Palabras clave
deoxinivalenol
análise milho
trigo
cromatografia em camada delgada
análise milho
trigo
cromatografia em camada delgada
Cómo citar
1.
Sabino M, Ichikawa AH, Inomata EI, Lamardo LCA. Determinação de deoxinivalenol em trigo e milho em grão por cromatografia em camada delgada. Rev Inst Adolfo Lutz [Internet]. 29 de diciembre de 1989 [citado 25 de noviembre de 2024];49(2):155-9. Disponible en: https://periodicos.saude.sp.gov.br/RIAL/article/view/36946
Resumen
Em amostras de milho em grão e trigo de várias procedências, foi verificada a ocorrência de deoxinivalenol (DON), utilizando a técnica de cromatografia em camada delgada. Das 120 amostras analisadas, apenas duas apresentaram deoxinivalenol com concentração de 183,0 ).lg!kg . O limite de determinação do método foi de 110,0 ug on meg. Os testes de recuperação variaram de 80 a 100% para o trigo e de 75 a 100% para o milho com médias de 90 a 85%, espectivamente. O coeficiente de variação foi de 11 %, tanto para o trigo como para o milho.
Citas
1. CHANG, H.L.; DEVRIES, J.W.; LARSON, P.A. & PATEL, H.H. - Rapid determination of deoxynivalenol (vomitoxin) by liquid chromatography using modified Romer column cleanup. l.Assoc. off. anal . Chem. 67:52-4, 1984.
2. CÔTÉ,1.M.; NICOLLETTI, J.; SW ANSON, S.P. & BUCK, W.B. - Production of deepoxy deoxynivalenol (DOM-I), a metabolite of deoxynivalenol, by in vitro rumen incubation. J, agric. food Chem; 34:458-60, 1986.
3. EPPLEY, R.M.; TRUCKSESS, M.W.; NESHEIM, S.; THORPE, C.W.; WOOD, G.E. & POHLAND, A.E. - Deoxynivalenol winter wheat: thin layer chromatographic method and survey. l. Assoc. off. anal. Chem, 67:43-5, 1984.
4. FONSECA, H. - Contribuição e estudo de afia toxina no amendoim (Araehis hypogaea L.) da colheita à industrialização. Piracicaba 1969. 98p. [Tese livre docência - Escola Superior de Agricultura "Luiz de Queirós" - Universidade de São Paulo].
5. FORSYTH, D.M.; YOSHIZA WA, T.;MOROOKA, N. & TUITE, J. - Emetic and refusal activity of deoxynivalenolto swuine. Appl. environ, Microbiol., 34:547-52, 1977.
6. JOSEFSSON, E. & MOLLER, T.E. - Screening method for the detection of aflatoxins, ochratoxin, patulin, sterigmatocystin, and zearalenone in cereals. J, Assoc. off. anal, Chem., 60:1369-71, 1977.
7. KHERA, K.S.; ARNOLD, D.L.; WHALEN, C.; ANGERS. G. & SCOTT, P.M. - Vomitoxin (4-Deoxynivalenol): Effects on reproduction of mice and rats. Toxicolog. appl. Pharmacol.,74:345-56, 1984.
8. MIROCHA, cr, PATHRE, S. V.; SCHAUERHAMER, B. & CHRISTENSEN, C.M. - Natural occurrence of Fusarium toxins in feedstuff. Appl. Environ,
Microbiol., 32:553-6, 1976.
9. PATHRE, S.V. & MIROCHA, C.I. - Trichothecenes: natural occurrence and potential hazard. J. am. Oil Chem. Soc., 56:820-823, 1979.
10. PATTERSON, D.S.P. & ROBERTS, B.A. - Mycotoxins in animal feedstuffs: sensitive thin layer chromatographic detection of aflatoxin, ochratoxin A, sterigmatocystin, zearalenone, and T-2 toxin. I. Assoc. off. anal. Chem, 62: 1265-67, 1979.
11. PRADO, G. - Incidência de aflatoxina B1 em alimentos. Rev. Farm. Bioquim., 5: 147-57, 1983.
12. ROBERTS, B.A.; GLANCY, E.M. & PATTERSON, D.S.P. - Rapid, economical method for detennination of aflatoxin and ochratoxin in animal feedstuffs. J. Assoc. off. anal . Chem., 64:961 - 3, 1981.
13. SABINO, M. - Variações de níveis de aflatoxina B 1 em alimentos e rações animais no período de 1971 a 1979. Rev. Inst, Adolfo Lutz, 40:153-8, 1980.
