Identificação específica do M. leprae pela técnica da Reação em Cadeia da Polimerase(PCR)

Autores

  • Cristine HACKEL Profa. Assistente Doutora do Departamento de Genética Médica da Faculdade de Ciências Médicas da UNICAMP.

DOI:

https://doi.org/10.47878/hi.1990.v15.35504

Palavras-chave:

Polimerase, Reação em cadeia, M, Leprae, DNA, Identificação

Resumo

A autora descreve a metodologia baseada na reação em cadeia da polimerase (PCR) para identificação especifica do DNA de Mycobacterium leprae e apresenta uma revisão das estratégias relacionadas à escolha dos oligonucleotfdios iniciadores de síntese do DNA ("primers") e dos critérios de especificidade e sensibilidade necessários à utilização desse método em estudos clínicos e experimentais

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Referências

1. ANDERSEN, A.B & HANSEN, E.B. Structure and mapping of antigenic domains of protein antigen b, a 38,000-molecular protein of Mycobacterium tuberculosis. Infect. Immun.. 57:2481-2488, 1989.
2. BAIRD, P.N.; HALL, L.M.C.,COATES, A.R. Cloning and sequence analysis of the 10KDa antigen gene of Mycobacterium tuberculosis. J.Gen.Microbiol.. 135:931-939, 1989.
3. BOOTH, R.J.; HARRIS, D.P.; LOVE, J.M.; WATSON, J.D. Antigenic proteins of Mycobacterium leprae: complete sequence of the gene
for the 18-KDa protein. J.Immunol., 140:597-601, 1988.
4. CHIEN, A.; EDGAR, D.B.; TRELA, J.M. Desoxyribonucleic acid polymerase from the extreme thermophile thermus aquaticus. J. Bacteriol..127:1550-1557, 1976.
5. GARSIA, R.J.; HELLOVIST, L.; BOOTH, R.J.; RADFORD, A.J.; BRITTON, W.J.; ASTBURY,L.;TRENT, R.J; BASTEN, A.Homology of the 70-kilodalton antigens from Mycobacterium leprae and Mycobacterium bovis with the Mycobacterium tuberculosis 71-kilodalton antigen and with the conserved heat shock proteins 70 of eucaryoter. Infect, Immun.. 57:204-212, 1989.
8. HACKEL, C.; HOUARD,S.;PORTAELS, F.; VAN ELSEN,A.; HERZOG,A.; BOLLEN,A. Specific identification of Mycobacterium leprae by the polymerase chain reaction. Mol. Cell. Probes. 4:205-210, 1990
9. HARTSKEERL, R. A.; DE WIT, M.Y.L.; KLASTSER, P.R. Polymerase chain reaction for the detection of Mycobacterium leprae. Gen. Microbiol..135:2357-2364, 1989.
10. HARTSKEERL, R.A.; STABEL, L.F.E.M.; HERMANS, C.J.; KLASTSER, P.R; THOLE, J.E.R. Nucleotide and deduced aminoacid sequence of a Mycobacterium leprae 12K protein. Nucleic Acid Res.. 8:1294, 1990.
6. KLEPPE, K.; OHTSUKA, E., KLEPPE, E., MOLINEUX, I., KHORANA, H.C. Studies on polynucleotides XCVI: repairreplication of short synthetic DNAs as catalyzed by DNA polymuse. J. Mol.Biol. 56:341-361, 1971
10. KOGAN,S.C; DOHERTY,M.,GITSCHIER,J. An improved method for prenatal diagnosis of genetic diseases by analysis of amplified DNA sequences. N. Engl. J. Med.. 317:985-990, 1987.
11. KW OK, S. Procedures to minimize PCR product carry over. In: PCR PROTOCOLS. A guide to methods and applications. San Diego, Academic Press, 1990. 482p.
12. LI, H; CYLLENSTEN, U.B; CUI, X.; SAIKI, R.K.; BRLICH, N.A.; ARNHEIM, N. aplication and analysis of DNA sequences in single human sperm and diploid cells. Nature. 335:414-417,1988
13. HATSUO, K.; YAMAGUCHI, R.; YAMAZAKI, A.; TASAKA, G.; YAMADA, T. Cloning and expression of the Mycobacterium bovis BCG gene for extracellular antigen. J. Bacteriol.. 170:3847-3854, 1988.
14. MEHRA,V.; SWEETSER,C.; YOUNG, R.A Efficient mapping of protein antigenic determinants. Proc: Natl. Acad. Sci., 83:7013-7017, 1986.
