Palabras clave
Mycobacterium leprae
Microscopia
Colorações
Microscopia
Colorações
Cómo citar
1.
MARTINS MC, PALACI M, UEKI SYM, FERRAZOLI L, SATO DN, ICHIKAWA T, COSTA HC. Microscopia de fluorescência como procedimento para a realização do exame baciloscôpico em hanseníase. Hansen. Int. [Internet]. 30 de noviembre de 1991 [citado 22 de noviembre de 2024];16(1/2):29-34. Disponible en: https://periodicos.saude.sp.gov.br/hansenologia/article/view/35228
Resumen
Visando comparar os métodos de microscope fluorescente e de Ziehl-Neelsen para evidenciaçâo de bacilos resistentes em material proveniente de derme de pacientes hansenlanos, foram examinados 355 esfregaços. Obteve-se 98,81% de concordância ajustada (KAPPA)entre os métodos. Verificou-se no entanto aproximadamente 55% de resuftados discordantes em relação ao índice baciloscdpico (Ridley), decorrentes de contagens de bacilos por campo mais elevadas pelo método fluorescente.
Citas
1 BRASIL Ministério da Saúde. Secretaria Nacional de Programas Especiais de Saúde. DMsão Nacional de Dermatologia Sanitária. Normas técnicas e procedimentos para o exame baciloscópio em hanseníase. Brasilia, 1989, 32p.
2 COLLINS, N.M.; MORRISON, N.E.; DHOPLE, A.M.; WATSON, S.R. Microscopic counts carried out on Mycobacterium leprae and
Mycobacterium tuberculosis suspensions. A comparison of three stain- ing procedures. Int. J. Lepr., 48(4):402-407,1980.
3 COSTA, H.C.; SOUZA, LCD.; MARTINI, J.P.; FERRAZOLI, L MARTINS, M.O.; OPROMOLLA, D.V.A.; DEL GIUDICE, A. C. Estudo comparativo das variantes do método de coloração de micobactórias. Hansen. Int., 13(2):37- 41, 1988.
4 FARIA, J.L. Fluorescent staining for Mycobacterium leprae in tissue sec- tions comparison with Fite- Faraco Procedure. Int. J. Lepr., 42:52-54, 1974.
5 DUBOIS, A. & SWERTZ, L L'emploi du micro- scope a fluorescence dans le diagnostic de la lepre. Ann. Soc. Belg. Med. Trop., 30:1473, 1950 (also abstracted In Int. J. Lepr., 19:260, 1951).
6 HAGEMANN, P.K.H. Fluorescence of Mycobacteria with auramine. Muchen Med. Wschr., 85:1066, 1938.
7 HANKS, J.H.; CHATTERGEE, M.B.; LECHAT, M.F. A guide to the couting ofmycobacteria in clinical and experimental materials. Int. J. Lepr., 32(2): 156- 167, 1964.
8 HARDAS, V. & LELE, V. Evaluation of fluorescent microscopy for detection of Mycobacterium leprae. Lepr. India, 53(2):273-277, 1981.
9 JARIWALA, H.J. & KELKAR, S.S. Fluores- cence microscopy for detection of M. leprae in tissue sections. Int. J. Lepr., 47(1):33-36, 1979.
10 KUPER, S.W.A. & MAY, JR. Detection of acid-fast organisms in tissue sections by fluorescence microscopy. J. Path. Beet., 79:59-68, 1960.
11 LEIKER, D.L & McDOUGALL, A.C. Technical guide for smear examination for leprosy by direct microscopy. Infolep-Royal Tropical
institute. Amsterdam, p.35.
12 MANSFIELD, R.E. An improved method for the fluorochrome staining of mycobacteria in tissues and smears. Am. J. Olin. Pathol.,
53:394-406, 1970.
13 PRENDES, M.A.G.; CARBONELE, A.F.; PARDOCASTELL0, V.; HERNANDES, A.C. La microscopia fluorescente em le prolog la .
Valoracion del metodo fluorescente frente al Ziehl-Neelsen en el diagnóstico bacteriológico. Int. J. Lepr., 21(1):35- 40, 1953.
14 RICHARDS, O.W. & MILLER, D.K. An efficient method for the identification of tuberculosis bacteria with a simple fluorescent microscope. Am. J. Win. Pathol. Tech. Suppl., 5:1-8, 1941.
