Keywords
staining, immunocitochemical technique for hepatic tissue
antigens, surface, hepatitis B
hepatitis B surface antigens
Australia antigens
antigens, surface, hepatitis B
hepatitis B surface antigens
Australia antigens
How to Cite
1.
Alves VAF, Morais CF de, Santos RT de M, Takeda AK, Gayotto LC da C. Peroxidase - antiperoxidase unlabelled antibody technique (PAP) for the detection of hepatitis B surface antigen in hepatic tissue. Rev Inst Adolfo Lutz [Internet]. 1980 Dec. 30 [cited 2024 May 15];40(2):101-6. Available from: https://periodicos.saude.sp.gov.br/RIAL/article/view/36976
Abstract
Peroxidase - antiperoxidase unlabelled antibody technique on
hepatic tissue sections was carried out for detection of hepatitis B surface antigen. This method demonstrated advantages over immunofluorescence, regardíng to its utilization in fixed 01' in block tissue, in routíne procedures, in optical or electronic microscopy, for visuaJization of detailed morphological tissue structures by selective staining and preparation of permanent records. This technique using ali of the immunological materiaIs prepared in our laboratory at Instituto Adolfo Lutz, São Paulo, Brazil, is an impor tan t step for its suitabiJity for use in diagnostic procedures.
References
1. BURNS, J. - Background staining and sensitivity of the unlabel an tíbody-enzyme PAP method. Comparison with the peroxídase-labelled antibody sandwich method using formalin-fixed paraffin embedded material. Histochemistry, 43: 291-4, 1975.
2. CLAUSEN, P.P.; JACOBSEN, M.; JOHANSEN, P. & THOMMESEN, N. - Immu nohistachemical demonstratian ai in tracelullar immunoglobulin i n formalin iixed, parafiin embedded sections, as staining method in diagnostic work. Acta path. microbiol. scand. (Section C), 87:307-12, 1979.
3. CLAUSEN, P.P. & TOMSEN P. - Dernonstration oi hepatitis B-surface antigen in liver biopsies. Acta path. microbiol. scarul, (Section A), 86 :383-8, 1978.
4. COONS, A.H.; CREECH, H.J. & JONES, R.N. - Immunological properties of an antibody containing a fluorescent group. Proc, Soe. exp. Biol. Med., 47:200-2, 1941.
5. DE LELLIS, R.A.; STERNBERGER, L.A.; MANN, R.B.; BANKS, P.K. & NAKANE, P.K. - Immunoperoxidase technics in diagnostic pathology. Am. J. clin. Path.; 71: 483-8, 1979.
6. HUANG, S.N. - Immunohistochemical demonstration of hepatitis B core and surface antigens in paraifin sections. Lab, lnvest., 33: 88-95, 1975.
7. MESA-TEJADA, R.; PASCAL, R.R. & FENOGLIO, C.M. - Immunoperoxidase: a sensitive immunohistochemical technique as a "special stain" in the diagnostic pathology labo ra to ry. Human. Pathol., 8 :313-20, 1977.
8. MORIARTY, G.C.; MORIARTY, C.M. & STERNBERGER, L.A. - UltrastructuraJ immunocytochemistry with u n labeled an tibodies and the peroxidase-antiperoxidase complex (PAP). A technique more sensitive than radioimmunoassay. J. Histochem. Cytochem., 21: 825-33, 1973.
9. MURPHY, W.M.; DEODHAR, S.D. & CAWLEY, L.P. - Use of horseradish peroxidase in identification of serum antibodies and immune complexes, Clin. Chem., 19 :370, 1973.
10. NAKANE, P.K. & PIERCE, G.B., JR. Enzyme labelled antibodies: preparation and application for the Iocalization oi antigens. J. Histochem. Citochem., 14 :929-38, 1967.
11. RITTER, H.B. & OLESON, J.J. - A pe roxidase reaction in paraffin sections. Arch. Path., 43 :330-2, 1947.
12. SARNO, E.N.; GERECHT, D. & FONSECA, F.B. Unlabelled antibody enzyme method for HBsAg. in liver tissue: soIuble horseradish peroxidase-an tihorseradish peroxidase complex (PAP). Rev. Bras. Med., 35 :488-90, 1978.
13. SHIKATA, T.; UZAWA, T.; YOSHIWARA, N.; AKATSUKA, T. & YAMAZAKI, S. - Staining method oi Australia antigen in paraffin sectian-detection of cytoplasmie inclusion bodies. Jap. J. exp. Med., 44:25-36, 1974.
