Keywords
aflatoxins B1 B2, G1 and G2 determination
peanut, peanut fiour, determination of aflatoxins
high-performance liquid chromatography, use for determining aflatoxins
peanut, peanut fiour, determination of aflatoxins
high-performance liquid chromatography, use for determining aflatoxins
How to Cite
1.
Sabino M, Zorzetto MAP. A method for separation and determination of aflatoxins B1, B2, G1 and G2 by high-performance liquid chromatography. Rev Inst Adolfo Lutz [Internet]. 1984 Dec. 28 [cited 2024 Dec. 21];44(2):101-8. Available from: https://periodicos.saude.sp.gov.br/RIAL/article/view/36833
Abstract
A method for determining aflatoxins B1 B2, G1 and G2 through high-perforrnance liquid chromatography is described. The aflatoxins we re extracted and passed through a reverse-phase chromatography with detection in the ultraviolet spectrum at 350 nm. Recoveries of 92 to 106% were obtained. The method was employed in ten samples of peanut flour. The detection limit was 2 ppb (jLg/kg).
References
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detection of aflatoxins by high-pressure liquid chromatography. J. Assoc. off. anal.
Chem., 60:583-9, 1977.
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9. RAO, G.H.R. & ANDES, M.W. - Aflatoxin detection by high-speed liquid chromato-
graphy and mass spect.rorne try. J. Chromatoqr., 84 :402-6, 1973.
10. SEIBER, J.N. & HSIEH, D.P.H. - Application of high-speed liquid chromatography to the analysis of aflatoxins. J. Assoc. off. anal. Chem., 56:827-30. 1973.
11. SEITZ, L.M. - Comparison of methods for aflatoxin analysis by high-pressure liquid chromatography. J. Chromatogr., 104:81-9, 1975.
12. TAKAHASHI, D.M. - High pressure liquid chromatographic determination of aflatoxins in wines and other Iiquid products. J. Assoc. off. anal. Chem., 60:799-804, 1977.
2. HURST, W.J. & TOOMEY, P.B. - Determination of aflatoxins in peanut products usi ng reverse phase HPLC. J. Chromatogr. Sec., 16: 372-6, 1978.
3. NESHEIM, S. - Methods of aflatoxin anaIysis. In: MATERIALS RESEARCH SYMPOSIUM, 9'", Gaithersburg, Md., 1978. Trace organic analysis: a ncw frontier in analytieal chemistry. Proceedings, edited by H.S. Hertz & S.N. Shesler, Washington, D.C., National Bureau of Standards, 1979. p. 355-72. (NBS special pub. 519)
4. PANALAKS, T. & SCOTT, P.M. - Sensitive silica gel-packed flowcell for f1uorometric
detection of aflatoxins by high-pressure liquid chromatography. J. Assoc. off. anal.
Chem., 60:583-9, 1977.
5. PONS, W.A., Jr. - Resolution of aflatoxins B1 B2 G1 and G2 by high-pressure liquid chromatography. J. Assoc. off. anal. Chem., 59: 101-5, 1976.
6. PONS, W.A., Jr. & FRANZ, A.O., Jr. High performance liquid chromatography of afIa toxins in cottonseed products. J. Assoc. off. anal. Chem., 60: 89-95, 1977.
7. PONS, W.A., Jr. & FRANZ, A.O., Jr. --High pressure-liquid chromatographic determination of aflatoxins in peanut products. J. Assoe. off. anal. Chem., 61: 793-800, 1978.
8. PREGNOLATTO, W. & SABINO, M. Pesquisa e dosagem de aflatoxina em amendoim e derivados e em outros cereais. Rev. Inst. Adolfo Lutz, 29/30:65-71, 1970.
9. RAO, G.H.R. & ANDES, M.W. - Aflatoxin detection by high-speed liquid chromato-
graphy and mass spect.rorne try. J. Chromatoqr., 84 :402-6, 1973.
10. SEIBER, J.N. & HSIEH, D.P.H. - Application of high-speed liquid chromatography to the analysis of aflatoxins. J. Assoc. off. anal. Chem., 56:827-30. 1973.
11. SEITZ, L.M. - Comparison of methods for aflatoxin analysis by high-pressure liquid chromatography. J. Chromatogr., 104:81-9, 1975.
12. TAKAHASHI, D.M. - High pressure liquid chromatographic determination of aflatoxins in wines and other Iiquid products. J. Assoc. off. anal. Chem., 60:799-804, 1977.
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Copyright (c) 1984 Instituto Adolfo Lutz Journal
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