Palabras clave
coloração de tecido hepático, técnica imunocitoquímica
antígenos de superfície da hepatite B
hepatite B, antígenos
antígeno Austrália
antígenos de superfície da hepatite B
hepatite B, antígenos
antígeno Austrália
Cómo citar
1.
Alves VAF, Morais CF de, Santos RT de M, Takeda AK, Gayotto LC da C. Utilização da técnica de anticorpos não marcados peroxidase (PAP) na detecção do antígeno de superfície da hepatite B em tecido hepático. Rev Inst Adolfo Lutz [Internet]. 30 de diciembre de 1980 [citado 10 de enero de 2025];40(2):101-6. Disponible en: https://periodicos.saude.sp.gov.br/RIAL/article/view/36976
Resumen
Foi utilizada a técnica de anticorpos não marcados peroxidase-antiperoxidase em fragmentos de tecido hepático para a detecção de antígeno de superfície da hepatite B. Em confronto com a imunofluorescência, esta reação apresenta as vantagens de aplicabilidade em tecidos fixados e emblocados rotineiramente, análise à microscopia óptica ou eletrônica, visualização de detalhes morfológicos do tecido por contracorantes, além de manutenção da reação por um tempo indefenido. A utilização, neste estudo, de arsenal imunológico totalmente produzido nos laboratórios do Instituto Adolfo Lutz, São Paulo, Brasil, é um importante pa ss o para a aplicabilidade deste método na rotina diagnóstica, em nosso meio.
Citas
1. BURNS, J. - Background staining and sensitivity of the unlabel an tíbody-enzyme PAP method. Comparison with the peroxídase-labelled antibody sandwich method using formalin-fixed paraffin embedded material. Histochemistry, 43: 291-4, 1975.
2. CLAUSEN, P.P.; JACOBSEN, M.; JOHANSEN, P. & THOMMESEN, N. - Immu nohistachemical demonstratian ai in tracelullar immunoglobulin i n formalin iixed, parafiin embedded sections, as staining method in diagnostic work. Acta path. microbiol. scand. (Section C), 87:307-12, 1979.
3. CLAUSEN, P.P. & TOMSEN P. - Dernonstration oi hepatitis B-surface antigen in liver biopsies. Acta path. microbiol. scarul, (Section A), 86 :383-8, 1978.
4. COONS, A.H.; CREECH, H.J. & JONES, R.N. - Immunological properties of an antibody containing a fluorescent group. Proc, Soe. exp. Biol. Med., 47:200-2, 1941.
5. DE LELLIS, R.A.; STERNBERGER, L.A.; MANN, R.B.; BANKS, P.K. & NAKANE, P.K. - Immunoperoxidase technics in diagnostic pathology. Am. J. clin. Path.; 71: 483-8, 1979.
6. HUANG, S.N. - Immunohistochemical demonstration of hepatitis B core and surface antigens in paraifin sections. Lab, lnvest., 33: 88-95, 1975.
7. MESA-TEJADA, R.; PASCAL, R.R. & FENOGLIO, C.M. - Immunoperoxidase: a sensitive immunohistochemical technique as a "special stain" in the diagnostic pathology labo ra to ry. Human. Pathol., 8 :313-20, 1977.
8. MORIARTY, G.C.; MORIARTY, C.M. & STERNBERGER, L.A. - UltrastructuraJ immunocytochemistry with u n labeled an tibodies and the peroxidase-antiperoxidase complex (PAP). A technique more sensitive than radioimmunoassay. J. Histochem. Cytochem., 21: 825-33, 1973.
9. MURPHY, W.M.; DEODHAR, S.D. & CAWLEY, L.P. - Use of horseradish peroxidase in identification of serum antibodies and immune complexes, Clin. Chem., 19 :370, 1973.
10. NAKANE, P.K. & PIERCE, G.B., JR. Enzyme labelled antibodies: preparation and application for the Iocalization oi antigens. J. Histochem. Citochem., 14 :929-38, 1967.
11. RITTER, H.B. & OLESON, J.J. - A pe roxidase reaction in paraffin sections. Arch. Path., 43 :330-2, 1947.
12. SARNO, E.N.; GERECHT, D. & FONSECA, F.B. Unlabelled antibody enzyme method for HBsAg. in liver tissue: soIuble horseradish peroxidase-an tihorseradish peroxidase complex (PAP). Rev. Bras. Med., 35 :488-90, 1978.
13. SHIKATA, T.; UZAWA, T.; YOSHIWARA, N.; AKATSUKA, T. & YAMAZAKI, S. - Staining method oi Australia antigen in paraffin sectian-detection of cytoplasmie inclusion bodies. Jap. J. exp. Med., 44:25-36, 1974.
