Identificação específica do M. leprae pela técnica da Reação em Cadeia da Polimerase(PCR)
DOI:
https://doi.org/10.47878/hi.1990.v15.35504Keywords:
Polymerase, M.leprae DNA, IdentificationAbstract
The author describes the methodology based on the polymerase chain reaction (PCR) for the specific detection of ycobacterium leprae DNA, by reviewing the approaches concerning the choice of oligonucleotide primers and the criteria of specificity and sensitivity required for a useful tool in clinical and experimental studies.
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References
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2. BAIRD, P.N.; HALL, L.M.C.,COATES, A.R. Cloning and sequence analysis of the 10KDa antigen gene of Mycobacterium tuberculosis. J.Gen.Microbiol.. 135:931-939, 1989.
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8. HACKEL, C.; HOUARD,S.;PORTAELS, F.; VAN ELSEN,A.; HERZOG,A.; BOLLEN,A. Specific identification of Mycobacterium leprae by the polymerase chain reaction. Mol. Cell. Probes. 4:205-210, 1990
9. HARTSKEERL, R. A.; DE WIT, M.Y.L.; KLASTSER, P.R. Polymerase chain reaction for the detection of Mycobacterium leprae. Gen. Microbiol..135:2357-2364, 1989.
10. HARTSKEERL, R.A.; STABEL, L.F.E.M.; HERMANS, C.J.; KLASTSER, P.R; THOLE, J.E.R. Nucleotide and deduced aminoacid sequence of a Mycobacterium leprae 12K protein. Nucleic Acid Res.. 8:1294, 1990.
6. KLEPPE, K.; OHTSUKA, E., KLEPPE, E., MOLINEUX, I., KHORANA, H.C. Studies on polynucleotides XCVI: repairreplication of short synthetic DNAs as catalyzed by DNA polymuse. J. Mol.Biol. 56:341-361, 1971
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12. LI, H; CYLLENSTEN, U.B; CUI, X.; SAIKI, R.K.; BRLICH, N.A.; ARNHEIM, N. aplication and analysis of DNA sequences in single human sperm and diploid cells. Nature. 335:414-417,1988
13. HATSUO, K.; YAMAGUCHI, R.; YAMAZAKI, A.; TASAKA, G.; YAMADA, T. Cloning and expression of the Mycobacterium bovis BCG gene for extracellular antigen. J. Bacteriol.. 170:3847-3854, 1988.
14. MEHRA,V.; SWEETSER,C.; YOUNG, R.A Efficient mapping of protein antigenic determinants. Proc: Natl. Acad. Sci., 83:7013-7017, 1986.
15. PLIKAYTIS, B. B.; GELBER, R. H.; SHINNICK, T.M. Rapid and sensitive detection of Mycobacterium Ieprae using a nested primer gene amplification assay. J. Clin. Microbiol.. 28:1913-1917, 1990.
16. PORTAELS, F.; FISSETE, K.; DE RIDDER, K.; MACEDO, P.M.; DE MUYNCK, A.; SILVA, M.T. Effects of freezing and thawing on the viability and the ultrastructure of in vivo grown mycobacteria. Int. J. Lepr.. 56:5805 87, 1988.
17. REISS, J. & COOPER, D.N. Application of the polymerase chain reaction to thediagnosis of human genetic disease. Ilumen Genetics. 85:1-8, 1990.
18. SAIKI, R.K; SCHARF, S.; FALOONA, F.; MULLIS, K.D.; HORN, C.T.; ERLICH, H.A.; ARHNEIM, N. Enzymatic amplification of globin genomic sequences and restriction site analysis for diagnosis of sickle celi anemia. Science. 230:1350-1354,1985.
19. SAIKI,R.K.; CELFAND,D.H.; STOFFEL,S.; SCHAFF, S.J.; HIGUCHI, R. HORN, C.T.; MULLIS, K.B.; ERLICH, H.A. Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science. 239:487-491, 1988
20. SALEM,J.I.; CADELHA, A.R.; MAROJA,F.; DAVID, H.L. Non cultivable mycobacteria in ulcers of the skin. Acta Leprol.. 7 (Suppl.l ):10-55, 1989.
21. SALEM, J.I.; CONTIJO FILHO, P.; LEVY FREDAULT, V.; DAVID, H.L. Isolation and characterization of mycobacteria colonizing the healthy skin. Acta Leprol.. 7 (Suppl.1):18-20, 1989.
22. SHINNICK, J.I.; CONTIJO FILHO, P.; LEVY FREDAULT, V.; DAVID, H.L. Isolation and characterization of micobacteria colonizing the healthy skin. Acta Leprol.. 7(Suppl.1):18-20 1989.
23. SHINNICK,T.M,; SWEETSER, D.; THOLE,J.; VAN EMBDEN, J.; EONG, R.A. The etiologic agents of leprosy and tuberculosis share an immunoreactive protein antigen with the vaccine strain Mycobacterium bovis BCG. Infect. Immun.. 55:1932-1935, 1987.
