Palavras-chave
Hanseníase
ELISA
Glicolipídeo fenólico-1-PGL-1
ELISA
Glicolipídeo fenólico-1-PGL-1
Como Citar
1.
Brasil MTL, Oliveira LR de, Mello CS de, Nakamura PM, Manini MP, teiner D, Rotta O. Estudo da sensibilidade e especificidade do teste Elisa Anti PGL-1 no estado de São Paulo. Hansen. Int. [Internet]. 30º de novembro de 1997 [citado 26º de dezembro de 2024];22(2):35-43. Disponível em: https://periodicos.saude.sp.gov.br/hansenologia/article/view/36449
Resumo
Os testes sorológicos para diagnóstico de hanseníase, usando o glicolipídeofenólico, considerado antígeno específico do M. leprae têm aberto algumas possibilidades de estudo do comportamento epidemiológico desta doença. Foi realizado um estudo para medir a sensibilidade e especificidade de um teste de ELISA anti PGL-1, usando material e técnica proveniente de Cuba (UMELISA). Foram testados 84 doentes e 112 controles sadios. A sensibilidade do teste foi maior para o grupo de doentes multibacilares, sendo mais alto entre os casos classificados como virchowianos (V), seguido dos dimorfos (D). No grupo de multibacilares, considerando o limiar de reatividade de 0,200, observou-se que apenas 75,0% de pacientes V e 50,0% de D foram positivos ao teste e, no limiar de 0,300, apenas 64,3% dos V e 40,0% dos D ainda mostravam positividade. Os doentes indeterminados apresentaram maior proporção de soropositivos do que os tuberculóides e isto talvez traduza a polarização de alguns casos para formas multibacilares. A especificidade do teste foi de 87,5% no limiar de 0,200 e de 99,1% no de 0,300. Este teste, à semelhança de outros, apresentou alta especificidade e baixa sensibilidade, resultando em grande percentual de falso-negativos. Para uma doença em que os testes sorológicos são escassos, apesar de não se recomendar o uso indiscriminado do teste para triagem de casos na população geral, a continuidade de seu aprimoramento tecnológico deve ser estimulada. Conseguir-se-ia, assim, avançar na pesquisa de instrumentos cada vez mais sensíveis e específicos para melhorar o diagnóstico precoce e, desse modo intervir mais oportunamente na cadeia de transmissão.
Referências
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ABREU-CASTELLS,E.G.;SEGREDO,A .C.;ALBERNAS, J. M.; BENCOMO,V.V. Comprobacion serologica de la actividad de um antigeno del M. leprae obtenido por sistesis quimica. Revista cubana - Medicina Tropical, v. 41(1), p. 10-17,1989.
BACH, M.A. ; WALLACH, D. ; FLAGEUL, B. ; et al . Antibodies to Phenolic Glycolipid-1 and to whole M. Leprae in leprosy patients: evolution during
therapy . int. J. Lepr. Other Mycobad. Dis., v. 54 (2), p. 256-276, 1986.
BRENNAN,P. J . & BARROW, W.W. - Evidence for species-specific lipid antigen of Mycobacterium leprae. Int. J. Lepr. Other Mycobact. Dis., v. 48, p. 382- 387, 1981.
BRETT,S.J.; DRAPER, P.; PAYNE,S.N.; REES,J.W. Serological activity of a characteristic phenolic glycolipid from M. leprae in sera from patients wi th leprosy and tuberculos is. Cl in. Exp. Immunol., v. 52, p. 271-279, 1983.BRETT, S.J.; PAYNE, S.N.; GIGG, J.; et al.Use of synthetic and immuno-dominant epitope of phenolic glycolipid-1 serology of leprosy. Clin. Exp. Immunol., v. 64, p. 476-483, 1986.
BUCHANAN, T.M.; YOUNG, D.B.; MILLER, R.a; KHANOLKAR, S.R. Serodiagnosis of infection with Mycobacterium leprae. Int. J. Lepr. Other Mycobact. Dis., v. 51(4), p. 524-530, 1983.
