Palavras-chave
hanseníase
estículos
ratos
ofloxacina
volume nuclear
cariometria
estículos
ratos
ofloxacina
volume nuclear
cariometria
Como Citar
1.
Paschoal VDA, Rossit AB, Bizutti LO, Azoubel R. Volume dos núcleos das células do testículo de ratos sob efeitos da ofloxacina. Hansen. Int. [Internet]. 30º de junho de 2005 [citado 12º de novembro de 2024];30(1):3-8. Disponível em: https://periodicos.saude.sp.gov.br/hansenologia/article/view/35487
Resumo
A ofloxacina possui amplo espectro de ação antimicrobiana, inclusive no combate ao Mycobacterium leprae, sendo atualmente empregada em substituição, quando da impossibilidade do uso da rifampicina. O objetivo deste foi estudar alterações nos núcleos das células do testículo de ratos púberes e respectivos grupos controle, submetidos à aplicação oral de ofloxacina. 0 método utilizado foi a morfometria, pela técnica cariométrica. As principais estruturas observadas nas preparações histológicas dos testículos foram: as células de Leydig, as espermatogônias e as células de Sertoli. Foram utilizados 09 ratos (Wistar), cinco tratados e quatro controles, aos quais foram administrados diariamente 12 mg/Kg/peso de ofloxacina, do 34Q ao 48Q dia após o nascimento. No 49a dia de vida, os ratos foram sacrificados. O estudo cariométrico das células de Leydig, das espermatogônias e das células de Sertoli de testículos dos animais tratados não evidenciou mudanças significativas na forma de seus núcleos (p > 0,05), quando comparados aos controles. Conclui-se que a oflocaxina na dose e período empregados não promoveu alterações nos constituintes celulares de testículos de ratos púberes.
Referências
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levofloxacin in rats and rabbits. Arzneimittelforschung 1992; 43(3A):374-7.
2 Abd-Allah AR, Aíy HA, Moustafa AM, Abdel-Aziz AA, Hamada FM, Adverse testicular effects of some quinolone members in rats.
Pharmacol Res 2000; 41(2);211-219.
3 Fleury RN. Comprometimento visceral na hanseníase. In; Noções de Hansenologia. Bauru: Hospital Lauro de Souza Lima. Centro
de Estudos Dr. Reynaldo Quagliato 1981. p.47-53.
4 Sapota L, Veksel A. Androgenic status in patients with lepromatous leprosy. Br) Urol 1994; 74(2):221-4.
5 Brady TM, Larner J, Minneman KP, Neu HC. Farmacologia humana: da molécula à clínica, 2ed, Rio de )aneiro: Guanabara Koogan; 1 997,
6 Paschoal VDA. Efeitos da ofloxacina no desenvolvimento dos testículos de ratos: estudo cariométricoo. [tese] São José do Rio Preto: Faculdade de Medicina de São José do Rio Preto; 2002.
7 Sala MAM, Matheus M, Valer! VA. A new stereological method for estimation: the thickness of a cellular layet on random sections.Mikroskopile Wien 1981; 38(516):I27-130.
8 Miraglia SM, Hayashí H, Goldfeder EM. Histomorfometria dos testículos de ratos albinos normais em várias idades. Rev bras ciênc morfol 1990; 7(1):8-15.
9 Santos HSL, Azoubel R. Embriologia comparada: texto e atlas, laboticabal: FUNEP; 1996.
10 Sadler TW. Langman/Embriologia médica. 7ed. Rio de Janeiro: Guanabara Koogan; 1997.
11 Zanato VF, Martins MP, Anselmo-Franci )A, Petenusci SO, Carvalho-Lamano TL. Sexual development of male Wistar rats. Braz j med res 1994; 27:1273-1280.
12 Rosa e Silva AAM, Guimarães MA, Lamano-Carvalho TL, Kempinas WC. Chemical sympathectomy blocks androgen biosynthesis during prepuberty. Braz j biol res 1995; 28(10):! 109-12.
13 Moore K, Persaud TVN, Embriologia clínica. 7.ed. Rio de Janeiro: Guanabara Koogan; 2000.
14 Rugh R. The Mouse; its reproduction and development. Minneapolis: Burgess Publishing Co; 1968.
15 Clearmont Y, Harvey SC. Duration of the cycle of the seminifer ous epithelium of normal hypophysectomized and hypophysec
lomized-hormone treated albino rats. Endocrinology 1965;76:80-89.
