Determinação espectrofotométrica do sulfato de neomicina em medicamentos, diretamente e após separação por cromatografia em camada delgada

Waldomiro Pregnolatto; Myrna Sabino

doi:10.53393/rial.1974.34.37056

Vol. 34 No. 1-2 (1974), ORIGINAL ARTICLE

Vol. 34 No. 1-2 (1974)

Spectrophotometric determination of neomycin sulfate in pharmaceutical products by thin-layer chromatography

ORIGINAL ARTICLE

https://doi.org/10.53393/rial.1974.34.37056

Published 1974-12-30

Waldomiro Pregnolatto⁺⁻
Myrna Sabino⁺⁻

Waldomiro Pregnolatto

Instituto Adolfo Lutz

Myrna Sabino

Instituto Adolfo Lutz

pdf (Português (Brasil))

Keywords

neomycin sulfate, determination in pharmaceuticals
thin-layer chromatography and spectrophotometry to determine neomycin

How to Cite

1.

Pregnolatto W, Sabino M. Spectrophotometric determination of neomycin sulfate in pharmaceutical products by thin-layer chromatography. Rev Inst Adolfo Lutz [Internet]. 1974 Dec. 30 [cited 2024 Jul. 22];34(1-2):41-6. Available from: https://periodicos.saude.sp.gov.br/RIAL/article/view/37056

Abstract

The contents of neomycin in a gíven pharmaceutical product can be promptly verified through the reaction of neomycin with ninhidryn in glycerinic medium and under certain pre-established conditions. The method herein described is based on the reaction of neomycín with the ninhidryn reagent containing glycerin. Under such conditions the reaction becomes more perceptible and its results reproducible. Beer law applíes to this case in a range between 4 to 40 flg neomycin sulfate per ml. Some interfering agents were studied as well. In cases where the neomycin was to be separated prior to the reaction, a thín-layer chromatography method was used.

https://doi.org/10.53393/rial.1974.34.37056

pdf (Português (Brasil))

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