BURGESS,P.J.; FINE, P.E.M.; PONNINGHAUS,J.M.; DRAPER,C. Serological tests in leprosy. The sensitivity, specificity and predictive value of ELISA tests based on phenolic glycolipid antigens and the
implications for their use in epidemiological studies. Epidem. Inf.,v. 101, p. 159-171, 1988.
CALDWELL,H.D.; KIRCHHEIMER,W.F.; BUCHANAN, T.M. Identification of a Mycobacterium leprae specific protein antigen(s) and its possible aplication for the serodiagnosis of leprosy. Int. J. Lepr. Other Mycobact. Dis., v. 47(3): 477-483, 1979.
C HO, S . ; Y A N A G I H A R A , D. L . ; H U N T E R , S .W. ; GE LBE R, R.H. ; BRENNAN, P . J . Serological specificity of phenolic glycolipid-1 from Mycobacterium leprae and use in serodiagnosis of leprosy. Infectious and Immunity, v. 41(3), p. 1077-1083, 1983.
COPELAND, K.T.; CHECKOWAY,H.; McMICHAEL, A., J.; HOLBROOK, R.H. Bias due to misclassification in the estimation of relative risk. Am.J.
Epidem., v. 105(5), p. 488-495, 1977.
DAVID,H.L.; PAPA,F.; CRUAD,P.; et al. Relationship between titers of antibodies immunoreacting against glycolipid antigens from Mycobacterium
leprae and M. tuberculosis, tha Mitsuda and Mantoux reactions and bacteriological loads: implications in the pathogens, epidemiology and
serodiagnosis of leprosy and tuberculosis. Int. J. Lepr. Other Mycobact. Dis., v. 60 (2), p. 208-224, 1992.
DOUGLAS,J.T.; STEVEN,L.M.; FAJARDO, T.; et al. The effects of chemo-therapy on antibody levels in lepromatous patients. Lepr. Rev, v. 59, p. 127-135, 1988.
DOUGLAS,J.T.; STEVEN, L.M.; HIRCH,D.S.; Evaluation of four semisynthetic Mycobacterium leprae antigens with sera from healthy population in
endemic area. Lepr. Rev, v. 63, p.199-210, 1992.
FEINSTEIN, A . R. Clinical Epidemiology - part 6 - 601-609. W. B. Sanders Company. USA, 1985
FINE,P.E.M. - Leprosy: The epidemiology of a slow bacterium. Epidem. Rev., v. 4, p. 161-188, 1982.
FOSS,N.T.; CALLERA,F.; ALBERTO, F.L. Anti PGL-1 levels in leprosy patients and their contacts. Braz. J. Med. Biol. Res., v. 26, p. 43-51, 1993.
FUJIWARA,T. HUNTER,S.W. ; CHO,S.; ASPINAL,G.O .; BRENNAN,P.J. Chemical synthesis and serology of dissacharides and trissacharides of phenolic glycolipid antigens from the Leprosy bacillus and preparations of a dissacharide protein conjugate for serodiagnosis of leprosy. Infection and Immunity, v. 43(10), p.245-252, 1984.
HARBOE,M.; CLOSS,º BJUNG, G.; KRONVALL,G.; AXELSEN,N.H.- Mycobacterium leprae specific antibodies detected by radio immunoassay. Scand. J. Immunol., v. 7, p. 11-20, 1978.
HUNTER,S.W.; BRENNAN,P. J. A novel Phenolic Glycolipid from Mycobacterium leprae. Possible involved in immunogenicity and pathogenicity.
J. Bacteriol., v. 147(3), p. 728-735,1981.
HUNTER,S.W. ; FUGIWARA,T. ; BRENNAN,P.J. Structure and antigenicity of the major specific glycolipid antigen of Mycobacterium leprae. The Journal of Biological Chemistry, v. 257(24), p. 15072-15078, 1982.