16 Dallegrave E, Langeloh A, Dalsenter PR, Mantese FG. Toxicidade reprodutiva do herbicida glifosato em ratos. Anais. XVI Latinamerican Congress of Pharmacology, XXXII Brazilian con gress of Pharmacology and experimental Therapeutics, II Iberoamerican Congress of Pharmacology, VII Interamerican Congress of clinicai Pharmacology and therapeutics, Form new
molecules to new methods for health and knowledge in the beginning of new millennium. Águas de Lindóia, São Paulo, Brasil; 2000. Set 13-17. p.375.
17 Mello EB, Mello JRB, Jacoub D, Álvares FT, Carvalho KC, Driemeir D. Efeitos do extrato hidroalcólico (70:30) de Lantana camara Verbenaceare (Linn) sobre o trato reprodutivo de ratos Wistar. Anais. XVI Latinamerican Congress of Pharmacology, XXXII Brazilian congress of Pharmacology and experimental Therapeutics II Iberoamerican Congress of Pharmacology, VII Interamerican Congress of clinicai Pharmacology and therapeu tics. Form new molecules to new methods for health and know edge in the beginning of new millennium. Águas de Lindóia, São Paulo, Brasil; 2000. Set 13-17. p. 376.
18 Almeida, SA, Anselmo-Franci, JA, Rosa e Silva, AAM, Kempinas, WG, Lamano, Carvalho, TL. Prolonged intermittent immobilíza
tion-stress applied throughout sexual development of male rats. In; Rosa e Silva AAM. Br monographs of reproduction & catalog
group 2001, São Paulo: Arte & Ciência; 2001.
19 Mello PRB; Pinto GR, Botelho C. Influência do tabagismo na fer tilidade, gestação e lactação. J pediat 2001; 4(77).
20 Gupta SC, Singh PA, Bajaj AK, Budhraja BK, Tripathi A. Antispermatozal antibodies in leprosy with reference to their morphological patterns. Indian J Lepr 1986; 58 (2):196-201.
21 Sirmuor SK, Verma PK, Singh JN, Okhandiar RP. Semen bio chemistry of leprosy patients Indian j lepr 1997; 69(31:251-4.
22 Hering FLO, Srougi M Urologia: diagnóstico e tratamento. São Paulo: Roca; 1998. cap 34.
23 Faqi AS, Klug A, Merker H), Chahoud I. Ganciclovir induces reproductive hazards in male rats after short-term exposure. Hum
exp toxicol 1997; 16:505-11.
24 Douglas |r JM, Davis LG, Remington ML, Parselsen CA, Perrin EB, Goodman P. Double-blind, placebo-control trial comparing long
term suppressive with short-term oral acyclovir therapy for man agement of recurrent genital herpes. Am j med 1988: 85(Supl 2A);20-5.
25 Lamano Carvalho TL, Lopes RA, Azoubel R, Ferreira AL. Morphometric study of the reversibility of testicle alteration in rats submitted to hypervitaminosis A. Int j vitam nutr 1978; 48(4):316-24.
26 Abd-Allah ARA, Cannam BA, Hamada FMA. The impact of ofloxacina on rat testicular DNA: application of image analysis Pharmac res 2000; 42(2): 145-150.
27 Davis GJ; Mckenzie DVM. Evaluations of ofloxacin. Am i med dez 1989; 29(87) supl 6C:43-46.
28 Yoshida K, Yabe K, Nishida S, Yamamoto N, Ohshima C, Sekiguchi M, Yamada K, Furuhama K. Pharmacokinetic disposition and arthropathic potential of oral ofloxacin in dogs. J vetpharmacol ther 1998; 21:128-132.
29 Crotty KL, May R, Kulvicki A, Kumar D, Neal De jr. The effect of antimicrobial therapy on testicular aspirate flow cytometry. J urol
1995; 153(3 pt1):835-8.
30 Azevedo E, Ribeiro )A, Polonia J, Pontes C. Probable exacerbation of myasthenia gravis by ofloxacin. j neurol 1993; 240:508.
31 Murray CK, Wortmann GW. Trovafloxacín-induced weakness due to a demyelinating polyneuropathy. Souther med j 2000; 93{5):491 -6.
32 Lê Huec JC, Schaeverbeke T, Chauveaus D, Rivel J, Dehais J, Lê Rebeller A. Epidondylitis after treatment with fluoroquinelone
antibiotics. J bone jí sur 1995; 77(2): 293-5.
33 Gasparian JM, Luchi M. Quinolones and tendon ruptures. Souther med j 2000; 93(5): 487-491.
34 Kappel EM, Shakibaei M, Bello A, Stahlmann R. Effects of the Des-F(6)-quinolone garenoxicin (BMS-284756), in comparison to those of ciprofloxacin and ofloxacina, on joint cartilage in immature rats. Antimicrob agents chemother 2002; 46(10);3320-2.
