Keywords
Leprosy
NADH- reductase
Diaphorase
NADH-methemoglobin-reductase
NADH- reductase
Diaphorase
NADH-methemoglobin-reductase
How to Cite
1.
Dalpino D, Diltor LAM, Opromolla VA. Atividade da NADH-redutase de metemoglobina em hemolisado e membranas eritrocitárias de pacientes hansenianos sob tratamento sulfônico. Hansen. Int. [Internet]. 1998 Nov. 30 [cited 2024 Dec. 22];23(1/2):14-26. Available from: https://periodicos.saude.sp.gov.br/hansenologia/article/view/36461
Abstract
We measured in the blood samples of 72, adults leprosy patients, who were ingesting 100 mg of dapsone/day, the levels of erythrocytes, hemoglobin, methemoglobin, sulphone and reticulocytes. NADH-methemoglobin-reductase was measured both in the hemolysate and ghost cells. As a control, identical tests, except for sulphone measure, were applied to blood samples from 72 healthy individuals who did not ingest any oxidant drug. Significant statistical differences regarding the values in erythrocytes, methemoglobin, reticulocytes and activity of NADH- reductase in the erythrocytes ghosts were found among these two groups. The mean enzyme activity in the hemolysate of the leprosy patients and the
healthy individuals has not differed significantly when expressed in UI/g.Hemoglobin/l. The enzyme activity in erythrocytes ghosts from leprosy patients was significantly smaller then that in erythrocytes ghosts from healthy individuals. The mean level of methemoglobin in the leprosy patients was higher than in the control group, probably due to oxidizer action of sulphone. Tends in view we have found smaller levels of enzymatic activity in the erythrocytes membranes of the leprosy patients and coincident levels in the hemolysate compared to controls, we can affirm that probably this is due to an enzymatic displacement from the cellular membrane to the cytoplasm, with the objective of maintaining in the erythrocyte a constant hemoglobinic balance, in spite of the sulphone oxidizer action.
References
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9. CHOURY, D., LEROUX, A. , KAPLAN, J.C. Membrane-bound cytochrome b5 reductase (methemoglobin reductase) in human erythrocytes. J. Clin. Invest., v.67, p.149-51, 1981.
10. COHEN, G., HOCHSTEIN, P. Generation of hydrogen peroxide in erythrocytes by hemolytic agents. Biochemistry, v.3, p.895-900, 1964.
11. CONROY, J.M., BAKER, J.D., MARTIN, W.J. et al. Acquired methemoglobinemia from multiple oxidants. Soot. Med. J., v.86, p.1156-1159, 1993.
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14. EDELHOCH, H., HAYAISHI, O., TEPLY, L.J. The preparat ion and properties of a soluble diphosphopyridine nucleotide cythocrome c reductase. J. biol. chem , v.197, p. 97-104, 1952.
15. EVELIN, K.A., MALLOY, H.T. Microdetermination o f o x y h emo g l o b i n , me t h emo g l o b i n a n d sulfhemoglobin in a single sample of blood. J.
biol. chem., v.126, p. 655-662, 1938.
16. FEIG, S.A., NATHAN, D.G., GERALD, P.S. et al. Congenital methemoglobinemia- the result of age-dependent decay of methemoglobin reductase. Blood, v.39, p.407, 1972.
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25. KITAJIMA, S., YASUKOCHI Y., MINAKAMI, S. Purification and properties of human erythrocyte membrane NADH-cytochrome b5 reductase. Arch. Biochem. Biophys. v.210, p.330-9, 1981.
26. KUMA, F., ISHIZAWA, S., HIRAYAMA, K , NAKAJIMA, H. Studies on methemoglobin redutase. I. Comparative studies of diaphorases f rom normal and methemoglobinemic erythrocytes. J. Biol. Chem., v.247, p.550-5, 1972.
27. KUMA, F., PROUGH, R.A., MASTER, B. S. S. Studies on methemoglobin reductase. Immunochemical similarity of soluble methemoglobin reductase and cytochrome b5 of human erythrocytes with NADH-citochrome b5 reductase and cytochrome b5 of rat liver microsomes. Arch. Biochem. Biophys. v.172, p.600-7, 1976,
28. LEROUX, A., TORLINSKI, L., KAPLAN, J.C. Soluble and microsomal forms of NADH-cytochrome b5 reductase from human placenta: Similarity with
NADH-methemoglobin reductase from human erythrocytes. Biochim. Biophys. Acta, v.481, p.50, 1977.