15. PLIKAYTIS, B. B.; GELBER, R. H.; SHINNICK, T.M. Rapid and sensitive detection of Mycobacterium Ieprae using a nested primer gene amplification assay. J. Clin. Microbiol.. 28:1913-1917, 1990.
16. PORTAELS, F.; FISSETE, K.; DE RIDDER, K.; MACEDO, P.M.; DE MUYNCK, A.; SILVA, M.T. Effects of freezing and thawing on the viability and the ultrastructure of in vivo grown mycobacteria. Int. J. Lepr.. 56:5805 87, 1988.
17. REISS, J. & COOPER, D.N. Application of the polymerase chain reaction to thediagnosis of human genetic disease. Ilumen Genetics. 85:1-8, 1990.
18. SAIKI, R.K; SCHARF, S.; FALOONA, F.; MULLIS, K.D.; HORN, C.T.; ERLICH, H.A.; ARHNEIM, N. Enzymatic amplification of globin genomic sequences and restriction site analysis for diagnosis of sickle celi anemia. Science. 230:1350-1354,1985.
19. SAIKI,R.K.; CELFAND,D.H.; STOFFEL,S.; SCHAFF, S.J.; HIGUCHI, R. HORN, C.T.; MULLIS, K.B.; ERLICH, H.A. Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science. 239:487-491, 1988
20. SALEM,J.I.; CADELHA, A.R.; MAROJA,F.; DAVID, H.L. Non cultivable mycobacteria in ulcers of the skin. Acta Leprol.. 7 (Suppl.l ):10-55, 1989.
21. SALEM, J.I.; CONTIJO FILHO, P.; LEVY FREDAULT, V.; DAVID, H.L. Isolation and characterization of mycobacteria colonizing the healthy skin. Acta Leprol.. 7 (Suppl.1):18-20, 1989.
22. SHINNICK, J.I.; CONTIJO FILHO, P.; LEVY FREDAULT, V.; DAVID, H.L. Isolation and characterization of micobacteria colonizing the healthy skin. Acta Leprol.. 7(Suppl.1):18-20 1989.
23. SHINNICK,T.M,; SWEETSER, D.; THOLE,J.; VAN EMBDEN, J.; EONG, R.A. The etiologic agents of leprosy and tuberculosis share an immunoreactive protein antigen with the vaccine strain Mycobacterium bovis BCG. Infect. Immun.. 55:1932-1935, 1987.
24. SHINNICK, T.M,; PLIKATIS, B.B.; HYCHE, A.D.; VAN LANDIGHAM, R.M.; WALKER, L.L. Mycobacterium turberculosis BCG a protein has homology with the Escherichla coil Groes protein. Nucleic Acid Res.. 17:1254, 1989.
25. STACKEMDRANDT, E.; SMIDA,; KAZDA,J. The primary structure of the 16SrRna of Mycobacterium leprae: its use in phylogeny and development of •NA probes. Acta Leprol.. 7(Suppl. 1):222-225, 1989.
26. SUZUKI, Y.; NAGATA, A.; ONO, Y.; YAMADA, T. Complete nucleotide sequence of the 16SrRNA gene of Mycobacterium bovis B C G, J.Bacteriol.. 170:2886-1889, 1988.
27. TRANOCARAJ, H.S.; LAMB, F.I.; DAVIS, E.O.; JENNER, P.J., JEYAKUMAR, L.H., COLSTON, M.J. Identification, sequencing, and expression of Mycobacterium leprae superoxide dismutase, a major antigen. Infect. Immun. 58:1937-1942, 1990.
28. THOLE, J.E.R.; KEULEN, W.J.; KOLK, A.H.J.; GROOTHUIS, D.C; BERWALD, L.G.;TIESJEMA„ R.H..; VAN EMBDEN,J.D.A. Characterization,
sequence determination, and immunogenicity of a 64 kilodalton protein of Mycobacterium bovis BCG expressed in Escherichia coil K-12. Infect. Immun.55:1466 1475, 1987.
29. THOKE, J.E.; STADEL, L.F.; SUYKERBUYK, D.E.; DE WITH, M.Y.; KLATSER, P.R.; KOLK, a.h.; HARTSKEERL, R.A. A major immunogenic 36000 molecular antigen from Mycobacterium leprae contains an immunoreative region of proline- rich repeats. Infect. Immun.
58:80-87, 1990.

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Publicado

30-11-1990

Como Citar

1.
HACKEL C. Identificação específica do M. leprae pela técnica da Reação em Cadeia da Polimerase(PCR). Hansen. Int. [Internet]. 30º de novembro de 1990 [citado 16º de julho de 2024];15(1/2):67-75. Disponível em: https://periodicos.saude.sp.gov.br/hansenologia/article/view/35504

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