Keywords
Hanseniasis
Immunology
Rubino's reactio
Electrophoresi
Immunoelectrophoresis
Immunology
Rubino's reactio
Electrophoresi
Immunoelectrophoresis
How to Cite
1.
SILVA OP, FERRI RG, MORAES N, MARQUES ALV. I. Electrophoi?esis and immunoelectrophoresis of serum proteins. Tentative association to rubino’s reaction. Hansen. Int. [Internet]. 1976 Jun. 30 [cited 2024 Nov. 24];1(1):33-41. Available from: https://periodicos.saude.sp.gov.br/hansenologia/article/view/35673
Abstract
It was attempted to establish if leprosy patients with positive Rubino's reaction would display immunological characteristics that could distinguish them from those with negative Rubino's reaction. By electrophoresis, Rubino-negative patients (virchowian and tuberculoid) showed higher levels of alpha2, globulin than Rubino- positive patients Virchowian and normal individuals. Beta globulin levels were decreased in Rubino-positive and increased in Rubinonegative Virchowian patients. By immunoelectrophoresis similar results for the two lepromatous groups: constant increase of IgG znd IgM and unfrequent raise of lgA were found.
References
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10. HEREMANS, J. R. Immunochemical studies on protein pathology. The immunoglobulin concept. Clin. Chim. Acta, 4:639-646, 1959.
11. JARDIN, C. & BEYTOUT, D. Etude des protéines sériques du Soudanais hansénien par microélectrophorèse. Méd. Trop., 20:81-101, 1960.
12. KOHN, J. A cellulose acetate supporting medium for zone electrophoresis. Clin. Chim. Acta, 2:297-303, 1957.
13. KONG, M.; TSUGAMI, H.; SAKURAI, H. On serum protein components and leucocyte percentage of erythema nodosum leprosum. Lepro, 21:98, 1952.
14. LIM, S. D. & FUSARO, R. M. Leprosy. β2A and β2m immunoglobulins in leprosy sera. Arch. Derm., 89 (1) :86-88, 1964.
15. MASANTI, J. G. Proteínas séricas en la lepra. Modifications en las diferentes formas clínicas, en la amiloidosis y acción de los glucocorticóides. Leprologia, 4 (3) : 143-152, 1959.
16. MAUZE, J. & ARNAUD, G. L'electrophorèse du serum de lépreux. Int. J. Leprosy, 22(1) :55-60, 1954.
17. MORAN, C. J.; TURK, J. L.; RYDER, G.; WATERS, M. F. R. Evidence for circulating immune complexes in lepromatous leprosy. Lancet, 2:572-573, 1972.
18. MUELLING JR., R. J.; GOETZ, C.; ROSS, S. H. Serum protein patterns in leprosy. I. Carville survey. Int. J. Leprosy, 28 (2) :144-154, 1960.
19. NEILL, M. H. & DEWAR, M. M. The plasma proteins in leprosy. Publ. Health Bull., 168:1-20, 1927.
20. RUBINO, M. C. Nueva reacción serológica de la lepra. Rev. Méd. Urug., 29:144-151, 1926.
21. RUBINO, M. C. Séro-diagnostic de la lepre par l'agglutino-sédimentation des globules de mouton formolés. Ann. Inst. Pasteur, 47:147-172, 1931.
22. SEIBERT, F. B. & NELSON, J. W. Electrophoresis of serum. Serum proteins in tuberculosis and other chronic diseases. Amer. Rev. Tuberc., 47:66-77, 1943.
23. SERIE, C. & SCHALLER, K. F. L'électrophorèse et la lèpre. Bull. Soc. Path. Exot., 50(1) :17-21, 1957.
24. SIEGEL, S. Nonparametric statistics for the behavioral sciences. New York, McGrawHill, 1956. p. 116, 184.
25. TARABINI-CASTELLANI, G. Considerations del origen de la disproteinemia hanseniana y sus efectos. Rev. Leprol. Fontilles, 4(4) :227-234, 1957.
26. UYGUANCO, M. L. G.; RODRIGUES, J. N.; CONCEPCION, I. Studies on the biochemistry of leprosy. J. Pak. Med. Ass., 26:65-73, 1950.
27. VAZ, C. A. C.; FERRI, R. G.; GEISDHOVEL, N.; CAMPOS, A. N. P. Eletroforese sobre acetato de celulose (CAF). Reprodutibilidade e valores normais. Rev. Inst. Adolfo Lutz, 31:71-75, 1971.
2 ARU, L Il quadro sieroproteico nella lepra. Arch. Ital. Sei. Med. Trop. Parasit., 89(8):527-542, 1958.
3. BENHAMOU, E.; FERRAND, B.; SORREL, A.; CHICHE, J. C. Les controles biologiques du traitement des lépreux. Presse Méd., 64(76) :17291732, 1956.