14. SABINO, M. & CORRÊA, M.I.S. - Aflatoxina B1 em feijão. Rev. Inst. Adolfo Lutz ; 41:837-7, 1981.
15. SABINO, M.; INOMATA, E.I. & LAMARDO, L.C.A. - Variação dos níveis de aflatoxina B1 em pasta de amendoim e paçoca consumi das no Estado de São Paulo. Rev, Inst. Adolfo Lutz, 42:39-44, 1982.
16. SCOTT. P.M. - Assessment of quantitative methods for determination of trichothecenes in grains and grain products. J. Assoc. off. anal. Chem., 65:876-83, 1982.
17. SCUSSEL, V.M. & RODRIGUEZ-AMAYA, D.B.Teores de aflatoxinas em amendoim e seus produtos comercializados em Campinas em 1980/82. Boi. Soe. bras. Ciênc. Tecnol. Alimen., Campinas, 19:109-19, 1985.
18. SCUSSEL V.M.; RODRIGUEZ -AMAYA, D.B. & SILVA, W.J. - Incidência de aflatoxina em milho (Zea mays L.) e em seus produtos derivados, comercializados na região de Campinas, Estado de São Paulo, Brasil. Ciênc. Tecnol. Aliment. 6:7585, 1986.
19. SOARES, L.M.V. & RODRIGUEZ-AMAYA, D.B.Survey of aflatoxins, Ochratoxin A, Zeralenone, and Sterigmatocystin in Some Brazilian Foods by Using Multi - toxin Thin-Layer Chromat.ographic Method. J. Assoc. off. anal. Chem., 72:22-6, 1989.
20. STOLOFF, L. & SCOTT, P.M. - Natural poisons. In: ASSOCIA TION OF OFFICIAL ANAL YTICAL CHEMISTS - Offieial methods of analysis of the Association of Official Analytical chemists 14 th ed. Arlington, Virginia, A.O.A.C., 1984. p. 479 [item 26009].
21. TANAKA, T.; HASEGAWA, A.; YAMAMOTO, S.; LEE, U.S.; SUGIURA, Y. & UENO Y. - Worldwide contamination of cereals by the Fusarium mycotoxins nivalenol, deoxynivalenol and zearalenone. 1. Survey of 19 countries. J. agric. Food Chem., 36:979-83, 1988.
22. TERHUNE, S.I.; NGUYEN, N.V.; BAXTER, I.A.; PRYDE, D.H. & QURESHI, S.A. - Improved gas chromatographic method for quantitation of deoxynivalenol in wheat, com and feed. J. Assoc. off. anal. Chem., 67:1102-04, 1984.
23. TRENHOLM, H.L.; COCHRANE, W.P.; COHEN, H,; ELLIOT, 1.1.; FARNWORTH, E.R.; FRIEND, D.W.; HAMILTON, R.M.G.; STANDISH, I.F. & THOMPSON, B.K. - Survey of vomitoxin contamination of 1980 Ontario white winter wheat crop: results of survey and feeding trials. J. Assoc. off. anal. Chem., 66:92-7, 1983.
24. TRENHOLM, H.L.; THOMPSON, B.K.; HARTIN, K.E.; GREENHALGH, R. & McALLISTER, A.I. - Ingestion of vomitoxin (deoxynivalenol) contaminated wheat by nonlactaning dairy cows. J. Dairy Sci., 68:1000-5, 1985.
25. TRUCKSESS, M.W.; NESHEIM, S. & EPPLEY, R.M. - Thin-Iayer chromatographic determination of deoxynivalenol in wheat and com. J. Assoc.off. anal. Chem., 67:40-3, 1984.
26. VESONDER, R.F.; CIEGLER, A. & JENSEN, A.H. Isolation of the emetic principie from Fusarium infected com. Appl. Microbiol., 26:1008-10, 1973.
27. VESONDER, R.F.; CIEGLER, A.; JENSEN, A.H.; ROHWEDDER, W.K. & WEISLEDER, D. et alii Co-identity of the refusal and emetic principie from Fusarium infected com. Appl. Environ. Microbiol., 31:280-5, 1976.
28. XU, YI-CHUN; ZHANG, G.S. & CHU, FUN S. Enzyme-Linked Imunosorbent assay for deoxynivalenol in com and wheat. I. Assoc, off. anal. chem., 71(5):945-9, 1988.
