Microscopia de fluorescência como procedimento para a realização do exame baciloscôpico em hanseníase
DOI:
https://doi.org/10.47878/hi.1991.v16.35228Palavras-chave:
Mycobacterium leprae, Microscopia, ColoraçõesResumo
Visando comparar os métodos de microscope fluorescente e de Ziehl-Neelsen para evidenciaçâo de bacilos resistentes em material proveniente de derme de pacientes hansenlanos, foram examinados 355 esfregaços. Obteve-se 98,81% de concordância ajustada (KAPPA)entre os métodos. Verificou-se no entanto aproximadamente 55% de resuftados discordantes em relação ao índice baciloscdpico (Ridley), decorrentes de contagens de bacilos por campo mais elevadas pelo método fluorescente.
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Referências
1 BRASIL Ministério da Saúde. Secretaria Nacional de Programas Especiais de Saúde. DMsão Nacional de Dermatologia Sanitária. Normas técnicas e procedimentos para o exame baciloscópio em hanseníase. Brasilia, 1989, 32p.
2 COLLINS, N.M.; MORRISON, N.E.; DHOPLE, A.M.; WATSON, S.R. Microscopic counts carried out on Mycobacterium leprae and
Mycobacterium tuberculosis suspensions. A comparison of three stain- ing procedures. Int. J. Lepr., 48(4):402-407,1980.
3 COSTA, H.C.; SOUZA, LCD.; MARTINI, J.P.; FERRAZOLI, L MARTINS, M.O.; OPROMOLLA, D.V.A.; DEL GIUDICE, A. C. Estudo comparativo das variantes do método de coloração de micobactórias. Hansen. Int., 13(2):37- 41, 1988.
4 FARIA, J.L. Fluorescent staining for Mycobacterium leprae in tissue sec- tions comparison with Fite- Faraco Procedure. Int. J. Lepr., 42:52-54, 1974.
5 DUBOIS, A. & SWERTZ, L L'emploi du micro- scope a fluorescence dans le diagnostic de la lepre. Ann. Soc. Belg. Med. Trop., 30:1473, 1950 (also abstracted In Int. J. Lepr., 19:260, 1951).
6 HAGEMANN, P.K.H. Fluorescence of Mycobacteria with auramine. Muchen Med. Wschr., 85:1066, 1938.
7 HANKS, J.H.; CHATTERGEE, M.B.; LECHAT, M.F. A guide to the couting ofmycobacteria in clinical and experimental materials. Int. J. Lepr., 32(2): 156- 167, 1964.
8 HARDAS, V. & LELE, V. Evaluation of fluorescent microscopy for detection of Mycobacterium leprae. Lepr. India, 53(2):273-277, 1981.
9 JARIWALA, H.J. & KELKAR, S.S. Fluores- cence microscopy for detection of M. leprae in tissue sections. Int. J. Lepr., 47(1):33-36, 1979.
10 KUPER, S.W.A. & MAY, JR. Detection of acid-fast organisms in tissue sections by fluorescence microscopy. J. Path. Beet., 79:59-68, 1960.
11 LEIKER, D.L & McDOUGALL, A.C. Technical guide for smear examination for leprosy by direct microscopy. Infolep-Royal Tropical
institute. Amsterdam, p.35.
12 MANSFIELD, R.E. An improved method for the fluorochrome staining of mycobacteria in tissues and smears. Am. J. Olin. Pathol.,
53:394-406, 1970.
13 PRENDES, M.A.G.; CARBONELE, A.F.; PARDOCASTELL0, V.; HERNANDES, A.C. La microscopia fluorescente em le prolog la .
Valoracion del metodo fluorescente frente al Ziehl-Neelsen en el diagnóstico bacteriológico. Int. J. Lepr., 21(1):35- 40, 1953.
14 RICHARDS, O.W. & MILLER, D.K. An efficient method for the identification of tuberculosis bacteria with a simple fluorescent microscope. Am. J. Win. Pathol. Tech. Suppl., 5:1-8, 1941.
15 RIDLEY, D.S. Bacterial indices. In:COCHRANE,R.G. &DAVEY, T. Leprosy in theory and practice, 2nd ed. Bristol: John Wright & Sons, 1964, 659p.
