In vitro effect of DDS on phytohaemagglutinin (PHA) induced lymphocyte transformation

S.K. Ghei; U. Sengupta; K.V. Desikan

doi:10.47878/hi.1980.v5.36105

Autores/as

S.K. Ghei Research Officer, Immunology Laboratory, Central JALMA Institute for Leprosy, Taj Ganj, Agra — 282 001, India.
U. Sengupta Senior Research Officer, Immunology Laboratory, Central JALMA Institute for Leprosy, Taj Ganj, Agra — 282 001, India.
K.V. Desikan Director, Central JALMA Institute for Leprosy, Tal Ganj, Agra — 282 001, Índia.

DOI:

https://doi.org/10.47878/hi.1980.v5.36105

Palabras clave:

Diamino diphenyl sulphone, Phytohaemagglutinin, Lymphocyte transformation test

Resumen

A study to find out the in vitro effect of diamino diphenyl sulphone (DDS) on phytohaemagglutinin (PHA) induced lymphocyte transformation was carried out in three phases using a wide range of DDS concentrations. Lymphocytes from healthy volunteers were investigated. Volunteers were divided into three groups to conduct the study in three phases. In each phase in addition to 0.02 ml of PHA, 4 different concentrations of DDS were added per 106 lymphocytes in tissue culture system. A statistically significant depression (P<0.05) in the per cent of blast cell formation induced by PHA was observed in the cultures with all the concentrations of DDS except with the lowest concentration (0.01 μg) of DDS. While the depression observed in the first and the third phase was found to be dose dependent, no significant correlation was noted between the DDS concentration and depression in PHA induced blastogenesis in the second phase. The significance of these observations are discussed.

Descargas

Los datos de descargas todavía no están disponibles.

Citas

BEIGUELMAN, B. & PISANI, R.C.B. 1974. Effect of DDS on phytohemagglutinin-induced lymphocyte transformation. Int. J. Lepr., 42(4) :412-415.
BOYUM, A. 1968. Separation of leukocytes from blood and bone marrow. Scand. J. Clin. Lab. Invest., 21 (Suppl. 97) :7.
LAMVIK, J.O. 1968. A microincubation chamber technic for testing the immunological activity of cultured lymphocytes. Scand. J. Haematol., 5:278-286.
RAMU, G.; SENGUPTA, U.; DESIKAN, K.V. 1980. Influence of sulphone therapy on lepromin reaction. Lepr. Rev., 51(3) :207-214.
SENGUPTA, U.; GHEI, S.K.; VENKATESAN, K.; BHARADWAJ, V.P. 1979. In vivo effect of DDS on phytohemagglutinin (PHA)-induced lymphocyte transformation cultures in normal healthy volunteers. Int. J. Lepr., 47(2) :167-170.