Keywords
monoclonal antibodies
oysters
How to Cite
Abstract
V. cholerae serovar O1 is the etiologic agent responsible for pandemic cholera, and it is considered the most important human pathogenic Vibrio. The symptoms presented by patients affected by this bacterium vary from mild diarrhea to severe disease, and may even lead to death. Among the diverse types of seafood, the oysters pose as an important means for cholera transmission. Traditional methods used to detect V. cholerae are laborious and time-consuming, and there is an urgent need to establish a rapid, sensitive, specific, simple, and low-cost testing . The objective of this trial was, therefore, to assess the performance of a slide agglutination test using latex particles sensitized with specific monoclonal antibodies (MAb) for detecting V. cholerae O1 in experimentally-contaminated oysters. The slide agglutination test based on sensitized latex detected 1.2X102 CFU of bacteria (dilution 1:32). Oyster samples used in the present study, for being experimentally contaminated, were originally free of V. cholerae, but other bacteria were found, such as: Proteus mirabilis, Pseudomonas spp, and other vibrios. The present study demonstrated that the period of time needed to verify the food contamination with V. cholerae is 18 hours, taking in consideration that traditional methods require an average of 7-day period for getting the final results. The produced MAb presented 100% of specificity to V. cholerae 01.References
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