16 SHEPARD, C.C. & McRAE, D.H. A method for counting acid fast bacteria. Int. J. Lepr., 36:78-82, 1968.
17 UNDP/WORLD BANK/WHO. Special pro- gramme for Research and Training in Tropical Diseases. IMMLEP. Recom- mended safety requirements for the preparation of lepromin: a WHO memorandum. Bulletin of World Health Organization, 57(6):921- 923, 1979.
2 COLLINS, N.M.; MORRISON, N.E.; DHOPLE, A.M.; WATSON, S.R. Microscopic counts carried out on Mycobacterium leprae and
Mycobacterium tuberculosis suspensions. A comparison of three stain- ing procedures. Int. J. Lepr., 48(4):402-407,1980.
3 COSTA, H.C.; SOUZA, LCD.; MARTINI, J.P.; FERRAZOLI, L MARTINS, M.O.; OPROMOLLA, D.V.A.; DEL GIUDICE, A. C. Estudo comparativo das variantes do método de coloração de micobactórias. Hansen. Int., 13(2):37- 41, 1988.
4 FARIA, J.L. Fluorescent staining for Mycobacterium leprae in tissue sec- tions comparison with Fite- Faraco Procedure. Int. J. Lepr., 42:52-54, 1974.
5 DUBOIS, A. & SWERTZ, L L'emploi du micro- scope a fluorescence dans le diagnostic de la lepre. Ann. Soc. Belg. Med. Trop., 30:1473, 1950 (also abstracted In Int. J. Lepr., 19:260, 1951).
6 HAGEMANN, P.K.H. Fluorescence of Mycobacteria with auramine. Muchen Med. Wschr., 85:1066, 1938.
7 HANKS, J.H.; CHATTERGEE, M.B.; LECHAT, M.F. A guide to the couting ofmycobacteria in clinical and experimental materials. Int. J. Lepr., 32(2): 156- 167, 1964.
8 HARDAS, V. & LELE, V. Evaluation of fluorescent microscopy for detection of Mycobacterium leprae. Lepr. India, 53(2):273-277, 1981.
9 JARIWALA, H.J. & KELKAR, S.S. Fluores- cence microscopy for detection of M. leprae in tissue sections. Int. J. Lepr., 47(1):33-36, 1979.
10 KUPER, S.W.A. & MAY, JR. Detection of acid-fast organisms in tissue sections by fluorescence microscopy. J. Path. Beet., 79:59-68, 1960.
11 LEIKER, D.L & McDOUGALL, A.C. Technical guide for smear examination for leprosy by direct microscopy. Infolep-Royal Tropical
institute. Amsterdam, p.35.
12 MANSFIELD, R.E. An improved method for the fluorochrome staining of mycobacteria in tissues and smears. Am. J. Olin. Pathol.,
53:394-406, 1970.
13 PRENDES, M.A.G.; CARBONELE, A.F.; PARDOCASTELL0, V.; HERNANDES, A.C. La microscopia fluorescente em le prolog la .
Valoracion del metodo fluorescente frente al Ziehl-Neelsen en el diagnóstico bacteriológico. Int. J. Lepr., 21(1):35- 40, 1953.
14 RICHARDS, O.W. & MILLER, D.K. An efficient method for the identification of tuberculosis bacteria with a simple fluorescent microscope. Am. J. Win. Pathol. Tech. Suppl., 5:1-8, 1941.
15 RIDLEY, D.S. Bacterial indices. In:COCHRANE,R.G. &DAVEY, T. Leprosy in theory and practice, 2nd ed. Bristol: John Wright & Sons, 1964, 659p.
16 SHEPARD, C.C. & McRAE, D.H. A method for counting acid fast bacteria. Int. J. Lepr., 36:78-82, 1968.
17 UNDP/WORLD BANK/WHO. Special pro- gramme for Research and Training in Tropical Diseases. IMMLEP. Recom- mended safety requirements for the preparation of lepromin: a WHO memorandum. Bulletin of World Health Organization, 57(6):921- 923, 1979.
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Publicado
30-11-1991
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1.
MARTINS MC, PALACI M, UEKI SYM, FERRAZOLI L, SATO DN, ICHIKAWA T, COSTA HC. Microscopia de fluorescência como procedimento para a realização do exame baciloscôpico em hanseníase. Hansen. Int. [Internet]. 30º de novembro de 1991 [citado 5º de maio de 2024];16(1/2):29-34. Disponível em: https://periodicos.saude.sp.gov.br/hansenologia/article/view/35228
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