29. YOSHIZA WA, T. & MOROOKA, N. - Deoxynivalenol and its monoacetate: new mycotoxins from Fusarium roseum and moldy barley. Agric. biol. Chem., 37:2933-34, 1973.
30. YOSHIZAWA, T.; TAKEDA, H. & OHI, T. - Structure of a novel metabolite from deoxynivalenol, a trichothecene mycotoxin, in animals. Agric. Biol. Chem., 47: 2133-5, 1983.
31. YOUNG, I.C.; FULCIIER, R.G.; HAYHOE, I.H.; SCOTT, P.M. & DEXTER, I.E. - Effect of milling and baking on deoxynivalenol (vomitoxin) content of eastem canadian wheats. J. agric. Food Chem.,32(3): 659-64, 1984.
2. CÔTÉ,1.M.; NICOLLETTI, J.; SW ANSON, S.P. & BUCK, W.B. - Production of deepoxy deoxynivalenol (DOM-I), a metabolite of deoxynivalenol, by in vitro rumen incubation. J, agric. food Chem; 34:458-60, 1986.
3. EPPLEY, R.M.; TRUCKSESS, M.W.; NESHEIM, S.; THORPE, C.W.; WOOD, G.E. & POHLAND, A.E. - Deoxynivalenol winter wheat: thin layer chromatographic method and survey. l. Assoc. off. anal. Chem, 67:43-5, 1984.
4. FONSECA, H. - Contribuição e estudo de afia toxina no amendoim (Araehis hypogaea L.) da colheita à industrialização. Piracicaba 1969. 98p. [Tese livre docência - Escola Superior de Agricultura "Luiz de Queirós" - Universidade de São Paulo].
5. FORSYTH, D.M.; YOSHIZA WA, T.;MOROOKA, N. & TUITE, J. - Emetic and refusal activity of deoxynivalenolto swuine. Appl. environ, Microbiol., 34:547-52, 1977.
6. JOSEFSSON, E. & MOLLER, T.E. - Screening method for the detection of aflatoxins, ochratoxin, patulin, sterigmatocystin, and zearalenone in cereals. J, Assoc. off. anal, Chem., 60:1369-71, 1977.
7. KHERA, K.S.; ARNOLD, D.L.; WHALEN, C.; ANGERS. G. & SCOTT, P.M. - Vomitoxin (4-Deoxynivalenol): Effects on reproduction of mice and rats. Toxicolog. appl. Pharmacol.,74:345-56, 1984.
8. MIROCHA, cr, PATHRE, S. V.; SCHAUERHAMER, B. & CHRISTENSEN, C.M. - Natural occurrence of Fusarium toxins in feedstuff. Appl. Environ,
Microbiol., 32:553-6, 1976.
9. PATHRE, S.V. & MIROCHA, C.I. - Trichothecenes: natural occurrence and potential hazard. J. am. Oil Chem. Soc., 56:820-823, 1979.
10. PATTERSON, D.S.P. & ROBERTS, B.A. - Mycotoxins in animal feedstuffs: sensitive thin layer chromatographic detection of aflatoxin, ochratoxin A, sterigmatocystin, zearalenone, and T-2 toxin. I. Assoc. off. anal. Chem, 62: 1265-67, 1979.
11. PRADO, G. - Incidência de aflatoxina B1 em alimentos. Rev. Farm. Bioquim., 5: 147-57, 1983.
12. ROBERTS, B.A.; GLANCY, E.M. & PATTERSON, D.S.P. - Rapid, economical method for detennination of aflatoxin and ochratoxin in animal feedstuffs. J. Assoc. off. anal . Chem., 64:961 - 3, 1981.
13. SABINO, M. - Variações de níveis de aflatoxina B 1 em alimentos e rações animais no período de 1971 a 1979. Rev. Inst, Adolfo Lutz, 40:153-8, 1980.
14. SABINO, M. & CORRÊA, M.I.S. - Aflatoxina B1 em feijão. Rev. Inst. Adolfo Lutz ; 41:837-7, 1981.
15. SABINO, M.; INOMATA, E.I. & LAMARDO, L.C.A. - Variação dos níveis de aflatoxina B1 em pasta de amendoim e paçoca consumi das no Estado de São Paulo. Rev, Inst. Adolfo Lutz, 42:39-44, 1982.
16. SCOTT. P.M. - Assessment of quantitative methods for determination of trichothecenes in grains and grain products. J. Assoc. off. anal. Chem., 65:876-83, 1982.
