Avaliação da atividade macrofagica na hanseníase virchowiana e tuberculóide
DOI:
https://doi.org/10.47878/hi.1995.v20.35445Keywords:
Macrophages, leprosy, tumor necrosis factor aTNFAbstract
To verify the immunologic alternations related to the polar forms of leprosy the aTNF (tumor necrosis factor), levels was determined in vivo" and in vitro" in 31 individuals: lepromatous leprosy - 1, tuberculoid leprosy - 10 and normal controls - 10. PBMC (peripheral blood mononuclear cells) were cultured during 24 hs/379C/CO2 atmosphere and aTNF levels were easured by ELISA assay, at the cultures supernatants and the serum from the three groups of individuals. The results (pg/ml) showed TNF levels significantly higher in tuberculoids patients when compared with the levels of lepromatous leprosy (LL) or controls, indicating a deficit in development and maintenance of immunecellular response, favouring the infection dissemination in LL
patients. The negative correlation between aTNF levels and baciloscopic index suggest that elevated baciloscopic levels are associated with immunecelular supression.
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References
2. Barbieri TA & Correa WM: Human macrophage culture the leprosy prognostic test (LPT) Int. J. Lepr. 35: 377-380, 1967.
3. Beiguelman B & Barbieri TA: Comportamento dos macrófagos nasformas polares da lepra. Ciência e Cult. 17: 304-307, 1965.
4. Champsi J, Young LS and Bermudez LE: Production of TNFa, IL6 and TGF-13, and expression of receptors for TNFa and IL6, during
murine Mycobacterium avium infection. Immunology 84: 549-554, 1995.
5. Chang J, Xing Y, Megliozzo RS and Bloom BR: Killing of virulent Mycobacterium tuberculosis by reactive nitrogen intermediates
produce by active murine macrophages. J. Exp. Med. 176: 11111122, 1992.
6. Chonaib S, Branellec D and Burman WA: More insights into the complex physiology of TNF. Immunol. Today 12: 141-142, 1991.
7. Dinarello CA and Thompson RC: Blocking of IL1: IL1 receptor antagonist "in vivo" e "in vitro". Immunol. Today 12 404-410, 1991.
8. Foss NT, Oliveira EB, Silva CL: Correlation between TNF production increase of plasmaCreactive protein level and supression of T
lymphocyte response to concanavalin-A during erythema nodosum leprosum. Mt. J. Leprosy 61: 218-225, 1993.
9. Goulart IMB: Detecção de TGF131 em lesões cutâneas de diferentes formas clinicas de hanseníase. Dissertação de Mestrado - Universidade Federal de Uberlândia: 32-38, 1995.
10. Hirano T, Akira S, Taga T and Kishimoto T: Biological and Clinical aspects of Interleukin 6. Immunol. Today 11: 443-449, 1990.
11. Hsieh CS, Macatonia SE, Tripp CS, Wolf SF, Ogarro A and Murphy KM: Development of Th1 CR4+T cells through IL12 produced by Listeria-induced macrophages. Sciences 260: 547-549, 1993.
12. Kalaeb B, Kiessling R, Van Embden JDA, ThoIeJER, Kumararatne S, Pisa P, Wondimu A, Ottenhoff THM: Induction of antigen- specific CD4+ HLA-DR restricted cytotoxic T lymphocytes as well as nonspecific nonrestricted killer cells by the recombinant mycobacterial 65 Kda heat shok protein. Eur. J. Immunol. 20: 369-377, 1990.
13. Modlin RL, Kato H, Mehra V, Nelson EE, Xuedong F, Rea TH, Pattengalle PK and Bloom BR: Genetically restricted suppressor T-cell
clones derived from lepromatous leprosy lesions. Nature 322 459-461,1986.
14. Ottenhoff THM: Immunology of leprosy: lesions from and for leprosy.
15. Sarno EN, Grau GE, Vieira LM, Nery JA: Serum levels of tumor necrosis factor and interleukin 1 beta during leprosy reactional states. Clin. Exp. Immunol. 84: 103-108, 1991.
16. Silva CL, Foss NT: Tumor necrosis factor in leprosy patients. J. of infect Dis. 159:787790, 1989.
17. Silva CL, Faccioli LH, Foss NT: Supression of human monocyte cytokine release by phenolic glicolipid-1 of Mycobacterium leprae 61: 107-108, 1993.
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