KRONVALL,G. - The potential of immunological tests as a tool in the epidemiology of leprosy. Lepr. Rev., v. 52 (suppl 1), p. 207-219, 1981.
LAFERTE,J.; ABREU,E.G.; ROBAINA,R.; VEREZA, V. Ultramicroelisa para la detección de anticuerpos IgM anti M. leprae. Rev. Inst. Med. Trop. São
Paulo, v. 33(6), p. 491-495, 1991.
LANCET ( Anonymous) - Serological tests for leprosy. The Lancet, v. 8, p. 533- 535, 1986. (Editorial)
MENZEL,S.; HARBOE,M.; BERGSVIK,H.; BRENNAN, P.J. - Antibodies to a synthetic analog of phenolic glycolipid-1 of M. leprae in the healthy house-hold
contacts of patients with leprosy. Int. J. Lepr Other Mycobact. Dis., v. 55, p. 617-625, 1987.
PAYNE, N.S.;DRAPER,P.;REES,R.J.W.- Serological activity of purified glycolipid from Mycobacterium leprae. Int. J. Lepr. Other Mycobact. Dis., v. 50(2), p. 220-221, 1982.
REPKA,J.C.D.;BRANA,L.R.;FABRICIO,L.H.; et al. Imu-nologia na hanseníase - Aspectos de avaliação imunológica no tratamento. Arq. Biol. Tecnol., v.
34(1), p. 53-60, 1991.
SAAD,M.H.F.;MEDEIROS,M.A.; GALLO, M.E.N.; et al. IgM immunoglobulins reacting with the phenolic glycolipid-1 antigen from M. leprae in sera of
leprosy patients and contacts. Mem. Inst. O. Cruz, v. 85(2):191-194,1990.
SOEBONO,H. & KLATSER,P.R. - A seroepidemiological study of leprosy in high and low endemic Indonesian villages. Int. J. Lepr. Other Mycobact.
Dis., v. 59, 416-425, 1991.
TOUW,J.; LANGNDIJK, E.M.J.; STONER, G.L.; BELIHU,A. Humoral immunity in leprosy: immunoglobulin G and M antibody responses to Mycobacterium leprae in relation to various disease patterns. Infect. Immunit., v. 36(3), p. 885-892, 1982.
YAMASHITA, J.T.; CRUAD, P.; ROTTA, O.; DAVID, L.H.; - Circulating immune complexes in leprosy sera: demonstration of antibodies against mycobacterial glycolipid antigens in isolated immune complexes. Int. J. Lepr. Other Mycobact. Dis., v. 61(1), p.44-50,1993.
YOUNG, D.B.; DESSANAYAKE, S.; MILLER, R.A.; KHANOLKAR, S.; BUCHANAN, T.M. - Humans respond predominantly with IgM immunoglobulin to the species-specific glycolipid of Mycobacterium leprae. The Journal of Infectious Diseases, v. 149(6), p. 870-874, 1984.
YOUNG, D.B.; HARNISH, I.; KNIGHT, J.; BUCHANAN, T.M.- Detection of phenolic glycolipid-1 in sera from patients with lepromatous leprosy. The Journal of Infectious diseases, v. 152 (5),p. 1078-1081, 1985.
ABREU-CASTELLS,E.G.;SEGREDO,A .C.;ALBERNAS, J. M.; BENCOMO,V.V. Comprobacion serologica de la actividad de um antigeno del M. leprae obtenido por sistesis quimica. Revista cubana - Medicina Tropical, v. 41(1), p. 10-17,1989.
BACH, M.A. ; WALLACH, D. ; FLAGEUL, B. ; et al . Antibodies to Phenolic Glycolipid-1 and to whole M. Leprae in leprosy patients: evolution during
therapy . int. J. Lepr. Other Mycobad. Dis., v. 54 (2), p. 256-276, 1986.
BRENNAN,P. J . & BARROW, W.W. - Evidence for species-specific lipid antigen of Mycobacterium leprae. Int. J. Lepr. Other Mycobact. Dis., v. 48, p. 382- 387, 1981.