29. MAGNA L. A. , BEIGUELMAN, B. NADHMethemoglobin reductase and methemoglobinemia among leprosy patients. Int. J. Lepr.. v.52, p.475-81, 1984
30. MATSUKI, T., TAMURA, M., TAKESHITA, M. et al. Age-dependent decay of cytochrome b5 and cytochrome b5 reduc tase in human erythrocytes. Biochem. J., v.194, p.327-30, 1981.
31. MELDOLESI J., CORTE, G., PIETRINI, G. et al. Localization and biosynthesis of NADHcytochrome b5 reductase, an integral membrane protein, in rat liver cells. J. Cell Biol., v.85, p.516-26, 1980.
32. MIHARA, K., SATO, R., SAKAKIBARA, R. et al. Reduced nicotinamide adenine dinucleotide - cytochrome b5 reductase: Location of the hydrophobic, membrane-binding region at the carboxyl-terminal end and the masked amino terminus. Biochemistry, v.17, p.2829-34, 1978.
33. MILLER, A., SMITH, H.C. The intracellular and membrane effects of oxidant agents on normal red cells. Brit. J. Haem., v.19, p.417-28, 1970.
34. MILLS, G.C., RANDALL, H.P. - Hemoglobin catabolism II. The protection of hemoglobin from oxidative breakdown in the intact erythrocyte. J. Biol. Chem., v.232, p.589- 98, 1958.
35. NORDEEN, S.K., LOPES BRAVO, L., SUNDARESAN, T.K. Mise au point - nomble estimatif de cas de lepre dans le monde. Acta Lepr., v.8, p.121-5, 1993.
36. ORGANIZACION MUNDIAL DE LA SALUD. Una guia para el control de la lepra. Madri: Ministerio de Sanidad Y Consumo, 1988..
37. PANIN, G., PERNECHELE, M., GIURIOLI, R. et al. Cytochrome b5 reductase ac t ivi ty in erythrocytes and leukocytes as related to sex and age. Clin. Chem., v.30, p.701-3, 1984.
38. PASSON, P.G., HULTQUIST, D.E. Soluble cytochrome b5 reductase from human erythrocytes. Bioch. Biophys. Acta, v.275, p.62-73, 1972.
39. PETRAGNANI, N., NOGUEIRA, O.C., RAW, I. Methemoglobin reduction through cytochrome b5 reductase. Nature, v.184, p.1651. 1959.
40. RAPAPORT, S.I. Hemoglobinopatias e síndromes talassêmicas. In : ______ Introdução à hematologia. Rio de Janeiro: Harper & Row do Brasil. 1978. p.54-70.
41. Metemoglobinemia . In:________________ Introdução à hematologia. Rio de Janeiro: Harper & Row do Brasil,1978. p.109-10.
42. RASBRIDGE, M.R., SCOTT, G.L. The haemolytic action of dapsone : he efect on red-cell glycolysis . Brit. J. Haemat., v.24, p.169-81, 1973.
43. ROSS, J.D. Deficient activity of DPNH-dependent methemoglobin diaphorase in cord blood erythrocytes. Blood, v.21, p.51-62,1963
44. SCHWARTZ, J.M., JAFFÉ, E. R.Hereditary methemoglobinemia with deficiency of NADHdehydrogenase. In: STANBURY, J.B., WYNGAARDEN, J.B., FREDRICSON, D.S. eds. The metabolic basis of inherited disease. 4. ed.New York: McGraw-Hill, 1978. p.1452-64
45. SCHWARTZ, J.M., PARESS. P.S., ROSS, J.M., DIPILLO, F., RIZEK R. Unstable variant of NADH-methemoglobin reductase in Puerto Ricans with hereditary methemoglobinemia. J. Clin. Invest., v. 51, p.1594-601, 1972.
46. SCHWARTZ, J.M., REISS, A.L., JAFFÉ, E.R. Hereditary methemoglobinemia with deficiency of NADH-cytochrome b5 reductase. In: STANBURY, J.B., WYNGAARDEN, FREDRICSON, D.S., eds. The metabolic basis of inherited disease. New York: McGraw-Hill, 1983. p.1654-65.
47. SCOTT, E.M. The relation of diaphorase of humam erythrocytes to inheritance of methemoglobinemia. I. Clin. Invest., v.39, p.1176-9, 1960.
48. SCOTT, E. M., DUNCAN, I.W. , EKSTRAND, V. T h e reduced pyridine nucleotide dehydrogenases of human erythrocytes. J.Biol. Chem.,v.240, p.481-5, 1965.