14. STERNBERGER, L. - Immunochemistry. 2nd ed. New Jersey, Prentice Hall, 1974.
15. STERNBERGER, L.A.; HARDY, P.H., Jr.; CUCULIS, J.J. & MEYER, H.G. - The unlabeled antibody-enzyme method oi immunohistochemistry. Preparation and properties oi soluble antigen-antibody complex (Horseradish peroxidase-antihorse-radish peroxidase) and its use in identification oi spirochetes. J. Histochem. Cytochem., 18 :315-33, 1970.
16. TAYLOR, C.R. - Immunoperoxidase techniques. Practical and theorical aspec ts, Anh. rath. ua, M ed., 102: 113-21, 1978.
17. THUNG, S.N.; GERBER, M.A.; SARNO, E. & POPPER, H. - Distribution of tive antigens in hepatocellular carcinoma. Lab, lnvest., 41: 101, 1979.
2. CLAUSEN, P.P.; JACOBSEN, M.; JOHANSEN, P. & THOMMESEN, N. - Immu nohistachemical demonstratian ai in tracelullar immunoglobulin i n formalin iixed, parafiin embedded sections, as staining method in diagnostic work. Acta path. microbiol. scand. (Section C), 87:307-12, 1979.
3. CLAUSEN, P.P. & TOMSEN P. - Dernonstration oi hepatitis B-surface antigen in liver biopsies. Acta path. microbiol. scarul, (Section A), 86 :383-8, 1978.
4. COONS, A.H.; CREECH, H.J. & JONES, R.N. - Immunological properties of an antibody containing a fluorescent group. Proc, Soe. exp. Biol. Med., 47:200-2, 1941.
5. DE LELLIS, R.A.; STERNBERGER, L.A.; MANN, R.B.; BANKS, P.K. & NAKANE, P.K. - Immunoperoxidase technics in diagnostic pathology. Am. J. clin. Path.; 71: 483-8, 1979.
6. HUANG, S.N. - Immunohistochemical demonstration of hepatitis B core and surface antigens in paraifin sections. Lab, lnvest., 33: 88-95, 1975.
7. MESA-TEJADA, R.; PASCAL, R.R. & FENOGLIO, C.M. - Immunoperoxidase: a sensitive immunohistochemical technique as a "special stain" in the diagnostic pathology labo ra to ry. Human. Pathol., 8 :313-20, 1977.
8. MORIARTY, G.C.; MORIARTY, C.M. & STERNBERGER, L.A. - UltrastructuraJ immunocytochemistry with u n labeled an tibodies and the peroxidase-antiperoxidase complex (PAP). A technique more sensitive than radioimmunoassay. J. Histochem. Cytochem., 21: 825-33, 1973.
9. MURPHY, W.M.; DEODHAR, S.D. & CAWLEY, L.P. - Use of horseradish peroxidase in identification of serum antibodies and immune complexes, Clin. Chem., 19 :370, 1973.
10. NAKANE, P.K. & PIERCE, G.B., JR. Enzyme labelled antibodies: preparation and application for the Iocalization oi antigens. J. Histochem. Citochem., 14 :929-38, 1967.
11. RITTER, H.B. & OLESON, J.J. - A pe roxidase reaction in paraffin sections. Arch. Path., 43 :330-2, 1947.
12. SARNO, E.N.; GERECHT, D. & FONSECA, F.B. Unlabelled antibody enzyme method for HBsAg. in liver tissue: soIuble horseradish peroxidase-an tihorseradish peroxidase complex (PAP). Rev. Bras. Med., 35 :488-90, 1978.
13. SHIKATA, T.; UZAWA, T.; YOSHIWARA, N.; AKATSUKA, T. & YAMAZAKI, S. - Staining method oi Australia antigen in paraffin sectian-detection of cytoplasmie inclusion bodies. Jap. J. exp. Med., 44:25-36, 1974.
14. STERNBERGER, L. - Immunochemistry. 2nd ed. New Jersey, Prentice Hall, 1974.
15. STERNBERGER, L.A.; HARDY, P.H., Jr.; CUCULIS, J.J. & MEYER, H.G. - The unlabeled antibody-enzyme method oi immunohistochemistry. Preparation and properties oi soluble antigen-antibody complex (Horseradish peroxidase-antihorse-radish peroxidase) and its use in identification oi spirochetes. J. Histochem. Cytochem., 18 :315-33, 1970.
16. TAYLOR, C.R. - Immunoperoxidase techniques. Practical and theorical aspec ts, Anh. rath. ua, M ed., 102: 113-21, 1978.
17. THUNG, S.N.; GERBER, M.A.; SARNO, E. & POPPER, H. - Distribution of tive antigens in hepatocellular carcinoma. Lab, lnvest., 41: 101, 1979.
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Derechos de autor 1980 Revista del Instituto Adolfo Lutz
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