14. STERNBERGER, L. - Immunochemistry. 2nd ed. New Jersey, Prentice Hall, 1974.
15. STERNBERGER, L.A.; HARDY, P.H., Jr.; CUCULIS, J.J. & MEYER, H.G. - The unlabeled antibody-enzyme method oi immunohistochemistry. Preparation and properties oi soluble antigen-antibody complex (Horseradish peroxidase-antihorse-radish peroxidase) and its use in identification oi spirochetes. J. Histochem. Cytochem., 18 :315-33, 1970.
16. TAYLOR, C.R. - Immunoperoxidase techniques. Practical and theorical aspec ts, Anh. rath. ua, M ed., 102: 113-21, 1978.
17. THUNG, S.N.; GERBER, M.A.; SARNO, E. & POPPER, H. - Distribution of tive antigens in hepatocellular carcinoma. Lab, lnvest., 41: 101, 1979.
2. CLAUSEN, P.P.; JACOBSEN, M.; JOHANSEN, P. & THOMMESEN, N. - Immu nohistachemical demonstratian ai in tracelullar immunoglobulin i n formalin iixed, parafiin embedded sections, as staining method in diagnostic work. Acta path. microbiol. scand. (Section C), 87:307-12, 1979.
3. CLAUSEN, P.P. & TOMSEN P. - Dernonstration oi hepatitis B-surface antigen in liver biopsies. Acta path. microbiol. scarul, (Section A), 86 :383-8, 1978.
4. COONS, A.H.; CREECH, H.J. & JONES, R.N. - Immunological properties of an antibody containing a fluorescent group. Proc, Soe. exp. Biol. Med., 47:200-2, 1941.
5. DE LELLIS, R.A.; STERNBERGER, L.A.; MANN, R.B.; BANKS, P.K. & NAKANE, P.K. - Immunoperoxidase technics in diagnostic pathology. Am. J. clin. Path.; 71: 483-8, 1979.
6. HUANG, S.N. - Immunohistochemical demonstration of hepatitis B core and surface antigens in paraifin sections. Lab, lnvest., 33: 88-95, 1975.
7. MESA-TEJADA, R.; PASCAL, R.R. & FENOGLIO, C.M. - Immunoperoxidase: a sensitive immunohistochemical technique as a "special stain" in the diagnostic pathology labo ra to ry. Human. Pathol., 8 :313-20, 1977.
8. MORIARTY, G.C.; MORIARTY, C.M. & STERNBERGER, L.A. - UltrastructuraJ immunocytochemistry with u n labeled an tibodies and the peroxidase-antiperoxidase complex (PAP). A technique more sensitive than radioimmunoassay. J. Histochem. Cytochem., 21: 825-33, 1973.
9. MURPHY, W.M.; DEODHAR, S.D. & CAWLEY, L.P. - Use of horseradish peroxidase in identification of serum antibodies and immune complexes, Clin. Chem., 19 :370, 1973.
10. NAKANE, P.K. & PIERCE, G.B., JR. Enzyme labelled antibodies: preparation and application for the Iocalization oi antigens. J. Histochem. Citochem., 14 :929-38, 1967.
11. RITTER, H.B. & OLESON, J.J. - A pe roxidase reaction in paraffin sections. Arch. Path., 43 :330-2, 1947.
12. SARNO, E.N.; GERECHT, D. & FONSECA, F.B. Unlabelled antibody enzyme method for HBsAg. in liver tissue: soIuble horseradish peroxidase-an tihorseradish peroxidase complex (PAP). Rev. Bras. Med., 35 :488-90, 1978.
13. SHIKATA, T.; UZAWA, T.; YOSHIWARA, N.; AKATSUKA, T. & YAMAZAKI, S. - Staining method oi Australia antigen in paraffin sectian-detection of cytoplasmie inclusion bodies. Jap. J. exp. Med., 44:25-36, 1974.
14. STERNBERGER, L. - Immunochemistry. 2nd ed. New Jersey, Prentice Hall, 1974.
15. STERNBERGER, L.A.; HARDY, P.H., Jr.; CUCULIS, J.J. & MEYER, H.G. - The unlabeled antibody-enzyme method oi immunohistochemistry. Preparation and properties oi soluble antigen-antibody complex (Horseradish peroxidase-antihorse-radish peroxidase) and its use in identification oi spirochetes. J. Histochem. Cytochem., 18 :315-33, 1970.
16. TAYLOR, C.R. - Immunoperoxidase techniques. Practical and theorical aspec ts, Anh. rath. ua, M ed., 102: 113-21, 1978.
17. THUNG, S.N.; GERBER, M.A.; SARNO, E. & POPPER, H. - Distribution of tive antigens in hepatocellular carcinoma. Lab, lnvest., 41: 101, 1979.
This work is licensed under a Creative Commons Attribution 4.0 International License.
Copyright (c) 1980 Instituto Adolfo Lutz Journal
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