BRETT,S.J.; DRAPER, P.; PAYNE,S.N.; REES,J.W. Serological activity of a characteristic phenolic glycolipid from M. leprae in sera from patients wi th leprosy and tuberculos is. Cl in. Exp. Immunol., v. 52, p. 271-279, 1983.BRETT, S.J.; PAYNE, S.N.; GIGG, J.; et al.Use of synthetic and immuno-dominant epitope of phenolic glycolipid-1 serology of leprosy. Clin. Exp. Immunol., v. 64, p. 476-483, 1986.
BUCHANAN, T.M.; YOUNG, D.B.; MILLER, R.a; KHANOLKAR, S.R. Serodiagnosis of infection with Mycobacterium leprae. Int. J. Lepr. Other Mycobact. Dis., v. 51(4), p. 524-530, 1983.
BURGESS,P.J.; FINE, P.E.M.; PONNINGHAUS,J.M.; DRAPER,C. Serological tests in leprosy. The sensitivity, specificity and predictive value of ELISA tests based on phenolic glycolipid antigens and the
implications for their use in epidemiological studies. Epidem. Inf.,v. 101, p. 159-171, 1988.
CALDWELL,H.D.; KIRCHHEIMER,W.F.; BUCHANAN, T.M. Identification of a Mycobacterium leprae specific protein antigen(s) and its possible aplication for the serodiagnosis of leprosy. Int. J. Lepr. Other Mycobact. Dis., v. 47(3): 477-483, 1979.
C HO, S . ; Y A N A G I H A R A , D. L . ; H U N T E R , S .W. ; GE LBE R, R.H. ; BRENNAN, P . J . Serological specificity of phenolic glycolipid-1 from Mycobacterium leprae and use in serodiagnosis of leprosy. Infectious and Immunity, v. 41(3), p. 1077-1083, 1983.
COPELAND, K.T.; CHECKOWAY,H.; McMICHAEL, A., J.; HOLBROOK, R.H. Bias due to misclassification in the estimation of relative risk. Am.J.
Epidem., v. 105(5), p. 488-495, 1977.
DAVID,H.L.; PAPA,F.; CRUAD,P.; et al. Relationship between titers of antibodies immunoreacting against glycolipid antigens from Mycobacterium
leprae and M. tuberculosis, tha Mitsuda and Mantoux reactions and bacteriological loads: implications in the pathogens, epidemiology and
serodiagnosis of leprosy and tuberculosis. Int. J. Lepr. Other Mycobact. Dis., v. 60 (2), p. 208-224, 1992.
DOUGLAS,J.T.; STEVEN,L.M.; FAJARDO, T.; et al. The effects of chemo-therapy on antibody levels in lepromatous patients. Lepr. Rev, v. 59, p. 127-135, 1988.
DOUGLAS,J.T.; STEVEN, L.M.; HIRCH,D.S.; Evaluation of four semisynthetic Mycobacterium leprae antigens with sera from healthy population in
endemic area. Lepr. Rev, v. 63, p.199-210, 1992.
FEINSTEIN, A . R. Clinical Epidemiology - part 6 - 601-609. W. B. Sanders Company. USA, 1985
FINE,P.E.M. - Leprosy: The epidemiology of a slow bacterium. Epidem. Rev., v. 4, p. 161-188, 1982.
FOSS,N.T.; CALLERA,F.; ALBERTO, F.L. Anti PGL-1 levels in leprosy patients and their contacts. Braz. J. Med. Biol. Res., v. 26, p. 43-51, 1993.
FUJIWARA,T. HUNTER,S.W. ; CHO,S.; ASPINAL,G.O .; BRENNAN,P.J. Chemical synthesis and serology of dissacharides and trissacharides of phenolic glycolipid antigens from the Leprosy bacillus and preparations of a dissacharide protein conjugate for serodiagnosis of leprosy. Infection and Immunity, v. 43(10), p.245-252, 1984.
