Implantation of Autologous Keratinocytes in lower limb ulcers of patients with leprosy sequelae

Authors

  • Fátima Regina Vilani Moreno PhD, Pesquisadora Científica, Equipe Técnica de Biologia, Instituto Lauro de Souza Lima, Bauru, SP
  • Heloísa Cristina Quatrini Carvalho Passos Guimarães PhD, Pesquisadora Científica, Equipe Técnica de Clínica e Terapêutica, Instituto Lauro de Souza Lima, Bauru, SP
  • Sidinéia Raquel Bazalia Bassoli MSc, Enfermeira, Divisão de Enfermagem, Instituto Lauro de Souza Lima, Bauru, SP.
  • Roseli Marega Oda MSc, Enfermeira, Divisão de Enfermagem, Instituto Lauro de Souza Lima, Bauru, SP.
  • Érika Mozer BS, Biologista, Equipe Técnica de Imunologia, Instituto Lauro de Souza Lima, Bauru, SP.
  • Wladimir Fiori Bonilha Delanina MD, Médico Dermatologista, Divisão de Dermatologia, Instituto Lauro de Souza Lima, Bauru, SP.
  • Maria Ângela Bianconcini Trindade MD, PhD, Pesquisadora Científica, Instituto de Saúde e Divisão de Clínica Dermatológica, Hospital das Clínicas, Faculdade de Medicina-USP, São Paulo, SP.
  • Joel Carlos Lastória MD, PhD, Professor, Departamento de Dermatologia, Faculdade de Medicina de Botucatu, UNESP, Botucatu, SP
  • Norma Gondim Cleto MD, Médica Dermatologista, Divisão de Dermatologia, Instituto Lauro de Souza Lima, Bauru, SP. normagc@uol.com.br Correspondência para: Fátima Regina Vilani Moreno, Instituto Lauro de Souza Lima, Rod. Cmte. João Ribeiro de Barros, Km 225/226, 17034-971 Bauru, SP, Brasil.

DOI:

https://doi.org/10.47878/hi.2012.v37.35081

Keywords:

leprosy, keratinocytes, lower limb ulcers, fibrin glu

Abstract

The occurrence of ulcers in leprosy is mainly due to the existence of numb areas that can easily undergo trauma and burns and thereafter become secondarily infected. This study aimed to treat ulcers in lower limbs of patients with leprosy sequelae through the implantation of autologous keratinocytes. The study consisted of fourteen patients (six with a single ulcer, four with more than one ulcer in the same limb and four with ulcers in both limbs) with a total of 31 ulcers. A fragment of healthy skin was collected and subjected to enzymatic digestion to obtain keratinocytes, which were then cultured for four weeks. The resulting keratinocytes were implanted in ulcers in combination with fibrin glue. Patients were followed for two months and the ulcers were monitored weekly during this time. Ulcers measuring ≤ 9.0 cm high x 5.0 cm wide (23 ulcers) decreased by 68.8% ± 27.1%, with nine of them showing complete healing. Larger ulcers decreased by 50.0% ± 31.9%, with two of these healing completely. Thus, implantation of autologous keratinocytes in combination with fibrin glue was effective in healing and/or reducing the ulcer size and should be considered as an additional ulcer treatment option in patients with leprosy sequelae.

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References

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Published

2012-06-30

How to Cite

1.
Moreno FRV, Guimarães HCQCP, Bassoli SRB, Oda RM, Mozer Érika, Delanina WFB, Trindade M Ângela B, Lastória JC, Cleto NG. Implantation of Autologous Keratinocytes in lower limb ulcers of patients with leprosy sequelae. Hansen. Int. [Internet]. 2012 Jun. 30 [cited 2024 May 14];37(1):11-8. Available from: https://periodicos.saude.sp.gov.br/hansenologia/article/view/35081

Issue

Vol. 37 No. 1 (2012)

Section

Original articles

License

This journal is licensed under a Creative Commons Attribution 4.0 International License.

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