49. SCOTT, E.M., GRIFFITH, I.V. The enzymic defect of hereditary methemoglobinemia- diaphorase (Preliminary notes). Biochem. and Biophys. Acta, v.34, p.584-6, 1959.
50. SCOTT, E.M., MCGRAW, J.C. Purification and properties of diphosphopyridine nucleotide diaphorase of human erythrocytes. J. Biol. Chem., v.237, p.249-52, 1962.
51. SIMPSON, I. A. Appendix. Method of sulfone estimations. Int. I. of Leprosy, v.17, p.208-10, 1949.
52. SOCIEDADE BRASILEIRA DE HEMATOLOGIA E HEMOTERAPIA. Enzimopatias eritrocitárias. In _______Manual de Técnicas e Recomendações
Hematológicas. São Paulo: 1975. p.125-23.
53. SPATZ, L., STRITTMATTER, P. A form of reduced nicotinamide adenine dinucleotide-cytochrome b5 reductase containing both the catalytic site
and an additional hydrophobic membranebinding segment. J. Biol. Chem. v.248, p.793-9, 1973.
54. SUGITA, Y., NOMURA, S., YONEYAMA, Y. Purification of reduced pyridine nucleotide dehydrogenase from human erythrocytes and methemoglobin reduction by the enzyme. I. BioL Chem., v.246, p.6072-78. 1971.
2. BALAKRISHNAN, S., KARTHIKEYAN, S., RAMU, G. Investigations into haemolytic effects of dapsone therapy in leprosy patients. Indian J. Med., v.61,
p.10-16, 1989.
3. BANZATO, C.E.M., MAGNA L.A. In vitro effect of Dapsone on NADH-methemoglobin reductase. Int. J. Leprosy, v.59, p.486-487, 1991.
4. BERNARD, J., et al. Anatomia e fisiologia do eritrócito e da série eritroblástica. In: ______ Manual de hematologia. Santos, 1989. p.15-35.
5. BEUTLER, E., BALUDA, M.C. Metemoglobin reduction. Studies of the interaction between cell populations and of the role of methylene blue. Blood, v.22, p. 323-33, 1963.
6. BUNN, H. F. Distúrbios da hemoglobina - metemoglobinemia. In: HARRISSON, T.R. Medicina interna. 13. ed. São Paulo: Mc Graw Hill, 1995. v.2., p.1823-1824.
7. CAMPBELL, J.M. , CAMPBELL, J.B. Cálculo baseado no coeficiente de extinção molar de um produto de reação com absorbânc ia. In: ______ Matemática de laboratório -cálculo de enzimas aplicações médicas e biológicas. 3.ed. São Paulo: Roca, 1986. p.214.
8 . CATICHA-ALFONSO, O.S., MAGNA, L.A., BEIGUELMAN, B. NADH - r e d u t a s e d e metemoglobina e reticulocitose. Ciência e Cultura, v.37, p.280-283,1985.
9. CHOURY, D., LEROUX, A. , KAPLAN, J.C. Membrane-bound cytochrome b5 reductase (methemoglobin reductase) in human erythrocytes. J. Clin. Invest., v.67, p.149-51, 1981.
10. COHEN, G., HOCHSTEIN, P. Generation of hydrogen peroxide in erythrocytes by hemolytic agents. Biochemistry, v.3, p.895-900, 1964.
11. CONROY, J.M., BAKER, J.D., MARTIN, W.J. et al. Acquired methemoglobinemia from multiple oxidants. Soot. Med. J., v.86, p.1156-1159, 1993.
12. CREAM, J. J., SCOTT, G.L. Anaemia in dermatitis herpetiformis. The role of Dapsone- induced haemolysis and malabsorption. Brit. J. Derm., v.82, p.333-342, 1970.
13. DODGE, J.T., MITCHELL, C., HANAHAN, D.J. The preparat ion and character ist ics of hemoglobin-free ghost of human erythrocytes. Arch. Biochem. Biophys., v.100, p.119-130, 1963.
14. EDELHOCH, H., HAYAISHI, O., TEPLY, L.J. The preparat ion and properties of a soluble diphosphopyridine nucleotide cythocrome c reductase. J. biol. chem , v.197, p. 97-104, 1952.
15. EVELIN, K.A., MALLOY, H.T. Microdetermination o f o x y h emo g l o b i n , me t h emo g l o b i n a n d sulfhemoglobin in a single sample of blood. J.
biol. chem., v.126, p. 655-662, 1938.