35 0'Donell JA, Gelone SP. Fluorquinolones. Infec dis clin North Am 2000; 14(2):489-51 3, xi.
36 Cohen JS. Peripheral Neuropathy associated with fluorquinolones. Ann pharmacother res reports 2001; 35(12):! 540-7.
37 Stahlmann R. Clinicai toxicological aspects of fluorquinolones. Toxicol Lett 2002; 127(l-3):269-277.
38 Shaefer C et al. Pregnancy outcome after prenatal exposure. Evolution of a case registry of the European Network of teratology
information Services. Eur j obstet gynecol reprod biol 1996; 69(2):83-89.
39 Litt JZ. Fluorquinolones [editorial]. Souther med j 2000; 93(5):325.
40 Drlica K, Schmitz Fj. Therapeutic options in era of decreasing antimicrobial suscptibility. J chemother 2 0 0 2; 14(suppl2):5-12.
levofloxacin in rats and rabbits. Arzneimittelforschung 1992; 43(3A):374-7.
2 Abd-Allah AR, Aíy HA, Moustafa AM, Abdel-Aziz AA, Hamada FM, Adverse testicular effects of some quinolone members in rats.
Pharmacol Res 2000; 41(2);211-219.
3 Fleury RN. Comprometimento visceral na hanseníase. In; Noções de Hansenologia. Bauru: Hospital Lauro de Souza Lima. Centro
de Estudos Dr. Reynaldo Quagliato 1981. p.47-53.
4 Sapota L, Veksel A. Androgenic status in patients with lepromatous leprosy. Br) Urol 1994; 74(2):221-4.
5 Brady TM, Larner J, Minneman KP, Neu HC. Farmacologia humana: da molécula à clínica, 2ed, Rio de )aneiro: Guanabara Koogan; 1 997,
6 Paschoal VDA. Efeitos da ofloxacina no desenvolvimento dos testículos de ratos: estudo cariométricoo. [tese] São José do Rio Preto: Faculdade de Medicina de São José do Rio Preto; 2002.
7 Sala MAM, Matheus M, Valer! VA. A new stereological method for estimation: the thickness of a cellular layet on random sections.Mikroskopile Wien 1981; 38(516):I27-130.
8 Miraglia SM, Hayashí H, Goldfeder EM. Histomorfometria dos testículos de ratos albinos normais em várias idades. Rev bras ciênc morfol 1990; 7(1):8-15.
9 Santos HSL, Azoubel R. Embriologia comparada: texto e atlas, laboticabal: FUNEP; 1996.
10 Sadler TW. Langman/Embriologia médica. 7ed. Rio de Janeiro: Guanabara Koogan; 1997.
11 Zanato VF, Martins MP, Anselmo-Franci )A, Petenusci SO, Carvalho-Lamano TL. Sexual development of male Wistar rats. Braz j med res 1994; 27:1273-1280.
12 Rosa e Silva AAM, Guimarães MA, Lamano-Carvalho TL, Kempinas WC. Chemical sympathectomy blocks androgen biosynthesis during prepuberty. Braz j biol res 1995; 28(10):! 109-12.
13 Moore K, Persaud TVN, Embriologia clínica. 7.ed. Rio de Janeiro: Guanabara Koogan; 2000.
14 Rugh R. The Mouse; its reproduction and development. Minneapolis: Burgess Publishing Co; 1968.
15 Clearmont Y, Harvey SC. Duration of the cycle of the seminifer ous epithelium of normal hypophysectomized and hypophysec
lomized-hormone treated albino rats. Endocrinology 1965;76:80-89.
16 Dallegrave E, Langeloh A, Dalsenter PR, Mantese FG. Toxicidade reprodutiva do herbicida glifosato em ratos. Anais. XVI Latinamerican Congress of Pharmacology, XXXII Brazilian con gress of Pharmacology and experimental Therapeutics, II Iberoamerican Congress of Pharmacology, VII Interamerican Congress of clinicai Pharmacology and therapeutics, Form new
molecules to new methods for health and knowledge in the beginning of new millennium. Águas de Lindóia, São Paulo, Brasil; 2000. Set 13-17. p.375.
17 Mello EB, Mello JRB, Jacoub D, Álvares FT, Carvalho KC, Driemeir D. Efeitos do extrato hidroalcólico (70:30) de Lantana camara Verbenaceare (Linn) sobre o trato reprodutivo de ratos Wistar. Anais. XVI Latinamerican Congress of Pharmacology, XXXII Brazilian congress of Pharmacology and experimental Therapeutics II Iberoamerican Congress of Pharmacology, VII Interamerican Congress of clinicai Pharmacology and therapeu tics. Form new molecules to new methods for health and know edge in the beginning of new millennium. Águas de Lindóia, São Paulo, Brasil; 2000. Set 13-17. p. 376.
