Transformação dos linfócitos induzida pela fitohemaglutinina e reação de Mitsuda

Authors

  • J. A. VOZZA Laboratório de Patologia Clínica.
  • B. BEIGUELMAN Departamento de Genética Médica, Faculdade de Ciências Médicas, Universidade Estadual de Campinas, SP, Brasil.
  • R. C. B. PISANI Departamento de Genética Médica, Faculdade de Ciências Médicas, Universidade Estadual de Campinas, SP, Brasil.
  • P. C. CEZAR Laboratório de Patologia Clínica.

DOI:

https://doi.org/10.47878/hi.1977.v2.35650

Keywords:

Lymphocyte, Phytohemagglutinin, Mitsuda's reaction, Serum globulins

Abstract

The rate of lymphocyte transformation brought about by phytohemagglutinin as well as the serum globulins levels were investigated among healthy Brazilian caucasoid soldiers exhibiting either a strongly positive or a completely negative late lepromin reaction (Mitsuda reaction). None of them were contacts of leprosy patients. It is concluded that among healthy individuals non contacts of leprosy patients neither the lymphocyte transformation stimulated by phytohemagglutinin, nor the serum globulins levels depend upon the Mitsuda reaction. The significant negative correlation between the level of non gama globulins and the rate phytohemagglutinin induced lymphocyte transformation, which is found among lepromatous patients, could not be observed among the healthy individuals who were examined.

Downloads

Download data is not yet available.

References

BECHELLI, L. M.; GARCIA, G.; NAKAMURA, S.; QUAGLIATO, R. Determinação da data de leitura da reação de Mitsuda com lepromina integral em indivíduos sãos, sem exposição prévia conhecida ao M. leprae. In: CONG. INT. LEPROL., 8.°, Rio de Janeiro, 1963. Anais. v.
3, p. 284-294.
BEIGUELMAN, B. Lepromin reaction. Genetics studies including twin pair analysis. Acta Leprol., (44) : 5-65, 1971.
BEIGUELMAN, B. & PISAM, R C. B. Effect of DDS on phytohemagglutinin induced lymphocyte transformation. Int. J. Lepr., 42: 412-415, 1974.
BEIGUELMAN, B.; PINTO JR., W.; PISANI, R. C. B.; KRIEGER, H.; VOZZA, J. A.; EL-GUINDY,
M. M. Lymphocyte transformation and lepromatous leprosy. Ciência e Cultura, 27: 217-220, 1975.
BULLOCK, W. E. & FASAL, P. Studies of immune mechanism in leprosy: III — The role of cellular and humoral factors in impairment of the in vitro immune response. J. Immunol., 106: 888-899, 1971.
COOPERBRAND, S. R.; BONDEVICK, H.; SCHMID, K.; MANNICK, J. A. Transformation of human lymphocytes: inhibition by homologous alpha globulin. Science, 159: 1243-1244, 1968.
DIERKS, R. E. & SHEPARD, C. C. Effect of phytohemagglutinin and various mycobacterial antigens on lymphocyte cultures from leprosy patients. Proc. Soc. Exp. Biol. Med., 127: 391-395, 1968.
FLIESS, E. L.; BALINA, L. M.; BACHMANN, A. E.; CARDAMA, J. E.; GATTI, J. C. Alteraciones de la desdiferenciación linfoblástica en pacientes de lepra lepromatosa y sus consanguíneos sapos lepromino-negativos. Leprologia, 17: 83-90, 1972.
HAN, S. H.; WEISER, R. S.; LIN, Y. C. Transformation of leprous lymphocytes by lepromin tuberculin and phytohemagglutinin. Int. J. Lepr., 39: 789-795, 1971.
NELSON, D. S.; NELSON, M.; THURSTON, J. M.; WAITERS, M. F. R.; PEARSON, J. M. H. Phytohemagglutinin-induced lymphocyte transformation in leprosy. Clin. Exp. Immunol., 9: 33-43, 1967.
POTTER, J. C. Sur la transformation lymphoblastique des lymphocytes de lépreux provoquée par broyat de léprome murin. Bull. Soc. Pathol. Exot., 62: 987-992, 1969.
RODRIGUEZ-PARADISI, E.; BONAPARTE, Y. P.; MORGENFELD, M. C. Cultivo de linfócitos en enfermos con lepra lepromatosa. Leprologia. 12: 61-63, 1967.RODRIGUEZ-PARADISI, E.; BONAPARTE, Y. P.; MORGENFELD, M. C. Blasts in lepromatous leprosy. Lancet, 1: 308-309, 1968.
ROSENFELD, G Corante pancrômico para hematologia e citologia clínica. Nova contribuição para componentes do May-Grüwald e do Giemsa num só corante de emprego rápido. Mem. Inst. Butantan, 20: 329-334, 1947.
SHEAGREN, J. N.; BLOCK, J. B.; TRAUTMAN, J. R.; WOLF, S. M. Immunologic reactivity in patients with leprosy. Ann. Intern. Med., 70: 295-302, 1969.
ULRICH, M.; SALAS, B.; CONVIT, J Int. J. Lepr., 40: 4-9, 1972.
WONG, P. C.; CHAN-TEOH, C. H.; hemagglutinin in leprosy sera. Lymphocyte transformation with phytomitogens in leprosy.
KENDALL, F H Transformation of lymphocytes by phytoInt. J. Lepr., 39: 7-13, 1971.

Published

1977-06-30

How to Cite

1.
VOZZA JA, BEIGUELMAN B, PISANI RCB, CEZAR PC. Transformação dos linfócitos induzida pela fitohemaglutinina e reação de Mitsuda. Hansen. Int. [Internet]. 1977 Jun. 30 [cited 2024 May 15];2(1):53-9. Available from: https://periodicos.saude.sp.gov.br/hansenologia/article/view/35650

Issue

Section

Artigos de investigação científica