16. FEIG, S.A., NATHAN, D.G., GERALD, P.S. et al. Congenital methemoglobinemia- the result of age-dependent decay of methemoglobin reductase. Blood, v.39, p.407, 1972.
17. FINCH, C. A. Me t h emo g l o b i n emi a a n d sulfhemoglobinemia. The New Engl. J. of Med.v. 239, p.470-478, 1948.
18. HARLEY, J.D. , MAUER, A.M. Studies on the formation of Heins bodies. I. Methemoglobin production and oxyhemoglobin distruction. Blood, v.16, p.1722-35 , 1960.
19. HARLEY, J.D., MAUER, A.M. Studies on the formation of Heins bodies. II. The nature and significance of heins bodies. Blood, v.17, p.418-33, 1961.
20. HEGESH, E., CALMANOVICI, N., AVRON, M. - New method for determining ferrihemoglobin reductase (NADH-Methemoglobin Reductase) in
erythrocytes. J. Lab & Clin. Med.. v. 72, p.339-44, 1968.
21. HENRY, J.B. Hemoglobina. In: TODD, SANFORD, DAVIDSON. Diagnósticos clínicos e conduta terapêutica por exames laboratoriais. 16. ed., São Paulo: Manole, 1982. V.I, p. 946-54.
22. HOFFBRAND, A.V., PETTIT, J.E. Hematologia clínica ilustrada: manual e atlas colorido. São Paulo: Manole, 1991. p.4-7.
23. HUENNEKENS, F.M., CAFFREY, R.W., BASFORD, R.E., et al. Erythrocyte metabolism. IV. Isolation and properties of methemoglobin reductase. J. Biol. Chem., v.227, p.261-72, 1957.
24. JANDL, J.H., ENGLE, L.K., ALLEN, D.W. Oxidative hemolysis and precipitation of hemoglobin. I. Heinz body anemias as an acceleration of red cell aging. J. Clin. Invest., v.39, p.1818-36, 1960.
25. KITAJIMA, S., YASUKOCHI Y., MINAKAMI, S. Purification and properties of human erythrocyte membrane NADH-cytochrome b5 reductase. Arch. Biochem. Biophys. v.210, p.330-9, 1981.
26. KUMA, F., ISHIZAWA, S., HIRAYAMA, K , NAKAJIMA, H. Studies on methemoglobin redutase. I. Comparative studies of diaphorases f rom normal and methemoglobinemic erythrocytes. J. Biol. Chem., v.247, p.550-5, 1972.
27. KUMA, F., PROUGH, R.A., MASTER, B. S. S. Studies on methemoglobin reductase. Immunochemical similarity of soluble methemoglobin reductase and cytochrome b5 of human erythrocytes with NADH-citochrome b5 reductase and cytochrome b5 of rat liver microsomes. Arch. Biochem. Biophys. v.172, p.600-7, 1976,
28. LEROUX, A., TORLINSKI, L., KAPLAN, J.C. Soluble and microsomal forms of NADH-cytochrome b5 reductase from human placenta: Similarity with
NADH-methemoglobin reductase from human erythrocytes. Biochim. Biophys. Acta, v.481, p.50, 1977.
29. MAGNA L. A. , BEIGUELMAN, B. NADHMethemoglobin reductase and methemoglobinemia among leprosy patients. Int. J. Lepr.. v.52, p.475-81, 1984
30. MATSUKI, T., TAMURA, M., TAKESHITA, M. et al. Age-dependent decay of cytochrome b5 and cytochrome b5 reduc tase in human erythrocytes. Biochem. J., v.194, p.327-30, 1981.
31. MELDOLESI J., CORTE, G., PIETRINI, G. et al. Localization and biosynthesis of NADHcytochrome b5 reductase, an integral membrane protein, in rat liver cells. J. Cell Biol., v.85, p.516-26, 1980.
32. MIHARA, K., SATO, R., SAKAKIBARA, R. et al. Reduced nicotinamide adenine dinucleotide - cytochrome b5 reductase: Location of the hydrophobic, membrane-binding region at the carboxyl-terminal end and the masked amino terminus. Biochemistry, v.17, p.2829-34, 1978.
33. MILLER, A., SMITH, H.C. The intracellular and membrane effects of oxidant agents on normal red cells. Brit. J. Haem., v.19, p.417-28, 1970.
34. MILLS, G.C., RANDALL, H.P. - Hemoglobin catabolism II. The protection of hemoglobin from oxidative breakdown in the intact erythrocyte. J. Biol. Chem., v.232, p.589- 98, 1958.
