELD, R.D. & SHANNON, G.M. - Collaborative study of methods for the determination and chemical confirmation of aflatoxin M, in dairy products, J.
Assoc. off. anal. Chem., 57:852-7, 1974.
21. STUBBLEFIELD, R.D. & SHOTWELL, O.L. - Reverse phase analytical and p reparative h igh pressure Iiquid chromatography of aflatoxins. J. Assoe. off. anal, Chem., 60:784-790, 1977.
22. TAKAHASHI, D.M. & BEEBE, R.M. - Reversed phase h igh performance Iiquid
chromatographic procedure for determiníng aflatoxins in foods and beverag'es using fluorescence detection. In : SYM-POSIUM ON THE ANALYS1S OF FOODS AND BEVERAGES BY HIGH-PRESSURE LIQUID CHROMATOGRAPHY, Honolulu, 1979. Liquid ehromatographie analysis of food and beverap ee, Proceedings, edited by G. Charalambous, New York, Academic press, 1979. v. 1, p. 101.
23, TRUCKSESS, M.W. - Derivative procedure for identification of aflatoxin M, on a thin layer chromatogram. J. Assoc. off. anal. Chem., 59: 722-3, 1976.
24, TRUCKSESS, M.W. & STOLOFF, L. - Extraction, cleanup, and quantitative determination of aflatoxins B, and M, in beef liver. J. Assoc. off. anal. Chem., 62: 1080-2, 1979.
25. TUINSTRA, L.G.M. & BRONSQUEEST, M. - Determination of aflatoxin M, in milk
at the parts per trillion lcvel. J. chrtrmatop r., 111 :448-451, 1975.
26, VAN EGMOND, H.P.; PAULSCH, W.E. & SCHULLER, P.L. - Confirmatory test
for aflatoxin M, on. a thin layer pla te, J. Assoc. off, anal, Chem., 61 :809-12,
1978.
27. WINTERLIN, W.; HALL, G. & HSIEH, D.P.H. On-column chromatographic
extraction of aflatoxin M, from milk and determination by reversed phase high performance liquid chromatography. Anal. Chem., 51: 1873-4, 1979.
28. ZIMMERLI, B. - Verbesserung der Nachweisgrenzo von Aflatoxinen in der Hoch-druck-Flussigkeits-chromatographie durch Verwendung eines kielselfüllten fluorei-zenzdetektors. J. chromatoçr., 131 :458-63, 1977.
analysis of the Association of Official Analytical Chemists. 12nd ed. Washington, D.C., A.C.A.C., 1975. p. 476. [itens 26083-26087]
2. BEEBE, R.M. & TAKAHASHI, D.M. - Determination of aflatoxin M, by high pressure liquid chromatography using fluorescence detection. J. agric. Food
Chem., 28 :481-2, 1980.
3, BROWN, N.L.; NESHEIM, S.; STACK, M.E. & WARE, G.M. - Mcthod for the
determination of aflatoxin in animal tissue. J. Assoc. off, anal; Chem.; 56:
1437-9, 1973.
4. FUKAYAMA, M.; WINTERLIN, W. & HSIEH, D.P.H. - Rapid method for analysis of aflatoxin M, in dairy produts. J. Assoc. off. anal. Chem; 63 :927-30, 1980.
5, GREGORY, J.F. & MANLEY, D. - High performance liquíd chromatographic deter-
mination of aflatoxins in animal tissues and products. J. Assoc. off. anal. Ch.em.,
64: 144-:: 1, 1981.
6, HUNT, D.C.; BOURDON, A.T.; WILD, P.J. & CROSBY, N.T. - Use of high performance Iiquid chromatog raph'y combined wí th fluorescence detection for the iden tification and estimation of aflatoxins and ochratoxin in food. J. Sei, Food Agric., 29: 234-8, 1978.
7, KIERMEIER, F. - Aflatoxin residues in fluid milk. Pure appl. Chem., 35 :271-3, 1973.
8. MAS RI, M.S. - Estimation partition and column chromatographic separation of aflatoxin B, and M,. J. Amer. Oil Chem. Soc., 47:61-4, 1970.
9. MITCHELL, D.J.; SOMERS, E.; CAMPBELL, A.D. & SALWIN, H. - Report of Subcommittee Con Recommendations for Official Methods. J. Assoc. off. anal.
Chem., 57:431, 1974.
10, PANALAKS, T. & SCOTT, P.M. - Sensitive silica gel-packed flowcell for fluorometric detection of afia toxins by high pressure liquid chromatography. J. Assoc. off. anal. Chem., 60: 583-9, 1977.