HARBOE,M.; CLOSS,º BJUNG, G.; KRONVALL,G.; AXELSEN,N.H.- Mycobacterium leprae specific antibodies detected by radio immunoassay. Scand. J. Immunol., v. 7, p. 11-20, 1978.
HUNTER,S.W.; BRENNAN,P. J. A novel Phenolic Glycolipid from Mycobacterium leprae. Possible involved in immunogenicity and pathogenicity.
J. Bacteriol., v. 147(3), p. 728-735,1981.
HUNTER,S.W. ; FUGIWARA,T. ; BRENNAN,P.J. Structure and antigenicity of the major specific glycolipid antigen of Mycobacterium leprae. The Journal of Biological Chemistry, v. 257(24), p. 15072-15078, 1982.
KRONVALL,G. - The potential of immunological tests as a tool in the epidemiology of leprosy. Lepr. Rev., v. 52 (suppl 1), p. 207-219, 1981.
LAFERTE,J.; ABREU,E.G.; ROBAINA,R.; VEREZA, V. Ultramicroelisa para la detección de anticuerpos IgM anti M. leprae. Rev. Inst. Med. Trop. São
Paulo, v. 33(6), p. 491-495, 1991.
LANCET ( Anonymous) - Serological tests for leprosy. The Lancet, v. 8, p. 533- 535, 1986. (Editorial)
MENZEL,S.; HARBOE,M.; BERGSVIK,H.; BRENNAN, P.J. - Antibodies to a synthetic analog of phenolic glycolipid-1 of M. leprae in the healthy house-hold
contacts of patients with leprosy. Int. J. Lepr Other Mycobact. Dis., v. 55, p. 617-625, 1987.
PAYNE, N.S.;DRAPER,P.;REES,R.J.W.- Serological activity of purified glycolipid from Mycobacterium leprae. Int. J. Lepr. Other Mycobact. Dis., v. 50(2), p. 220-221, 1982.
REPKA,J.C.D.;BRANA,L.R.;FABRICIO,L.H.; et al. Imu-nologia na hanseníase - Aspectos de avaliação imunológica no tratamento. Arq. Biol. Tecnol., v.
34(1), p. 53-60, 1991.
SAAD,M.H.F.;MEDEIROS,M.A.; GALLO, M.E.N.; et al. IgM immunoglobulins reacting with the phenolic glycolipid-1 antigen from M. leprae in sera of
leprosy patients and contacts. Mem. Inst. O. Cruz, v. 85(2):191-194,1990.
SOEBONO,H. & KLATSER,P.R. - A seroepidemiological study of leprosy in high and low endemic Indonesian villages. Int. J. Lepr. Other Mycobact.
Dis., v. 59, 416-425, 1991.
TOUW,J.; LANGNDIJK, E.M.J.; STONER, G.L.; BELIHU,A. Humoral immunity in leprosy: immunoglobulin G and M antibody responses to Mycobacterium leprae in relation to various disease patterns. Infect. Immunit., v. 36(3), p. 885-892, 1982.
YAMASHITA, J.T.; CRUAD, P.; ROTTA, O.; DAVID, L.H.; - Circulating immune complexes in leprosy sera: demonstration of antibodies against mycobacterial glycolipid antigens in isolated immune complexes. Int. J. Lepr. Other Mycobact. Dis., v. 61(1), p.44-50,1993.
YOUNG, D.B.; DESSANAYAKE, S.; MILLER, R.A.; KHANOLKAR, S.; BUCHANAN, T.M. - Humans respond predominantly with IgM immunoglobulin to the species-specific glycolipid of Mycobacterium leprae. The Journal of Infectious Diseases, v. 149(6), p. 870-874, 1984.
YOUNG, D.B.; HARNISH, I.; KNIGHT, J.; BUCHANAN, T.M.- Detection of phenolic glycolipid-1 in sera from patients with lepromatous leprosy. The Journal of Infectious diseases, v. 152 (5),p. 1078-1081, 1985.
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