Keywords
Mycobacterium leprae
Counterstain
Counterstain
How to Cite
1.
SIQUEIRA LF de G, ALMEIDA RG de, BELDA W. Modificação da coloração de fundo da técnica de Ziehl-Neelsen Na identificação do Mycobacterium leprae. Hansen. Int. [Internet]. 1982 Nov. 30 [cited 2024 Nov. 23];7(2):88-94. Available from: https://periodicos.saude.sp.gov.br/hansenologia/article/view/36129
Abstract
Authors analyse the counterstain with methylene blue solution in the usual Ziehl-Neelsen method. Considerations are made on the characteristics of the dye substance. A technique alteration is proposed: a "concomitant alkalization" of the classic aqueous methylene blue solution, by adding some drops of sodium hydroxide solution, 1:500, on the slide at the moment of the staining. By this tecnique it was observed: a) a larger validity period of the solution; b) an absence of precipitate in the slides; c) an easier visualization of the substrate; d) a larger contrast among the substrate and the bacilli.
References
1 BIER, O. Técnicas bacteriológicas. Método de Ziehl-Neelsen. In. — Bacteriologia e imunologia. 18.ed. São
Paulo, Melhoramentos, 1977. cap. 48, p.840.
2 BRASIL. Ministério da Saúde. Divisão Nacional de Pneumologia Sanitária. Coloração de Ziehl-Neelsen. In: —
Manual de bacteriologia da tuberculose. Rio de Janeiro, 1980. p.25-26.
3 COLOUR index. 3.ed. Yorkshire, Society of Dyers and Colourists, 1971.
4 FRANKEL, S. ; REITMAN, S. ; SONNENWIRTH, A.C., ed. Gradwohl's clinical laboratory methods and
diagnosis. 7.ed. Saint Louis, Mosby, 1970. v.1.
5 KANTOR, I.N. El examen microscópico. La coloración del extendido por el método de Ziehl-Neelsen. In: — Bacteriologia de la tuberculose humana y animal. Buenos Aires, OPS, 1979. p.22. (Série de monografias cientificas y técnicas. Centro Panamericano de Zoonosis, 11).
6 LILLIE, R.D. Acid fast stains. In: — Histopathological technic and practical histochemistry. 3.ed. London, McGraw Hill, 1965. p.575-581.
7 LIMA, A.O.; SOARES, J.B.; GRECO, J.B. ; GALIZZI , J. ; CANÇADO, J.R. Métodos de laboratório aplicados à clínica. 4. ed. Rio de Janeiro, Guanabara Koogan, 1969. 653p.
8 MALLORY, F.B. & WRIGHT, J.H. Pathological technique; a practical manual for workers in pathological histology and bacteriology. Philadelphia, W.B. Saunders, 1921. 555p.
9 PHARMACOPEIA dos Estados Unidos do Brasil. São Paulo, Editora Nacional, 1929.
10 THE PHARMACOPOEIA of the United States of America. 12. ed. Easton, PA., Mack Printing, 1942. 880p.
11 TREO, M.M. Bacteriologia. Técnicas de coloração de bacilos álcool-ácido-resistentes. In: — BRASIL. Serviço
Nacional de Lepra. Noções de leprologia. Rio de Janeiro, Ministério da Saúde, 1969. p.15-17.
12 VESTAL, A.L. Microscopy and staining. Ziehl-Neelsen (Z-N). In: — Procedures for the isolation and
identification of mycobacteria. Washington, D.C., U.S. Department of Health, Education, and Welfare, 1969. sect.2, p.27-32. (Public Health Service Publication n.1995).
13 WORLD HEALTH ORGANIZATION. Leprosy: examination for the bacillus in nodules and skin lesions. In:
— Manual of basic techniques for a health laboratory. Geneva, 1980. Part 2, cap. 33, p.259-263.
Paulo, Melhoramentos, 1977. cap. 48, p.840.
2 BRASIL. Ministério da Saúde. Divisão Nacional de Pneumologia Sanitária. Coloração de Ziehl-Neelsen. In: —
Manual de bacteriologia da tuberculose. Rio de Janeiro, 1980. p.25-26.
3 COLOUR index. 3.ed. Yorkshire, Society of Dyers and Colourists, 1971.
4 FRANKEL, S. ; REITMAN, S. ; SONNENWIRTH, A.C., ed. Gradwohl's clinical laboratory methods and
diagnosis. 7.ed. Saint Louis, Mosby, 1970. v.1.
5 KANTOR, I.N. El examen microscópico. La coloración del extendido por el método de Ziehl-Neelsen. In: — Bacteriologia de la tuberculose humana y animal. Buenos Aires, OPS, 1979. p.22. (Série de monografias cientificas y técnicas. Centro Panamericano de Zoonosis, 11).
6 LILLIE, R.D. Acid fast stains. In: — Histopathological technic and practical histochemistry. 3.ed. London, McGraw Hill, 1965. p.575-581.
7 LIMA, A.O.; SOARES, J.B.; GRECO, J.B. ; GALIZZI , J. ; CANÇADO, J.R. Métodos de laboratório aplicados à clínica. 4. ed. Rio de Janeiro, Guanabara Koogan, 1969. 653p.
8 MALLORY, F.B. & WRIGHT, J.H. Pathological technique; a practical manual for workers in pathological histology and bacteriology. Philadelphia, W.B. Saunders, 1921. 555p.
9 PHARMACOPEIA dos Estados Unidos do Brasil. São Paulo, Editora Nacional, 1929.
10 THE PHARMACOPOEIA of the United States of America. 12. ed. Easton, PA., Mack Printing, 1942. 880p.
11 TREO, M.M. Bacteriologia. Técnicas de coloração de bacilos álcool-ácido-resistentes. In: — BRASIL. Serviço
Nacional de Lepra. Noções de leprologia. Rio de Janeiro, Ministério da Saúde, 1969. p.15-17.
12 VESTAL, A.L. Microscopy and staining. Ziehl-Neelsen (Z-N). In: — Procedures for the isolation and
identification of mycobacteria. Washington, D.C., U.S. Department of Health, Education, and Welfare, 1969. sect.2, p.27-32. (Public Health Service Publication n.1995).
13 WORLD HEALTH ORGANIZATION. Leprosy: examination for the bacillus in nodules and skin lesions. In:
— Manual of basic techniques for a health laboratory. Geneva, 1980. Part 2, cap. 33, p.259-263.
This work is licensed under a Creative Commons Attribution 4.0 International License.
Downloads
Download data is not yet available.