18 Almeida, SA, Anselmo-Franci, JA, Rosa e Silva, AAM, Kempinas, WG, Lamano, Carvalho, TL. Prolonged intermittent immobilíza
tion-stress applied throughout sexual development of male rats. In; Rosa e Silva AAM. Br monographs of reproduction & catalog
group 2001, São Paulo: Arte & Ciência; 2001.
19 Mello PRB; Pinto GR, Botelho C. Influência do tabagismo na fer tilidade, gestação e lactação. J pediat 2001; 4(77).
20 Gupta SC, Singh PA, Bajaj AK, Budhraja BK, Tripathi A. Antispermatozal antibodies in leprosy with reference to their morphological patterns. Indian J Lepr 1986; 58 (2):196-201.
21 Sirmuor SK, Verma PK, Singh JN, Okhandiar RP. Semen bio chemistry of leprosy patients Indian j lepr 1997; 69(31:251-4.
22 Hering FLO, Srougi M Urologia: diagnóstico e tratamento. São Paulo: Roca; 1998. cap 34.
23 Faqi AS, Klug A, Merker H), Chahoud I. Ganciclovir induces reproductive hazards in male rats after short-term exposure. Hum
exp toxicol 1997; 16:505-11.
24 Douglas |r JM, Davis LG, Remington ML, Parselsen CA, Perrin EB, Goodman P. Double-blind, placebo-control trial comparing long
term suppressive with short-term oral acyclovir therapy for man agement of recurrent genital herpes. Am j med 1988: 85(Supl 2A);20-5.
25 Lamano Carvalho TL, Lopes RA, Azoubel R, Ferreira AL. Morphometric study of the reversibility of testicle alteration in rats submitted to hypervitaminosis A. Int j vitam nutr 1978; 48(4):316-24.
26 Abd-Allah ARA, Cannam BA, Hamada FMA. The impact of ofloxacina on rat testicular DNA: application of image analysis Pharmac res 2000; 42(2): 145-150.
27 Davis GJ; Mckenzie DVM. Evaluations of ofloxacin. Am i med dez 1989; 29(87) supl 6C:43-46.
28 Yoshida K, Yabe K, Nishida S, Yamamoto N, Ohshima C, Sekiguchi M, Yamada K, Furuhama K. Pharmacokinetic disposition and arthropathic potential of oral ofloxacin in dogs. J vetpharmacol ther 1998; 21:128-132.
29 Crotty KL, May R, Kulvicki A, Kumar D, Neal De jr. The effect of antimicrobial therapy on testicular aspirate flow cytometry. J urol
1995; 153(3 pt1):835-8.
30 Azevedo E, Ribeiro )A, Polonia J, Pontes C. Probable exacerbation of myasthenia gravis by ofloxacin. j neurol 1993; 240:508.
31 Murray CK, Wortmann GW. Trovafloxacín-induced weakness due to a demyelinating polyneuropathy. Souther med j 2000; 93{5):491 -6.
32 Lê Huec JC, Schaeverbeke T, Chauveaus D, Rivel J, Dehais J, Lê Rebeller A. Epidondylitis after treatment with fluoroquinelone
antibiotics. J bone jí sur 1995; 77(2): 293-5.
33 Gasparian JM, Luchi M. Quinolones and tendon ruptures. Souther med j 2000; 93(5): 487-491.
34 Kappel EM, Shakibaei M, Bello A, Stahlmann R. Effects of the Des-F(6)-quinolone garenoxicin (BMS-284756), in comparison to those of ciprofloxacin and ofloxacina, on joint cartilage in immature rats. Antimicrob agents chemother 2002; 46(10);3320-2.
35 0'Donell JA, Gelone SP. Fluorquinolones. Infec dis clin North Am 2000; 14(2):489-51 3, xi.
36 Cohen JS. Peripheral Neuropathy associated with fluorquinolones. Ann pharmacother res reports 2001; 35(12):! 540-7.
37 Stahlmann R. Clinicai toxicological aspects of fluorquinolones. Toxicol Lett 2002; 127(l-3):269-277.
38 Shaefer C et al. Pregnancy outcome after prenatal exposure. Evolution of a case registry of the European Network of teratology
information Services. Eur j obstet gynecol reprod biol 1996; 69(2):83-89.
39 Litt JZ. Fluorquinolones [editorial]. Souther med j 2000; 93(5):325.
40 Drlica K, Schmitz Fj. Therapeutic options in era of decreasing antimicrobial suscptibility. J chemother 2 0 0 2; 14(suppl2):5-12.
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