Estudo da sensibilidade e especificidade do teste Elisa Anti PGL-1 no estado de São Paulo

Mitie Tada L.R.F. Brasil; Luiz Roberto de Oliveira; Carmem Silva de Mello; Paulo Mutuko Nakamura; Marli P. Manini; Denise teiner; Osmar Rotta

doi:10.47878/hi.1997.v22.36449

Authors

Mitie Tada L.R.F. Brasil Centro de Vigilância Epidemiológica Prof. A . Vranjac - SES-SP
Luiz Roberto de Oliveira Departamento de Saúde Pública- Faculdade de Medicina de Botucatu - UNESP
Carmem Silva de Mello Instituto Adolfo Lutz- SES-SP
Paulo Mutuko Nakamura Instituto Adolfo Lutz- SES-SP
Marli P. Manini Divisão de Dermatologia Sanitária - SES- SP
Denise teiner Serviço de Dermatologia da Faculdade de Medicina de Jundiaí - SP
Osmar Rotta Departamento de Dermatologia da Escola Paulista de Medicina - UNIFESP - SP

DOI:

https://doi.org/10.47878/hi.1997.v22.36449

Keywords:

Leprosy, ELISA, phenol-glyco-lipid - PGL-1

Abstract

Serum tests for leprosy diagnosis, using phenol-glyco-lipid -1 considered to be a specific antigen of the M. leprae, has brought about some possibilities to the study of the epidemiological behavior of the disease. A study was performed to evaluate the sensibility and specificity of an ELISA anti PGL-1 test using Cuban material and techniques (UMELISA). Eightyfour patients and a control group with 112 persons were tested.
The sensibility of the test was larger for the multibacilar patients with higher levels for the cases classified as Lepromatous (L) followed by the Bordeline (B) ones. In the multibacilar group, considering a 0.200 cut-off level, it was observed that only 75.0% of the Lepromatous patients
and 50.0% of the Bordeline patients tested positive. For a 0.300 cut-off level, only 64.3% of the L patients and 40.0% of the B patients still tested positive. The undetermined form patients presented a larger rate of positive results than the tuberculoide form and that may be a hint of a
polarization of some cases of the multibacilar forms. The specificity of the test was 87.5% at the 0.200 cut-off level and 99.1% at 0.300. This test, as some others, presented high specificity and low sensibility, resulting a large percentage of false negative tests. As leprosy is a disease with few serum tests, it is found that further technological development of this test should be stimulated, although its indiscriminate use for large scale
screening of the general population is not recommended. This way, advances in the research of more sensitive and specific tools for early diagnosis could be reached allowing for efficient intervention on the transmission chain of the disease.

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