11, PATTERSON D.S.P.; GLANCY, E.M. & ROBERTS, B.A. - The estimation of aflatoxin M, in milk using a two-dimensional thin-layer chromatographic method suitable for survey work. Food Cosmet. Toxieol., 16 :49-50, 1978.
12, PONS, W.A., Jr. - High pressure liquid chromatographic determination of aflatoxins in corno J. Assoe. off. anal, Chem., 62:586-94, 1979.
13. PONS, W.A., Jr.; CUCULLU, A.F. & LEE, L.S. - Method for the determination of aflatoxin M, in fluid milk and milk products. J. Assoc. off. anal. Chem., 56:
1431-6, 1973.
14, PONS, W.A., Jr. & FRANZ, A.O., Jr. - High performance liquid chromatography of aflatoxins in cottonseed products. J. Assoc. off. anal. Chem., 60:89-95, 1977.
15. PURCHASE, LF.H. & STEYN, M. - Estimation of aflatoxin M, in milk. J. Assoc.
off. anal. Chem" 50 :363-6, 1967.
16, ROBERTS, B.A. & ALLCROFT, R. - A note on the semi-quantitative estimation of afia toxin. M, i n liquid milk by thin -layer chromatography, Food Cosmet. Toxicol., 6: 339, 1968.
17. SEIBER, J.N. & HS1EH, D.P.H. - Application. of high-speed liquid chromato-
graphy to the analysis of aflatoxins. J. Assoc. off. anal. Chem., 56:827-30, 1973.
18, SCHULLER, P.L.; VERHULSDONK, C.A.H. & PAULSCH, W.E. - Analysis of aflatoxin M, in liquid and powdered milk, Pure appl. Chem., 35:291-6, 1973.
19, STUBBLEF1ELD, R.D. - The rapid determination of aflatoxin M, in dairy products. J. Amer. Gil Chem., Soc., 56:800-2, 1979.
20. STUBBLEF1ELD, R.D. & SHANNON, G.M. - Collaborative study of methods for the determination and chemical confirmation of aflatoxin M, in dairy products, J.
Assoc. off. anal. Chem., 57:852-7, 1974.
21. STUBBLEFIELD, R.D. & SHOTWELL, O.L. - Reverse phase analytical and p reparative h igh pressure Iiquid chromatography of aflatoxins. J. Assoe. off. anal, Chem., 60:784-790, 1977.
22. TAKAHASHI, D.M. & BEEBE, R.M. - Reversed phase h igh performance Iiquid
chromatographic procedure for determiníng aflatoxins in foods and beverag'es using fluorescence detection. In : SYM-POSIUM ON THE ANALYS1S OF FOODS AND BEVERAGES BY HIGH-PRESSURE LIQUID CHROMATOGRAPHY, Honolulu, 1979. Liquid ehromatographie analysis of food and beverap ee, Proceedings, edited by G. Charalambous, New York, Academic press, 1979. v. 1, p. 101.
23, TRUCKSESS, M.W. - Derivative procedure for identification of aflatoxin M, on a thin layer chromatogram. J. Assoc. off. anal. Chem., 59: 722-3, 1976.
24, TRUCKSESS, M.W. & STOLOFF, L. - Extraction, cleanup, and quantitative determination of aflatoxins B, and M, in beef liver. J. Assoc. off. anal. Chem., 62: 1080-2, 1979.
25. TUINSTRA, L.G.M. & BRONSQUEEST, M. - Determination of aflatoxin M, in milk
at the parts per trillion lcvel. J. chrtrmatop r., 111 :448-451, 1975.
26, VAN EGMOND, H.P.; PAULSCH, W.E. & SCHULLER, P.L. - Confirmatory test
for aflatoxin M, on. a thin layer pla te, J. Assoc. off, anal, Chem., 61 :809-12,
1978.
27. WINTERLIN, W.; HALL, G. & HSIEH, D.P.H. On-column chromatographic
extraction of aflatoxin M, from milk and determination by reversed phase high performance liquid chromatography. Anal. Chem., 51: 1873-4, 1979.
28. ZIMMERLI, B. - Verbesserung der Nachweisgrenzo von Aflatoxinen in der Hoch-druck-Flussigkeits-chromatographie durch Verwendung eines kielselfüllten fluorei-zenzdetektors. J. chromatoçr., 131 :458-63, 1977.
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Copyright (c) 1984 Instituto Adolfo Lutz Journal
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