24. SHINNICK, T.M,; PLIKATIS, B.B.; HYCHE, A.D.; VAN LANDIGHAM, R.M.; WALKER, L.L. Mycobacterium turberculosis BCG a protein has homology with the Escherichla coil Groes protein. Nucleic Acid Res.. 17:1254, 1989.
25. STACKEMDRANDT, E.; SMIDA,; KAZDA,J. The primary structure of the 16SrRna of Mycobacterium leprae: its use in phylogeny and development of •NA probes. Acta Leprol.. 7(Suppl. 1):222-225, 1989.
26. SUZUKI, Y.; NAGATA, A.; ONO, Y.; YAMADA, T. Complete nucleotide sequence of the 16SrRNA gene of Mycobacterium bovis B C G, J.Bacteriol.. 170:2886-1889, 1988.
27. TRANOCARAJ, H.S.; LAMB, F.I.; DAVIS, E.O.; JENNER, P.J., JEYAKUMAR, L.H., COLSTON, M.J. Identification, sequencing, and expression of Mycobacterium leprae superoxide dismutase, a major antigen. Infect. Immun. 58:1937-1942, 1990.
28. THOLE, J.E.R.; KEULEN, W.J.; KOLK, A.H.J.; GROOTHUIS, D.C; BERWALD, L.G.;TIESJEMA„ R.H..; VAN EMBDEN,J.D.A. Characterization,
sequence determination, and immunogenicity of a 64 kilodalton protein of Mycobacterium bovis BCG expressed in Escherichia coil K-12. Infect. Immun.55:1466 1475, 1987.
29. THOKE, J.E.; STADEL, L.F.; SUYKERBUYK, D.E.; DE WITH, M.Y.; KLATSER, P.R.; KOLK, a.h.; HARTSKEERL, R.A. A major immunogenic 36000 molecular antigen from Mycobacterium leprae contains an immunoreative region of proline- rich repeats. Infect. Immun.
58:80-87, 1990.
2. BAIRD, P.N.; HALL, L.M.C.,COATES, A.R. Cloning and sequence analysis of the 10KDa antigen gene of Mycobacterium tuberculosis. J.Gen.Microbiol.. 135:931-939, 1989.
3. BOOTH, R.J.; HARRIS, D.P.; LOVE, J.M.; WATSON, J.D. Antigenic proteins of Mycobacterium leprae: complete sequence of the gene
for the 18-KDa protein. J.Immunol., 140:597-601, 1988.
4. CHIEN, A.; EDGAR, D.B.; TRELA, J.M. Desoxyribonucleic acid polymerase from the extreme thermophile thermus aquaticus. J. Bacteriol..127:1550-1557, 1976.
5. GARSIA, R.J.; HELLOVIST, L.; BOOTH, R.J.; RADFORD, A.J.; BRITTON, W.J.; ASTBURY,L.;TRENT, R.J; BASTEN, A.Homology of the 70-kilodalton antigens from Mycobacterium leprae and Mycobacterium bovis with the Mycobacterium tuberculosis 71-kilodalton antigen and with the conserved heat shock proteins 70 of eucaryoter. Infect, Immun.. 57:204-212, 1989.
8. HACKEL, C.; HOUARD,S.;PORTAELS, F.; VAN ELSEN,A.; HERZOG,A.; BOLLEN,A. Specific identification of Mycobacterium leprae by the polymerase chain reaction. Mol. Cell. Probes. 4:205-210, 1990
9. HARTSKEERL, R. A.; DE WIT, M.Y.L.; KLASTSER, P.R. Polymerase chain reaction for the detection of Mycobacterium leprae. Gen. Microbiol..135:2357-2364, 1989.
10. HARTSKEERL, R.A.; STABEL, L.F.E.M.; HERMANS, C.J.; KLASTSER, P.R; THOLE, J.E.R. Nucleotide and deduced aminoacid sequence of a Mycobacterium leprae 12K protein. Nucleic Acid Res.. 8:1294, 1990.
6. KLEPPE, K.; OHTSUKA, E., KLEPPE, E., MOLINEUX, I., KHORANA, H.C. Studies on polynucleotides XCVI: repairreplication of short synthetic DNAs as catalyzed by DNA polymuse. J. Mol.Biol. 56:341-361, 1971
10. KOGAN,S.C; DOHERTY,M.,GITSCHIER,J. An improved method for prenatal diagnosis of genetic diseases by analysis of amplified DNA sequences. N. Engl. J. Med.. 317:985-990, 1987.
11. KW OK, S. Procedures to minimize PCR product carry over. In: PCR PROTOCOLS. A guide to methods and applications. San Diego, Academic Press, 1990. 482p.
12. LI, H; CYLLENSTEN, U.B; CUI, X.; SAIKI, R.K.; BRLICH, N.A.; ARNHEIM, N. aplication and analysis of DNA sequences in single human sperm and diploid cells. Nature. 335:414-417,1988
13. HATSUO, K.; YAMAGUCHI, R.; YAMAZAKI, A.; TASAKA, G.; YAMADA, T. Cloning and expression of the Mycobacterium bovis BCG gene for extracellular antigen. J. Bacteriol.. 170:3847-3854, 1988.
