Keywords
bacteremia
Pseudomonas aeruginosa
serotyping
pyocintyping
ribotyping
How to Cite
Abstract
Between September 11 and 20, 1996, an outbreak of bacteremia occurred in patients undergoing hemodialysis at one dialysis center in Campinas, São Paulo, Brazil. Water and dialysate samples as well as blood samples from patients with bacteremia were collected for bacteriological analysis. All Pseudomonas
aeruginosa strains were serotyped and pyocin-typed. P. aeruginosa strains belonging to the same serotype (O15) and pyocin type (P10) were submitted to antimicrobial susceptibility testing and to ribotyping using two restriction enzymes, EcoRI and BamHI. The high concentrations of bacteria detected in all hemodialyzers
and their heavy rate of contamination (86.7%) by Pseudomonas aeruginosa or Burkholderia cepacia showed that this dialysis center was operating in inadequate conditions. One strain of P. aeruginosa isolated from hemodialyzer and the strains recovered from blood cultures of patients showed similar phenotypical and genetic traits which suggest a common source of infection in this oubreak. We report the potential risk for patients undergoing hemodialysis to acquire infections due to the inadequate practice of reusing disposable dialyzers during the reprocessing procedures.
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