Abstract
Hydrosoluble vitamins B1, B2, B6 and PP are essential organic substances for human organism, functioning as coenzymes on several metabolic cycles. In the present investigation four vitamins of B complex and its vitamers contents were determined in royal jelly samples marketed in São Paulo, Brazil. A single extraction process was employed, and each vitamin was determined by HPLC using C18 column and detected by fluorescence. Four samples from different suppliers were analyzed, and the results varied from 0.08 to 0.41 mg/100g (vitamin B1 or thiamine); from 0.01 to 0.05 mg/100g (vitamin B2 or riboflavin); from 0.13 to 0.38 mg/100g (piridoxal - vitaminB6); from 0.26 to 1.38 mg/100g (piridoxamine - vitamin B6); from 0.21 to 0.57 mg/100g (niacin - vitamin PP);and from 1.56 to 2.00 mg/100g (niacinamide - vitamin PP). These data show that royal jelly is not an important source of the analyzed vitamins, though the results indicate that the employed technique is suitable for determining these four vitamins and its vitamers.
References
1. Bobbio F, Bobbio P. Introdução à química de alimentos. 2.ed. Varela (São Paulo): 1989.
2. Ball G. Bioavailability and analysis of vitamins in foods. 1.ed. Chapman & Hall (London): 1998.
3. Cooperman, JM, Lopez R. Ribofl avin. In: Machlin, J. Handbook of Vitamins. 2.ed. New York: M. Dekker; 1990. p. 299-328
4. Bianchini-Pontuschka R, Penteado MVC. Vitamina B2. In: Penteado, MVC. Vitaminas: aspectos nutricionais, bioquímicos, clínicos e analíticos. 1 ed. Barueri: Manole, 2003, p.279-316.
5. Driskell JA. Vitamin B6. In: Machlin, J. Handbook of Vitamins. 2.ed. New York: M.Dekker, 1990. p.379-402.
6. Hankes LV. Nicotinic Acid and Nicotinamide. In: Machlin, J. Handbook of Vitamins. 2.ed. New York: M. Dekker, 1990. p. 329-78.
7. Ottaway PB. The technology of vitamins in food, 1.ed. Blackie Academic & Professional (London): 1993.
8. Bianchini-Pontuschka R, Penteado MVC. Vitamina B6. In: Penteado, M.V.C. Vitaminas: aspectos nutricionais, bioquímicos, clínicos e analíticos. 1 ed. Barueri: Manole, 2003. p.367-96.
9. Sant’Ana HMP. Niacina. In: Penteado, M.V.C. Vitaminas: aspectos nutricionais, bioquímicos, clínicos e analíticos. 1 ed. Barueri: Manole, 2003. p.331-59.
10. Presoto A, Rios M, Almeida-Muradian LB. Simultaneous High Performance Liquid Chromatographic Analysis of Vitamins B1, B2 and B6 in Royal Jelly. J Braz Chem Soc. 2004; 15:136.
11. Moreschi ECP. Desenvolvimento e validação de métodos cromatográficos e avaliação da estabilidade de vitaminas hidrossolúveis em alimentos [Tese de Doutorado], Universidade de São Paulo, Brasil, 2006. 214 pp.
12. Lahély S, Bergaentzlé M, Hasselmann C. Fluorimetric determination of niacin in foods by high-performance liquid chromatography with post-column derivatization. Food Chemistry. 1999; 65:129.
13. Brasil. Ministério da saúde. Agência Nacional de Vigilância Sanitária [ANVISA]. Resolução RDC nº 269 de 22 de setembro de 2005. Aprova regulamento técnico sobre a ingestão diária recomendada (IDR) de proteína, vitaminas e minerais. Diário Oficial [da] República Federativa do Brasil, Brasília, DF.
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