17. SCUSSEL, V.M. & RODRIGUEZ-AMAYA, D.B.Teores de aflatoxinas em amendoim e seus produtos comercializados em Campinas em 1980/82. Boi. Soe. bras. Ciênc. Tecnol. Alimen., Campinas, 19:109-19, 1985.
18. SCUSSEL V.M.; RODRIGUEZ -AMAYA, D.B. & SILVA, W.J. - Incidência de aflatoxina em milho (Zea mays L.) e em seus produtos derivados, comercializados na região de Campinas, Estado de São Paulo, Brasil. Ciênc. Tecnol. Aliment. 6:7585, 1986.
19. SOARES, L.M.V. & RODRIGUEZ-AMAYA, D.B.Survey of aflatoxins, Ochratoxin A, Zeralenone, and Sterigmatocystin in Some Brazilian Foods by Using Multi - toxin Thin-Layer Chromat.ographic Method. J. Assoc. off. anal. Chem., 72:22-6, 1989.
20. STOLOFF, L. & SCOTT, P.M. - Natural poisons. In: ASSOCIA TION OF OFFICIAL ANAL YTICAL CHEMISTS - Offieial methods of analysis of the Association of Official Analytical chemists 14 th ed. Arlington, Virginia, A.O.A.C., 1984. p. 479 [item 26009].
21. TANAKA, T.; HASEGAWA, A.; YAMAMOTO, S.; LEE, U.S.; SUGIURA, Y. & UENO Y. - Worldwide contamination of cereals by the Fusarium mycotoxins nivalenol, deoxynivalenol and zearalenone. 1. Survey of 19 countries. J. agric. Food Chem., 36:979-83, 1988.
22. TERHUNE, S.I.; NGUYEN, N.V.; BAXTER, I.A.; PRYDE, D.H. & QURESHI, S.A. - Improved gas chromatographic method for quantitation of deoxynivalenol in wheat, com and feed. J. Assoc. off. anal. Chem., 67:1102-04, 1984.
23. TRENHOLM, H.L.; COCHRANE, W.P.; COHEN, H,; ELLIOT, 1.1.; FARNWORTH, E.R.; FRIEND, D.W.; HAMILTON, R.M.G.; STANDISH, I.F. & THOMPSON, B.K. - Survey of vomitoxin contamination of 1980 Ontario white winter wheat crop: results of survey and feeding trials. J. Assoc. off. anal. Chem., 66:92-7, 1983.
24. TRENHOLM, H.L.; THOMPSON, B.K.; HARTIN, K.E.; GREENHALGH, R. & McALLISTER, A.I. - Ingestion of vomitoxin (deoxynivalenol) contaminated wheat by nonlactaning dairy cows. J. Dairy Sci., 68:1000-5, 1985.
25. TRUCKSESS, M.W.; NESHEIM, S. & EPPLEY, R.M. - Thin-Iayer chromatographic determination of deoxynivalenol in wheat and com. J. Assoc.off. anal. Chem., 67:40-3, 1984.
26. VESONDER, R.F.; CIEGLER, A. & JENSEN, A.H. Isolation of the emetic principie from Fusarium infected com. Appl. Microbiol., 26:1008-10, 1973.
27. VESONDER, R.F.; CIEGLER, A.; JENSEN, A.H.; ROHWEDDER, W.K. & WEISLEDER, D. et alii Co-identity of the refusal and emetic principie from Fusarium infected com. Appl. Environ. Microbiol., 31:280-5, 1976.
28. XU, YI-CHUN; ZHANG, G.S. & CHU, FUN S. Enzyme-Linked Imunosorbent assay for deoxynivalenol in com and wheat. I. Assoc, off. anal. chem., 71(5):945-9, 1988.
29. YOSHIZA WA, T. & MOROOKA, N. - Deoxynivalenol and its monoacetate: new mycotoxins from Fusarium roseum and moldy barley. Agric. biol. Chem., 37:2933-34, 1973.
30. YOSHIZAWA, T.; TAKEDA, H. & OHI, T. - Structure of a novel metabolite from deoxynivalenol, a trichothecene mycotoxin, in animals. Agric. Biol. Chem., 47: 2133-5, 1983.
31. YOUNG, I.C.; FULCIIER, R.G.; HAYHOE, I.H.; SCOTT, P.M. & DEXTER, I.E. - Effect of milling and baking on deoxynivalenol (vomitoxin) content of eastem canadian wheats. J. agric. Food Chem.,32(3): 659-64, 1984.
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Derechos de autor 1989 Revista del Instituto Adolfo Lutz
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