Keywords
aflatoxin M1
determination
thin layer chromatography
immunoaffinity column
How to Cite
Abstract
An immunoaffinity column for cleanup and a thin layer chromatographic method for determining low aflatoxin M1 concentration in milk were performed in our laboratory. The parameters, such as recovery,
repeatibility, detection and quantification limit were evaluated to carry out this method. Based on spiked
samples, the values of recoveries ranged from 85.83 to 73.86 % at levels of 0.01-0.5 μg/L, respectively, and
the relative standard deviation for repeatibility (RSDr) ranged from 7.73 to 2.08%. The quantification limit
established in this method was 0,02 μg/L. The results of analysed samples by this method demonstrated
satisfatory correlation when compared with HPLC. In addition, the AOAC method (1975) was also performed,
resulting in detection and quantification limits of 0.2 and 0.3 μg/L, respectively, indicating low sensibility
of this method. Immunoaffinity column cleanup gave excellent results for recovery, sensibility and sample
throughput.
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