4. BIER, O. & ARNOLD, K. Estudos sobre a sorologia da lepra. I. Sobre a especificidade e a sensibilidade da reação de Rubino. Pesquisas sobre o mecanismo da reação. Folia Clin. Biol., 7(1) :1-8, 1935.
5. BONOMO, L.; DAMMACCO, F.; GILLARDI, U. Hypergammaglobulinemia, secondary macroglobulinemia and paraproteinemia in leprosy. Int. J. Leprosy, 37(3): 280-287, 1969.
6. CURBAN, G. V. Contribuição para o estudo da reação de Rubino. Rev. Bras. Leprol., 30(4) :179-216, 1962.
7. FERRI, R. G. & COSSERMELLI, W. Analyse immuno-électrophorétique micro et macro méthodes. Rev. Franç. Étud. Clin. Biol., 9:134-138, 1964.
8. GORNALL, A. G.; BARDAWILL, C. J.; DAVID, M. M. J. Determination of serum proteins by means of the biuret reaction. J. Biol. Chem., 177:751766, 1949.
9. GRABAR, P. & WILLIAMS, C. A. Méthode permettant l'étude conjuguée des propriétés électrophorétiques et immunochimiques d'un mélange de protéines. Application au serum sanguin. Biochim. Biophys. Acta, 10:193-194, 1953.
10. HEREMANS, J. R. Immunochemical studies on protein pathology. The immunoglobulin concept. Clin. Chim. Acta, 4:639-646, 1959.
11. JARDIN, C. & BEYTOUT, D. Etude des protéines sériques du Soudanais hansénien par microélectrophorèse. Méd. Trop., 20:81-101, 1960.
12. KOHN, J. A cellulose acetate supporting medium for zone electrophoresis. Clin. Chim. Acta, 2:297-303, 1957.
13. KONG, M.; TSUGAMI, H.; SAKURAI, H. On serum protein components and leucocyte percentage of erythema nodosum leprosum. Lepro, 21:98, 1952.
14. LIM, S. D. & FUSARO, R. M. Leprosy. β2A and β2m immunoglobulins in leprosy sera. Arch. Derm., 89 (1) :86-88, 1964.
15. MASANTI, J. G. Proteínas séricas en la lepra. Modifications en las diferentes formas clínicas, en la amiloidosis y acción de los glucocorticóides. Leprologia, 4 (3) : 143-152, 1959.
16. MAUZE, J. & ARNAUD, G. L'electrophorèse du serum de lépreux. Int. J. Leprosy, 22(1) :55-60, 1954.
17. MORAN, C. J.; TURK, J. L.; RYDER, G.; WATERS, M. F. R. Evidence for circulating immune complexes in lepromatous leprosy. Lancet, 2:572-573, 1972.
18. MUELLING JR., R. J.; GOETZ, C.; ROSS, S. H. Serum protein patterns in leprosy. I. Carville survey. Int. J. Leprosy, 28 (2) :144-154, 1960.
19. NEILL, M. H. & DEWAR, M. M. The plasma proteins in leprosy. Publ. Health Bull., 168:1-20, 1927.
20. RUBINO, M. C. Nueva reacción serológica de la lepra. Rev. Méd. Urug., 29:144-151, 1926.
21. RUBINO, M. C. Séro-diagnostic de la lepre par l'agglutino-sédimentation des globules de mouton formolés. Ann. Inst. Pasteur, 47:147-172, 1931.
22. SEIBERT, F. B. & NELSON, J. W. Electrophoresis of serum. Serum proteins in tuberculosis and other chronic diseases. Amer. Rev. Tuberc., 47:66-77, 1943.
23. SERIE, C. & SCHALLER, K. F. L'électrophorèse et la lèpre. Bull. Soc. Path. Exot., 50(1) :17-21, 1957.
24. SIEGEL, S. Nonparametric statistics for the behavioral sciences. New York, McGrawHill, 1956. p. 116, 184.
25. TARABINI-CASTELLANI, G. Considerations del origen de la disproteinemia hanseniana y sus efectos. Rev. Leprol. Fontilles, 4(4) :227-234, 1957.
26. UYGUANCO, M. L. G.; RODRIGUES, J. N.; CONCEPCION, I. Studies on the biochemistry of leprosy. J. Pak. Med. Ass., 26:65-73, 1950.
27. VAZ, C. A. C.; FERRI, R. G.; GEISDHOVEL, N.; CAMPOS, A. N. P. Eletroforese sobre acetato de celulose (CAF). Reprodutibilidade e valores normais. Rev. Inst. Adolfo Lutz, 31:71-75, 1971.
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