15 RIDLEY, D.S. Bacterial indices. In:COCHRANE,R.G. &DAVEY, T. Leprosy in theory and practice, 2nd ed. Bristol: John Wright & Sons, 1964, 659p.
16 SHEPARD, C.C. & McRAE, D.H. A method for counting acid fast bacteria. Int. J. Lepr., 36:78-82, 1968.
17 UNDP/WORLD BANK/WHO. Special pro- gramme for Research and Training in Tropical Diseases. IMMLEP. Recom- mended safety requirements for the preparation of lepromin: a WHO memorandum. Bulletin of World Health Organization, 57(6):921- 923, 1979.
2 COLLINS, N.M.; MORRISON, N.E.; DHOPLE, A.M.; WATSON, S.R. Microscopic counts carried out on Mycobacterium leprae and
Mycobacterium tuberculosis suspensions. A comparison of three stain- ing procedures. Int. J. Lepr., 48(4):402-407,1980.
3 COSTA, H.C.; SOUZA, LCD.; MARTINI, J.P.; FERRAZOLI, L MARTINS, M.O.; OPROMOLLA, D.V.A.; DEL GIUDICE, A. C. Estudo comparativo das variantes do método de coloração de micobactórias. Hansen. Int., 13(2):37- 41, 1988.
4 FARIA, J.L. Fluorescent staining for Mycobacterium leprae in tissue sec- tions comparison with Fite- Faraco Procedure. Int. J. Lepr., 42:52-54, 1974.
5 DUBOIS, A. & SWERTZ, L L'emploi du micro- scope a fluorescence dans le diagnostic de la lepre. Ann. Soc. Belg. Med. Trop., 30:1473, 1950 (also abstracted In Int. J. Lepr., 19:260, 1951).
6 HAGEMANN, P.K.H. Fluorescence of Mycobacteria with auramine. Muchen Med. Wschr., 85:1066, 1938.
7 HANKS, J.H.; CHATTERGEE, M.B.; LECHAT, M.F. A guide to the couting ofmycobacteria in clinical and experimental materials. Int. J. Lepr., 32(2): 156- 167, 1964.
8 HARDAS, V. & LELE, V. Evaluation of fluorescent microscopy for detection of Mycobacterium leprae. Lepr. India, 53(2):273-277, 1981.
9 JARIWALA, H.J. & KELKAR, S.S. Fluores- cence microscopy for detection of M. leprae in tissue sections. Int. J. Lepr., 47(1):33-36, 1979.
10 KUPER, S.W.A. & MAY, JR. Detection of acid-fast organisms in tissue sections by fluorescence microscopy. J. Path. Beet., 79:59-68, 1960.
11 LEIKER, D.L & McDOUGALL, A.C. Technical guide for smear examination for leprosy by direct microscopy. Infolep-Royal Tropical
institute. Amsterdam, p.35.
12 MANSFIELD, R.E. An improved method for the fluorochrome staining of mycobacteria in tissues and smears. Am. J. Olin. Pathol.,
53:394-406, 1970.
13 PRENDES, M.A.G.; CARBONELE, A.F.; PARDOCASTELL0, V.; HERNANDES, A.C. La microscopia fluorescente em le prolog la .
Valoracion del metodo fluorescente frente al Ziehl-Neelsen en el diagnóstico bacteriológico. Int. J. Lepr., 21(1):35- 40, 1953.
14 RICHARDS, O.W. & MILLER, D.K. An efficient method for the identification of tuberculosis bacteria with a simple fluorescent microscope. Am. J. Win. Pathol. Tech. Suppl., 5:1-8, 1941.
15 RIDLEY, D.S. Bacterial indices. In:COCHRANE,R.G. &DAVEY, T. Leprosy in theory and practice, 2nd ed. Bristol: John Wright & Sons, 1964, 659p.
16 SHEPARD, C.C. & McRAE, D.H. A method for counting acid fast bacteria. Int. J. Lepr., 36:78-82, 1968.
17 UNDP/WORLD BANK/WHO. Special pro- gramme for Research and Training in Tropical Diseases. IMMLEP. Recom- mended safety requirements for the preparation of lepromin: a WHO memorandum. Bulletin of World Health Organization, 57(6):921- 923, 1979.
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