35. NORDEEN, S.K., LOPES BRAVO, L., SUNDARESAN, T.K. Mise au point - nomble estimatif de cas de lepre dans le monde. Acta Lepr., v.8, p.121-5, 1993.
36. ORGANIZACION MUNDIAL DE LA SALUD. Una guia para el control de la lepra. Madri: Ministerio de Sanidad Y Consumo, 1988..
37. PANIN, G., PERNECHELE, M., GIURIOLI, R. et al. Cytochrome b5 reductase ac t ivi ty in erythrocytes and leukocytes as related to sex and age. Clin. Chem., v.30, p.701-3, 1984.
38. PASSON, P.G., HULTQUIST, D.E. Soluble cytochrome b5 reductase from human erythrocytes. Bioch. Biophys. Acta, v.275, p.62-73, 1972.
39. PETRAGNANI, N., NOGUEIRA, O.C., RAW, I. Methemoglobin reduction through cytochrome b5 reductase. Nature, v.184, p.1651. 1959.
40. RAPAPORT, S.I. Hemoglobinopatias e síndromes talassêmicas. In : ______ Introdução à hematologia. Rio de Janeiro: Harper & Row do Brasil. 1978. p.54-70.
41. Metemoglobinemia . In:________________ Introdução à hematologia. Rio de Janeiro: Harper & Row do Brasil,1978. p.109-10.
42. RASBRIDGE, M.R., SCOTT, G.L. The haemolytic action of dapsone : he efect on red-cell glycolysis . Brit. J. Haemat., v.24, p.169-81, 1973.
43. ROSS, J.D. Deficient activity of DPNH-dependent methemoglobin diaphorase in cord blood erythrocytes. Blood, v.21, p.51-62,1963
44. SCHWARTZ, J.M., JAFFÉ, E. R.Hereditary methemoglobinemia with deficiency of NADHdehydrogenase. In: STANBURY, J.B., WYNGAARDEN, J.B., FREDRICSON, D.S. eds. The metabolic basis of inherited disease. 4. ed.New York: McGraw-Hill, 1978. p.1452-64
45. SCHWARTZ, J.M., PARESS. P.S., ROSS, J.M., DIPILLO, F., RIZEK R. Unstable variant of NADH-methemoglobin reductase in Puerto Ricans with hereditary methemoglobinemia. J. Clin. Invest., v. 51, p.1594-601, 1972.
46. SCHWARTZ, J.M., REISS, A.L., JAFFÉ, E.R. Hereditary methemoglobinemia with deficiency of NADH-cytochrome b5 reductase. In: STANBURY, J.B., WYNGAARDEN, FREDRICSON, D.S., eds. The metabolic basis of inherited disease. New York: McGraw-Hill, 1983. p.1654-65.
47. SCOTT, E.M. The relation of diaphorase of humam erythrocytes to inheritance of methemoglobinemia. I. Clin. Invest., v.39, p.1176-9, 1960.
48. SCOTT, E. M., DUNCAN, I.W. , EKSTRAND, V. T h e reduced pyridine nucleotide dehydrogenases of human erythrocytes. J.Biol. Chem.,v.240, p.481-5, 1965.
49. SCOTT, E.M., GRIFFITH, I.V. The enzymic defect of hereditary methemoglobinemia- diaphorase (Preliminary notes). Biochem. and Biophys. Acta, v.34, p.584-6, 1959.
50. SCOTT, E.M., MCGRAW, J.C. Purification and properties of diphosphopyridine nucleotide diaphorase of human erythrocytes. J. Biol. Chem., v.237, p.249-52, 1962.
51. SIMPSON, I. A. Appendix. Method of sulfone estimations. Int. I. of Leprosy, v.17, p.208-10, 1949.
52. SOCIEDADE BRASILEIRA DE HEMATOLOGIA E HEMOTERAPIA. Enzimopatias eritrocitárias. In _______Manual de Técnicas e Recomendações
Hematológicas. São Paulo: 1975. p.125-23.
53. SPATZ, L., STRITTMATTER, P. A form of reduced nicotinamide adenine dinucleotide-cytochrome b5 reductase containing both the catalytic site
and an additional hydrophobic membranebinding segment. J. Biol. Chem. v.248, p.793-9, 1973.
54. SUGITA, Y., NOMURA, S., YONEYAMA, Y. Purification of reduced pyridine nucleotide dehydrogenase from human erythrocytes and methemoglobin reduction by the enzyme. I. BioL Chem., v.246, p.6072-78. 1971.
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