14. MEHRA,V.; SWEETSER,C.; YOUNG, R.A Efficient mapping of protein antigenic determinants. Proc: Natl. Acad. Sci., 83:7013-7017, 1986.
15. PLIKAYTIS, B. B.; GELBER, R. H.; SHINNICK, T.M. Rapid and sensitive detection of Mycobacterium Ieprae using a nested primer gene amplification assay. J. Clin. Microbiol.. 28:1913-1917, 1990.
16. PORTAELS, F.; FISSETE, K.; DE RIDDER, K.; MACEDO, P.M.; DE MUYNCK, A.; SILVA, M.T. Effects of freezing and thawing on the viability and the ultrastructure of in vivo grown mycobacteria. Int. J. Lepr.. 56:5805 87, 1988.
17. REISS, J. & COOPER, D.N. Application of the polymerase chain reaction to thediagnosis of human genetic disease. Ilumen Genetics. 85:1-8, 1990.
18. SAIKI, R.K; SCHARF, S.; FALOONA, F.; MULLIS, K.D.; HORN, C.T.; ERLICH, H.A.; ARHNEIM, N. Enzymatic amplification of globin genomic sequences and restriction site analysis for diagnosis of sickle celi anemia. Science. 230:1350-1354,1985.
19. SAIKI,R.K.; CELFAND,D.H.; STOFFEL,S.; SCHAFF, S.J.; HIGUCHI, R. HORN, C.T.; MULLIS, K.B.; ERLICH, H.A. Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science. 239:487-491, 1988
20. SALEM,J.I.; CADELHA, A.R.; MAROJA,F.; DAVID, H.L. Non cultivable mycobacteria in ulcers of the skin. Acta Leprol.. 7 (Suppl.l ):10-55, 1989.
21. SALEM, J.I.; CONTIJO FILHO, P.; LEVY FREDAULT, V.; DAVID, H.L. Isolation and characterization of mycobacteria colonizing the healthy skin. Acta Leprol.. 7 (Suppl.1):18-20, 1989.
22. SHINNICK, J.I.; CONTIJO FILHO, P.; LEVY FREDAULT, V.; DAVID, H.L. Isolation and characterization of micobacteria colonizing the healthy skin. Acta Leprol.. 7(Suppl.1):18-20 1989.
23. SHINNICK,T.M,; SWEETSER, D.; THOLE,J.; VAN EMBDEN, J.; EONG, R.A. The etiologic agents of leprosy and tuberculosis share an immunoreactive protein antigen with the vaccine strain Mycobacterium bovis BCG. Infect. Immun.. 55:1932-1935, 1987.
24. SHINNICK, T.M,; PLIKATIS, B.B.; HYCHE, A.D.; VAN LANDIGHAM, R.M.; WALKER, L.L. Mycobacterium turberculosis BCG a protein has homology with the Escherichla coil Groes protein. Nucleic Acid Res.. 17:1254, 1989.
25. STACKEMDRANDT, E.; SMIDA,; KAZDA,J. The primary structure of the 16SrRna of Mycobacterium leprae: its use in phylogeny and development of •NA probes. Acta Leprol.. 7(Suppl. 1):222-225, 1989.
26. SUZUKI, Y.; NAGATA, A.; ONO, Y.; YAMADA, T. Complete nucleotide sequence of the 16SrRNA gene of Mycobacterium bovis B C G, J.Bacteriol.. 170:2886-1889, 1988.
27. TRANOCARAJ, H.S.; LAMB, F.I.; DAVIS, E.O.; JENNER, P.J., JEYAKUMAR, L.H., COLSTON, M.J. Identification, sequencing, and expression of Mycobacterium leprae superoxide dismutase, a major antigen. Infect. Immun. 58:1937-1942, 1990.
28. THOLE, J.E.R.; KEULEN, W.J.; KOLK, A.H.J.; GROOTHUIS, D.C; BERWALD, L.G.;TIESJEMA„ R.H..; VAN EMBDEN,J.D.A. Characterization,
sequence determination, and immunogenicity of a 64 kilodalton protein of Mycobacterium bovis BCG expressed in Escherichia coil K-12. Infect. Immun.55:1466 1475, 1987.
29. THOKE, J.E.; STADEL, L.F.; SUYKERBUYK, D.E.; DE WITH, M.Y.; KLATSER, P.R.; KOLK, a.h.; HARTSKEERL, R.A. A major immunogenic 36000 molecular antigen from Mycobacterium leprae contains an immunoreative region of proline- rich repeats. Infect. Immun.
58:80-87, 1990.
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Published
1990-11-30
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HACKEL C. Identificação específica do M. leprae pela técnica da Reação em Cadeia da Polimerase(PCR). Hansen. Int. [Internet]. 1990 Nov. 30 [cited 2024 May 18];15(1/2):67-75. Available from: https://periodicos.saude.sp.gov.br/hansenologia/article/view/35504
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